Name: assessing hepatic metabolic changes during progressive colonization of germ-free mouse by 1h nmr spectroscopy
Uploaded: 2011-12-15T13:00:00
Description: Watch this Scientific Journal Video about Assessing Hepatic Metabolic Changes During Progressive Colonization of Germ-free Mouse by 1H NMR Spectroscopy at JoVE.com

Все спектров ЯМР используется в качестве иллюстративных примеров взяты из ранее опубликованных исследований 7, которая выполнена при финансовой поддержке компании Nestle.

1. Колонизация неинфицированных животных и отбора образцов <ol><li> Удалить неинфицированных животных из изоляторов и их размещение в обычной комнате хозяйства в клетках, оборудованных фильтром перед обычных животных, которые будут служить в качестве контроля (рис. 1). </li><li> Смешайте поло...

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	<li>Cani, P. D., Delzenne, N. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Gut+microflora+as+a+target+for+energy+and+metabolic.">Gut microflora as a target for energy and metabolic.</a> Curr. Opin. Clin. Nutr. Metab. Care. 10, 729-734 (2007).</li><li>Ley, R. E., Turnbaugh, P. J., Klein, S., Gordon, J. I. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Microbial+ecology:+human+gut+microbes+associated+with+obesity.">Microbial ecology: human gut microbes associated with obesity.</a> Nature. 444, 1022-1023 (2006).</li><li>Raoult, D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Obesity+pandemics+and+the+modification+of+digestive+bacterial+flora.">Obesity pandemics and the modification of digestive bacterial flora.</a> Eur. J. Clin. Microbiol. Infect. Dis. 27, 631-634 (2008).</li><li>Turnbaugh, P. J., Backhed, F., Fulton, L., Gordon, J. I. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Diet-induced+obesity+is+linked+to+marked+but+reversible+alterations+in+the+mouse+distal+gut+microbiome.">Diet-induced obesity is linked to marked but reversible alterations in the mouse distal gut microbiome.</a> Cell. Host. Microbe. 3, 213-223 (2008).</li><li>Balcombe, J. P., Barnard, N. D., Sandusky, C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Laboratory+routines+cause+animal+stress.">Laboratory routines cause animal stress.</a> Contemp. Top. Lab. Anim. Sci. 43, 42-51 (2004).</li><li>Muyzer, G., Smalla, K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Application+of+denaturing+gradient+gel+electrophoresis+(DGGE)+and+temperature+gradient+gel+electrophoresis+(TGGE)+in+microbial+ecology.">Application of denaturing gradient gel electrophoresis (DGGE) and temperature gradient gel electrophoresis (TGGE) in microbial ecology.</a> Antonie van Leeuwenhoek. 73, 127-141 (1998).</li><li>Claus, S. P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Colonization-induced+host-gut+microbial+metabolic+interaction.">Colonization-induced host-gut microbial metabolic interaction.</a> MBio. 2, (2011).</li><li>Waters, N. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=High-resolution+magic+angle+spinning+1H+NMR+spectroscopy+of+intact+liver+and+kidney:+optimization+of+sample+preparation+procedures+and+biochemical+stability+of+tissue+during+spectral+acquisition.">High-resolution magic angle spinning 1H NMR spectroscopy of intact liver and kidney: optimization of sample preparation procedures and biochemical stability of tissue during spectral acquisition.</a> Anal. Biochem. 282, 16-23 (2000).</li><li>Bollard, M. E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=High-resolution+1H+and+1H-13C+magic+angle+spinning+NMR+spectroscopy+of+rat+liver.">High-resolution 1H and 1H-13C magic angle spinning NMR spectroscopy of rat liver.</a> Magnetic resonance in medicine. 44, 201-207 (2000).</li><li>Lindon, J. C., Holmes, E., Nicholson, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Pattern+recognition+methods+and+applications+in+biomedical+magnetic+resonance.">Pattern recognition methods and applications in biomedical magnetic resonance.</a> Progress in Nuclear Magnetic Resonance Spectroscopy. 39, 1-40 (2001).</li><li>Tate, A. R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Distinction+between+normal+and+renal+cell+carcinoma+kidney+cortical+biopsy+samples+using+pattern+recognition+of+(1)H+magic+angle+spinning+(MAS)+NMR+spectra.">Distinction between normal and renal cell carcinoma kidney cortical biopsy samples using pattern recognition of (1)H magic angle spinning (MAS) NMR spectra.