Name: immunohistological labeling of microtubules in sensory neuron dendrites, tracheae, and muscles in the drosophila larva body wall
Uploaded: 2011-11-10T13:00:00
Description: Watch this Scientific Journal Video about Immunohistological Labeling of Microtubules in Sensory Neuron Dendrites, Tracheae, and Muscles in the Drosophila Larva Body Wall at JoVE.com

我々は、資金調達のために理研に感謝。 P10 - Gal4のはアランヴィンセント（大学ポールサバティエ、トゥールーズ、フランス）の一種の贈り物だった。

1。試薬の調製 開始前の注意：解剖と免疫組織化学的染色は、磁気チャンバー内で実施され、幼虫は、特殊形状の昆虫ピンを使用して釘付けされる。磁気室の建設、及びこれらのピンの準備手順の詳細については、関連する参照14,15に記載されています。簡単に言うと、1x1cmの正方形の穴が磁気シートと小さなチャンバーを作るために、シートの背面に...

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理解するために複雑な細胞の形状が達成される方法、それは正確にアッセイの微小管の組織にできることが重要です。ここでは、樹状突起分枝のニューロン感覚樹状突起のアッセイの微小管の組織に強固な免疫標識法を説明します。感覚ニューロンを染色に加えて、この方法では気管、筋肉や他の体壁組織の強固な免疫組織染色を実現しています。 我々は、DAニューロン...

<table border="1"><tbody><tr><th>試薬の名前</th><th>会社</th><th>カタログ 数は</th><th>コメント （省略可能） </th></tr><tr><td>鉗子</td><td>デュモン</td><td> 11251〜20 </td><td></td></tr><tr><td> Microscissors </td><td> FST </td><td> 15000〜08 </td><td></td></tr><tr><td>マウス抗α-チューブリン（クローン：DM1A） </td><td>シグマ</td><td> T9026 </td><td>希釈1000分の1 </td></tr><tr><td>マウス抗Futsch（クローン：22C10）、 上清</td><td>発達 研究 ハイブリドーマバンク</td><td> 22C10 </td><td>希釈1000分の1 </td></tr><tr><td>ラット抗CD8（クローン：5H10） </td><td> Caltag </td><td> MCD0800 </td><td>希釈1000分の1 </td></tr><tr><td>のAlexa Fluor ® 488抗マウスIgG </td><td>インビトロジェン</td><td> - 11001 </td><td>希釈500分の1 </td></tr><tr><td> Cy3標識抗ラットIgG </td><td>ジャクソンイムノ</td><td> 712-166-150 </td><td>希釈200分の1 </td></tr></tbody></table>

immunohistological labeling of microtubules in sensory neuron dendrites, tracheae, and muscles in the drosophila larva body wall

複雑なセル形状の違いが達成されるかを理解するために、それは正確に微小管の組織に従うことが重要です。 ショウジョウバエ幼虫の体壁は、細胞や組織の形態形成を研究するモデルであるいくつかの細胞型を含んでいます。例えば、気管はチューブの形態形成1、 ショウジョウバエの幼虫の感覚ニューロンは、一般の解明と樹状突起分化2-5と変性6のニューロンクラス特有のメカニズムを、プライマリシステムとなっている樹状分枝（DA）を調べるために使用されます。 。 樹状突起の枝の形状は、ニューロンのクラス間、そしてさらに、単一ニューロン7,8の異なるブランチ間で著しく異なることがあります。 DAニューロンにおける遺伝学的研究では、差動細胞骨格の組織が ​​樹枝形状4,9-11の形態学的相違の根底にあることを示唆している。我々は、堅牢な免疫標識法を提供するDA感覚ニューロン樹状突起（図1、2、動画1） の in vivo微小管の組織で SSAY。このプロトコルは、最初の幼虫の解剖と免疫染色、アクティブな感覚ニューロンの樹状突起の伸長と分岐組織は12,13を発生している段階を示しています。 感覚ニューロンを染色することに加えて、この方法では、堅牢な筋肉の微小管の組織のラベリング（作品2本、3）、気管（図3、ムービー3）、およびその他の体壁の組織を実現しています。それは、メカニズムをコントロールする組織および細胞の形状を調査するときに体壁のin situで微小管の組織を分析することを希望する研究者のために貴重です。

Watch this Scientific Journal Video about Immunohistological Labeling of Microtubules in Sensory Neuron Dendrites, Tracheae, and Muscles in the Drosophila Larva Body Wall at JoVE.com

Immunohistological Labeling of Microtubules in Sensory Neuron Dendrites, Tracheae, and Muscles in the Drosophila Larva Body Wall

このようなニューロンの樹状突起のような複雑な細胞の形状は、開発中に達成されるかを理解するために、それは正確にアッセイの微小管の組織にできることが重要です。ここでは、樹状突起分枝のニューロン感覚樹状突起の微小管の組織、気管、筋肉を調べるために堅牢な免疫標識法を説明し、他の<em&gt;ショウジョウバエ</em&gt;幼虫の体壁の組織。

免疫組織学的な感覚ニューロンの樹状突起における微小管の標識、気管、との筋肉ショウジョウバエ幼虫の体壁

To understand how differences in complex cell shapes are achieved, it is important to accurately follow microtubule organization. The Drosophila larval ...

