Name: the mouse forced swim test
Uploaded: 2012-01-29T14:00:00
Description: Watch this Scientific Journal Video about The Mouse Forced Swim Test at JoVE.com

Bu çalışmada, hibe NIHM, R01 MH091816 ve R21 MH084043 TDG için tarafından desteklenen olmuştur.

1. Gereç ve Yöntem 1.1. Su tankları Bu malzeme, cam daha iyi tankları ve kazaların sık sık hareket dayanabilmesi olarak silindirik tanklar (30 cm yükseklik x 20 cm çapında), şeffaf pleksiglas inşa bizim laboratuvarda fare zorla yüzmeye testi (FST) için gerekli. Su seviyesi 15 cm alttan ve su hacmini fareler tutarlı olmasını sağlamak için tank üzerinde işaretlenmiş olmalıdır. Tank sayısı ideal ilk seti kullanımda iken, ikinci bir su deposu se...

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FST (bazen Porsolt yüzmeye testi denilen), sıçanlar için ilk geliştirilen ve fareler için Porsolt ve meslektaşları 12,13 tarafından güncellenmiştir . Laboratuvarımızda başarılı yukarıda açıklanan protokole ek olarak, büyük ölçüde ince test değişiklikler bir dizi (tam bir incelemesi 1 için bkz. Hascoët ve BouriN) yayınlanmıştır . Anti-depresan ilaçlar ve etkileri temel ve preklinik araştırma 3,14-16 çeşitli davranışsal ve nörobiyolojik manipülasyon...

<ul><li> Su tankları </li><li> Termometre </li><li> Timer </li><li> Video Kamera </li><li> White Noise Jeneratör (isteğe bağlı) </li><li> Kağıt Kurutma </li></ul>

the mouse forced swim test

Zorla yüzmeye testi, antidepresan ilaçlar, antidepresan etkinliği, yeni bileşiklerin ve devletler gibi depresif kılan veya önlemeyi amaçlayan deneysel manipülasyonlar değerlendirilmesi için kullanılan bir kemirgen davranış testi. Fare kaçınılmaz şeffaf bir tank içinde su ile doldurulur ve bir kaçış ilgili hareketlilik davranışı ölçülür yerleştirilir. Zorla yüzmeye testi güvenilir bir biçimde yürütmek için basit ve minimal özel ekipman gerektirir. Zorunlu yüzme testinin başarılı bir şekilde uygulanması, haksız stres farelere belirli usul detayları ve minimizasyonu bağlılık gerektirir. Protokol tanımı ve eşlik eden video, biz bunları önlemek için, sonuçların yorumlanması ve zararlı olabilecek potansiyel tuzaklar vurgu yaparak bu testin fare sürüm yapmak için nasıl. Ayrıca, test davranışlar nasıl değerlendirilir.

Watch this Scientific Journal Video about The Mouse Forced Swim Test at JoVE.com

The Mouse Forced Swim Test

Zorla yüzmeye testi kemirgenlerde potansiyel antidepresan etkinliğini değerlendirmek için deneysel bir yaklaşım olarak onaylanmıştır. Deneysel hayvan, su ve kaçış ilgili hareketlilik davranış bir tank yerleştirilir rakamlarla gösterir. Bu testin fare sürümü için ortak prosedürler açıklanmıştır.

Fare Zorla Swim Testi

The forced swim test is a rodent behavioral test used for evaluation of antidepressant drugs, antidepressant efficacy of new compounds, and experimental ...

Research

JoVE Journal

Neuroscience

One Dimensional Turing-Like Handshake Test for Motor Intelligence

Motor İstihbarat Bir Boyutlu Turing Gibi El Sıkışma Testi

In the Turing test, a computer model is deemed to "think intelligently" if it can generate answers that are not distinguishable from those of a human.  ...

Multiple-mouse Neuroanatomical Magnetic Resonance Imaging

Çoklu-fare nöroanatomik Manyetik Rezonans Görüntüleme

The field of mouse phenotyping with magnetic resonance imaging (MRI) is rapidly growing, motivated by the need for improved tools for characterizing and ...

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Nöritik plaklar Alzheimer Hastalığı Fare Modeli Algılama

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T-maze Forced Alternation and Left-right Discrimination Tasks for Assessing Working and Reference Memory in Mice

T-labirent Zorla Değişim ve Çalışma ve Fareler Referans Bellek değerlendirilmesi için sağ-sol Ayrımcılık Görevler

Forced alternation and left-right discrimination tasks using the T-maze have been widely used to assess working and reference memory, respectively, in ...

Tissue Preparation and Immunostaining of Mouse Sensory Nerve Fibers Innervating Skin and Limb Bones

Deri ve Kol ve bacak kemikleri innerve Fare Duyusal Sinir Lif doku hazırlanması ve immün

Detection and primary processing of physical, chemical and thermal sensory stimuli by peripheral sensory nerve fibers is key to sensory perception in ...

The Tail Suspension Test

Kuyruk Süspansiyon Testi

The tail-suspension test is a mouse behavioral test useful in the screening of potential antidepressant drugs, and assessing of other manipulations that ...

Stereotaxic Infusion of Oligomeric Amyloid-beta into the Mouse Hippocampus

Fare Hippocampus içine Oligomerik Amiloid-beta Stereotaksik İnfüzyon

Alzheimer&#8217;s disease is a neurodegenerative disease affecting the aging population. A key neuropathological feature of the disease is the ...

The Fibular Nerve Injury Method: A Reliable Assay to Identify and Test Factors That Repair Neuromuscular Junctions

Fibula Sinir Yaralanması Yöntem: Güvenilir Testi tanımak ve Test O Tamir Nöromüsküler Junctions Faktörleri

The neuromuscular junction (NMJ) undergoes deleterious structural and functional changes as a result of aging, injury and disease. Thus, it is imperative ...

Automated Analysis of C. elegans Swim Behavior Using CeleST Software

Automated Analysis of C. elegans Swim Behavior Using CeleST Software

ve Otomatik Analizi<em&gt; C. elegans</emCelest Yazılım Kullanma&gt; Deniz Davranışı

Dissecting the neuronal and neuromuscular circuits that regulate behavior remains a major challenge in biology. The nematode Caenorhabditis elegans has ...

Diffuse Reflectance Spectroscopy: Getting the Capillary Refill Test Under One's Thumb

Diffüz yansıma spektroskopisi: bir parmağının altında kılcal damar yeniden doldurmak Test alma

The capillary refill test was introduced in 1947 to help estimate circulatory status in critically ill patients. Guidelines commonly state that refill ...