</a> NMR. Biomed. 13, 64-71 (2000).</li><li>Wang, Y. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Topographical+variation+in+metabolic+signatures+of+human+gastrointestinal+biopsies+revealed+by+high-resolution+magic-angle+spinning+1H+NMR+spectroscopy.">Topographical variation in metabolic signatures of human gastrointestinal biopsies revealed by high-resolution magic-angle spinning 1H NMR spectroscopy.</a> Journal of Proteome Research. 6, 3944-3951 (2007).</li><li>Meiboom, S., Gill, D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Modified+spin-echo+method+for+measuring+nuclear+relaxation+times.">Modified spin-echo method for measuring nuclear relaxation times.</a> The review of scientific instruments. 29, 688-691 (1958).</li><li>Nicholson, J. K., Holmes, E., Wilson, I. D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Gut+microorganisms,+mammalian+metabolism+and+personalized+health+care.">Gut microorganisms, mammalian metabolism and personalized health care.</a> Nat. Rev. Microbiol. 3, 431-438 (2005).</li><li>Martin, F. P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Effects+of+probiotic+Lactobacillus+paracasei+treatment+on+the+host+gut+tissue+metabolic+profiles+probed+via+magic-angle-spinning+NMR+spectroscopy.">Effects of probiotic Lactobacillus paracasei treatment on the host gut tissue metabolic profiles probed via magic-angle-spinning NMR spectroscopy.</a> Journal of Proteome Research. 6, 1471-1481 (2007).</li><li>Swann, J. R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Variation+in+Antibiotic-Induced+Microbial+Recolonization+Impacts+on+the+Host+Metabolic+Phenotypes+of+Rats.">Variation in Antibiotic-Induced Microbial Recolonization Impacts on the Host Metabolic Phenotypes of Rats.</a> J. Proteome. Res. , (2011).</li><li>Jacobs, D. M., Gaudier, E., van Duynhoven, J., Vaughan, E. E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Non-digestible+food+ingredients,+colonic+microbiota+and+the+impact+on+gut+health+and+immunity:+a+role+for+metabolomics.">Non-digestible food ingredients, colonic microbiota and the impact on gut health and immunity: a role for metabolomics.</a> Curr. Drug. Metab. 10, 41-54 (2009).</li><li>Beckonert, O. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=High-resolution+magic-angle-spinning+NMR+spectroscopy+for+metabolic+profiling+of+intact+tissues.">High-resolution magic-angle-spinning NMR spectroscopy for metabolic profiling of intact tissues.</a> Nat. Protoc. 5, 1019-1032 (2010).</li><li>Hooper, L. V., Sansonetti, P., Zychlinsky, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=.">.</a> Methods in microbiology. 31, 559-589 (2002).</li><li>Rahija, R. J., Fox, J. G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Ch.+7.">Ch. 7.</a> The mouse in biomedical research. , 217-234 (2007).</li><li>Goodwin, B. L., Ruthven, C. R., Sandler, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Gut+flora+and+the+origin+of+some+urinary+aromatic+phenolic+compounds.">Gut flora and the origin of some urinary aromatic phenolic compounds.</a> Biochemical Pharmacology. 47, 2294-2297 (1994).</li><li>Koopman, J. P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term='Normalization'+of+germfree+mice+after+direct+and+indirect+contact+with+mice+having+a+'normal'+intestinal+microflora.">'Normalization' of germfree mice after direct and indirect contact with mice having a 'normal' intestinal microflora.</a> Lab Anim. 20, 286-290 (1986).</li><li>Nishikata, N., Shikata, N., Kimura, Y., Noguchi, Y. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Dietary+lipid-dependent+regulation+of+de+novo+lipogenesis+and+lipid+partitioning+by+ketogenic+essential+amino+acids+in+mice.">Dietary lipid-dependent regulation of de novo lipogenesis and lipid partitioning by ketogenic essential amino acids in mice.</a> Nutrition and Diabetes. 1, 1-12 (2011).</li><li>Spagou, K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+GC-MS+metabolic+profiling+study+of+plasma+samples+from+mice+on+low-+and+high-fat+diets.">A GC-MS metabolic profiling study of plasma samples from mice on low- and high-fat diets.