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DOI :
10.3791/3643-v
•
10:02 min
February 25th, 2012
Chapters
0:05
Title
2:20
Cell Lysis
3:30
Binding to Rabbit IgG-agarose
4:53
TEV Protease Cleavage
1:30
Transfection
7:50
Biotin Elution of the Streptavidin Binging Peptide and Bait Protein
5:48
Binding to Immobilized Streptavidin Beads
串联亲和纯化的蛋白结合合作伙伴的标识是稳健的做法。由于概念证明,这种方法的应用以及特色的翻译起始因子eIF4E共沉淀的宿主细胞因子参与翻译起始。这种方法是很容易适应任何细胞或病毒蛋白。
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