</a> J. Chromatogr. B. Analyt. Technol. Biomed. Life. Sci. 879, 1467-1475 (2011).</li><li>Sanchez-Patan, F., Monagas, M., Moreno-Arribas, M. V., Bartolome, B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Determination+of+microbial+phenolic+acids+in+human+faeces+by+UPLC-ESI-TQ+MS.">Determination of microbial phenolic acids in human faeces by UPLC-ESI-TQ MS.</a> J. Agric. Food. Chem. 59, 2241-2247 (2011).</li><li>Roux, A., Lison, D., Junot, C., Heilier, J. F. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Applications+of+liquid+chromatography+coupled+to+mass+spectrometry-based+metabolomics+in+clinical+chemistry+and+toxicology:+A+review.">Applications of liquid chromatography coupled to mass spectrometry-based metabolomics in clinical chemistry and toxicology: A review.</a> Clin. Biochem. 44, 119-135 (2011).</li><li>Ryan, D., Robards, K., Prenzler, P. D., Kendall, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Recent+and+potential+developments+in+the+analysis+of+urine:+a+review.">Recent and potential developments in the analysis of urine: a review.</a> Anal. Chim. Acta. 684, 8-20 (2011).</li><li>Nagayama, K., Wuthrich, K., Bachmann, P., Ernst, R. R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Two-dimensional+J-resolved+1H+n.m.r.+spectroscopy+for+studies+of+biological+macromolecules.">Two-dimensional J-resolved 1H n.m.r. spectroscopy for studies of biological macromolecules.</a> Biochem. Biophys. Res. Commun. 78, 99-105 (1977).</li><li>Aue, W. P., Bartholdi, E., Ernst, R. R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Two-dimensional+spectroscopy.+Application+to+nuclear+magnetic+resonance.">Two-dimensional spectroscopy. Application to nuclear magnetic resonance.</a> J. Chem. Phys. 64, 2229-2246 (1975).</li><li>Bodenhausen, G., Ruben, D. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Natural+abundance+15N+NMR+by+enhanced+heteronuclear+spectroscopy.">Natural abundance 15N NMR by enhanced heteronuclear spectroscopy.</a> Chemical. Physics. Letters. 69, 185-189 (1980).</li><li>Fan, T. W. -. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Metabolite+profiling+by+one-+and+two-dimensional+NMR+analysis+of+complex+mixtures.">Metabolite profiling by one- and two-dimensional NMR analysis of complex mixtures.</a> Progress in nuclear magnetic resonance spectroscopy. 28, 161-219 (1996).</li><li>Fan, T., Lane, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Structure-based+profiling+of+metabolites+and+isotopomers+by+NMR.">Structure-based profiling of metabolites and isotopomers by NMR.</a> Progress in Nuclear Magnetic Resonance Spectroscopy. 52, 48-48 (2008).</li><li>Fonville, J. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+evolution+of+partial+least+squares+models+and+related+chemometric+approaches+in+metabonomics+and+metabolic+phenotyping.">The evolution of partial least squares models and related chemometric approaches in metabonomics and metabolic phenotyping.</a> Journal of Chemometrics. 24, 636-649 (2010).</li><li>Merrifield, C. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+metabolic+system-wide+characterisation+of+the+pig:+a+model+for+human+physiology.">A metabolic system-wide characterisation of the pig: a model for human physiology.</a> Mol. Biosyst. , (2011).</li><li>Tugnoli, V. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Molecular+characterization+of+human+gastric+mucosa+by+HR-MAS+magnetic+resonance+spectroscopy.">Molecular characterization of human gastric mucosa by HR-MAS magnetic resonance spectroscopy.</a> International Journal of Molecular Medicine. 14, 1065-1071 (2004).</li><li>Sitter, B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Comparison+of+HR+MAS+MR+spectroscopic+profiles+of+breast+cancer+tissue+with+clinical+parameters.">Comparison of HR MAS MR spectroscopic profiles of breast cancer tissue with clinical parameters.</a> NMR Biomed. 19, 30-40 (2006).</li><li>Beckonert, O. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Metabolic+profiling,+metabolomic+and+metabonomic+procedures+for+NMR+spectroscopy+of+urine,+plasma,+serum+and+tissue+extracts.">Metabolic profiling, metabolomic and metabonomic procedures for NMR spectroscopy of urine, plasma, serum and tissue extracts.</a> Nat. Protoc. 2, 2692-2703 (2007).</li></ol>

В этом протоколе, мы описали прогрессивные процедуры колонизации в открытую среду для дальнейшего изучения воздействия микрофлоры кишечника на печеночный метаболизм оценивается 1 H HR MAS ЯМР профилирования интактных биопсии. Различные методы колонизации были описаны в литератур...

Таблица специфических реагентов и оборудования: <table border="1"><tbody><tr><th> Название реагента </th><th> Компания </th><th> Номер по каталогу </th><th> Комментарии </th></tr><tr><td> 2,5 мм микропробирок </td><td> Новая эра </td><td> NE-H5/2.5-V-Br </td><td></td></tr><tr><td> 1,7 мм капиллярная трубка </td><td> Sigma-Aldrich </td><td> NORS175001 </td><td></td></tr><tr><td> Капиллярная адаптер </td><td> Новая эра </td><td> NE-325-5/1.7 </td><td></td></tr><tr><td> Добыча стержень </td><td> Новая эра </td><td> NE-341-5 </td><td></td></tr><tr><td> HR-MAS ротора BL4 с 50 мкл сферической Spacer тефлоновым комплект </td><td> Bruker </td><td> HZ07213 </td><td></td></tr><tr><td> Набор для 50 мкл вставками </td><td> Bruker </td><td> B2950 </td><td></td></tr><tr><td> Advance III 600МГц ЯМР </td><td> Bruker </td><td></td><td></td></tr><tr><td> 1 H HR MAS ЯМР твердого штыря </td><td> Bruker </td><td></td><td></td></tr><tr><td> Оксид дейтерия 99,9% </td><td> Sigma-Aldrich </td><td> 530 867-1L </td><td></td></tr><tr><td> 3 - (триметилсилил) пропионовой кислотно-D 4 (TSP) </td><td> Sigma-Aldrich </td><td> 269913 </td><td></td></tr></tbody></table>

assessing hepatic metabolic changes during progressive colonization of germ-free mouse by 1h nmr spectroscopy

It is well known that gut bacteria contribute significantly to the host homeostasis, providing a range of benefits such as immune protection and vitamin synthesis. They also supply the host with a considerable amount of nutrients, making this ecosystem an essential metabolic organ. In the context of increasing evidence of the link between the gut flora and the metabolic syndrome, understanding the metabolic interaction between the host and its gut microbiota is becoming an important challenge of modern biology.1-4
 Colonization (also referred to as normalization process) designates the establishment of micro-organisms in a former germ-free animal. While it is a natural process occurring at birth, it is also used in adult germ-free animals to control the gut floral ecosystem and further determine its impact on the host metabolism. A common procedure to control the colonization process is to use the gavage method with a single or a mixture of micro-organisms. This method results in a very quick colonization and presents the disadvantage of being extremely stressful5. It is therefore useful to minimize the stress and to obtain a slower colonization process to observe gradually the impact of bacterial establishment on the host metabolism.
 In this manuscript, we describe a procedure to assess the modification of hepatic metabolism during a gradual colonization process using a non-destructive metabolic profiling technique. We propose to monitor gut microbial colonization by assessing the gut microbial metabolic activity reflected by the urinary excretion of microbial co-metabolites by 1H NMR-based metabolic profiling. This allows an appreciation of the stability of gut microbial activity beyond the stable establishment of the gut microbial ecosystem usually assessed by monitoring fecal bacteria by DGGE (denaturing gradient gel electrophoresis).6 The colonization takes place in a conventional open environment and is initiated by a dirty litter soiled by conventional animals, which will serve as controls. Rodents being coprophagous animals, this ensures a homogenous colonization as previously described.7
 Hepatic metabolic profiling is measured directly from an intact liver biopsy using 1H High Resolution Magic Angle Spinning NMR spectroscopy. This semi-quantitative technique offers a quick way to assess, without damaging the cell structure, the major metabolites such as triglycerides, glucose and glycogen in order to further estimate the complex interaction between the colonization process and the hepatic metabolism7-10. This method can also be applied to any tissue biopsy11,12.

Watch this Scientific Journal Video about Assessing Hepatic Metabolic Changes During Progressive Colonization of Germ-free Mouse by 1H NMR Spectroscopy at JoVE.com

Assessing Hepatic Metabolic Changes During Progressive Colonization of Germ-free Mouse by 1H NMR Spectroscopy

Прогрессивная процедура колонизации описывается для дальнейшей оценки его влияния на метаболизм печеночных хоста. Колонизация контролируется, не invasively путем оценки экскреции микробных метаболитов совместного с помощью ЯМР основе метаболических профилей в то время как печеночный метаболизм оценивается Высокое разрешение магического угла Спиннинг (HR MAS) ЯМР профилирования интактных биопсии.

Оценка изменения Печеночная Метаболический Во Прогрессивная Колонизация зародыш без мыши по 1 Н-ЯМР-спектроскопии

It is well known that gut bacteria contribute significantly to the host homeostasis, providing a range of benefits such as immune protection and vitamin ...

Research

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Immunology and Infection

Assessing Hepatic Metabolic Changes During Progressive Colonization of Germ-free Mouse by 1H NMR Spectroscopy

Quantification of Fungal Colonization, Sporogenesis, and Production of Mycotoxins Using Kernel Bioassays

Количественная грибковой колонизации, спорогенеза, и производство микотоксинов Использование ядра Биопробы

The rotting of grains by seed-infecting fungi poses one of the greatest economic challenges to cereal production worldwide, not to mention serious risks ...

Colonization of Euprymna scolopes Squid by Vibrio fischeri

Colonization of Euprymna scolopes Squid by Vibrio fischeri

Колонизация Euprymna scolopes Squid по Vibrio fischeri</em

Specific bacteria are found in association with animal tissue1-5. Such host-bacterial associations (symbioses) can be detrimental (pathogenic), have no ...

Detection of MicroRNAs in Microglia by Real-time PCR in Normal CNS and During Neuroinflammation

Обнаружение микроРНК в Микроглия по ПЦР в реальном времени в нормальном ЦНС и во время Neuroinflammation

Microglia are cells of the myeloid lineage that reside in the central nervous system (CNS)1. These cells play an important role in pathologies of many ...

Characterization of Inflammatory Responses During Intranasal Colonization with Streptococcus pneumoniae

Characterization of Inflammatory Responses During Intranasal Colonization with Streptococcus pneumoniae

Характеристика воспалительных реакций во время интраназальной колонизации стрептококковой пневмонией

Nasopharyngeal colonization by Streptococcus pneumoniae is a prerequisite to invasion to the lungs or bloodstream1. This organism is capable of colonizing ...

Assessing Anti-fungal Activity of Isolated Alveolar Macrophages by Confocal Microscopy

Оценка противогрибковое действие изолированных альвеолярных макрофагов с помощью конфокальной микроскопии

The lung is an interface where host cells are routinely exposed to microbes and microbial products. Alveolar macrophages are the first-line phagocytic ...

Assessing Bacterial Invasion of Cardiac Cells in Culture and Heart Colonization in Infected Mice Using Listeria monocytogenes

Assessing Bacterial Invasion of Cardiac Cells in Culture and Heart Colonization in Infected Mice Using Listeria monocytogenes

Оценка бактериального вторжения сердечных клеток в культуре и колонизации сердца у инфицированных мышей с использованием листерий моноцитогенов

Listeria monocytogenes is a Gram-positive facultative intracellular pathogen that is capable of causing serious invasive infections in immunocompromised ...

Selective Harvesting of Marginating-hepatic Leukocytes

Селективный Заготовка Marginating-печеночной Лейкоциты

Marginating-hepatic (MH) leukocytes (leukocytes adhering to the sinusoids of the liver), were shown to exhibit unique composition and characteristics ...

A Murine Model of Group B Streptococcus Vaginal Colonization

A Murine Model of Group B Streptococcus Vaginal Colonization

Мышиной модели группы B<em&gt; Streptococcus</em&gt; Вагинальный Колонизация

Streptococcus agalactiae (group B Streptococcus, GBS), is a Gram-positive, asymptomatic colonizer of the human gastrointestinal tract and vaginal tract of ...

Molecular Diffusion in Plasma Membranes of Primary Lymphocytes Measured by Fluorescence Correlation Spectroscopy

Молекулярная диффузия в плазматических мембранах первичных лимфоцитах Измеренные с помощью флуоресцентной корреляционной спектроскопии

Fluorescence correlation spectroscopy (FCS) is a powerful technique for studying the diffusion of molecules within biological membranes with high spatial ...

Detection of Enterohemorrhagic Escherichia Coli Colonization in Murine Host by Non-invasive In Vivo Bioluminescence System

Detection of Enterohemorrhagic Escherichia Coli Colonization in Murine Host by Non-invasive In Vivo Bioluminescence System

Обнаружение энтерогеморрагические Escherichia Coli колонизации в мышиных принимающей системой неинвазивные в Vivo биолюминесценции

Enterohemorrhagic E. coli (EHEC) O157:H7, which is a foodborne pathogen that causesdiarrhea, hemorrhagic colitis (HS), and hemolytic uremic syndrome ...