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Neuroscience

Immunohistological Labeling of Microtubules in Sensory Neuron Dendrites, Tracheae, and Muscles in the Drosophila Larva Body Wall

Live-cell Imaging of Sensory Organ Precursor Cells in Intact Drosophila Pupae

Live-cell Imaging of Sensory Organ Precursor Cells in Intact Drosophila Pupae

無傷の感覚器官の前駆細胞の生細胞イメージングショウジョウバエ蛹

Since the discovery of Green Fluorescent Protein (GFP), there has been a revolutionary change in the use of live-cell imaging as a tool for understanding ...

Morphological Analysis of Drosophila Larval Peripheral Sensory Neuron Dendrites and Axons Using Genetic Mosaics

Morphological Analysis of Drosophila Larval Peripheral Sensory Neuron Dendrites and Axons Using Genetic Mosaics

の形態素解析ショウジョウバエ</em遺伝モザイクを使用>幼虫末梢感覚ニューロンの樹状突起と軸索

Nervous system development requires the correct specification of neuron position and identity, followed by accurate neuron class-specific dendritic ...

Visualization of Proprioceptors in Drosophila Larvae and Pupae

Visualization of Proprioceptors in Drosophila Larvae and Pupae

でProprioceptorsの可視化<em&gt;ショウジョウバエ</em&gt;幼虫および蛹

Proprioception is the ability to sense the motion, or position, of body parts by responding to stimuli arising within the body. In fruitflies and other ...

Retrograde Fluorescent Labeling Allows for Targeted Extracellular Single-unit Recording from Identified Neurons In vivo

Retrograde Fluorescent Labeling Allows for Targeted Extracellular Single-unit Recording from Identified Neurons In vivo

逆行性蛍光標識は、特定された神経細胞から標的細胞外シングルユニット記録を可能にします<em生体内で&gt;</em

The overall goal of this method is to record single-unit responses from an identified population of neurons. In vivo electrophysiological recordings from ...

Labeling of Single Cells in the Central Nervous System of Drosophila melanogaster

Labeling of Single Cells in the Central Nervous System of Drosophila melanogaster

の中枢神経系における単一細胞のラベリング<em&gt;キイロショウジョウバエ</em

In this  article we describe how to individually label neurons in the embryonic CNS of Drosophila melanogaster by juxtacellular injection of the ...

Whole Mount Immunolabeling of Olfactory Receptor Neurons in the Drosophila Antenna

Whole Mount Immunolabeling of Olfactory Receptor Neurons in the Drosophila Antenna

における嗅覚受容ニューロンのホールマウント免疫標識<em&gt;ショウジョウバエ</em&gt;アンテナ

Odorant molecules bind to their target receptors in a precise and coordinated manner. Each receptor recognizes a specific signal and relays this ...

Olfactory Behaviors Assayed by Computer Tracking Of Drosophila in a Four-quadrant Olfactometer

Olfactory Behaviors Assayed by Computer Tracking Of Drosophila in a Four-quadrant Olfactometer

のコンピュータ追跡することによってアッセイ嗅覚行動<em&gt;ショウジョウバエ</em&gt; 4象限オルファクトメーターで

A key challenge in neurobiology is to understand how neural circuits function to guide appropriate animal behaviors. Drosophila melanogaster is an ...

Labeling and Imaging of Amyloid Plaques in Brain Tissue Using the Natural Polyphenol Curcumin

天然ポリフェノールクルクミンを用いた脳組織におけるアミロイドプラークの標識とイメージング

Deposition of amyloid beta protein (A&#946;) in extra- and intracellular spaces is one of the hallmark pathologies of Alzheimer&#39;s disease (AD). ...

Ballistic Labeling of Pyramidal Neurons in Brain Slices and in Primary Cell Culture

脳スライスと一次細胞培養における錐体ニューロンの弾道標識

It has been reported that the size and shape of dendritic spines is related to their structural plasticity. To identify the morphological structure of ...

An Emerging Target Paradigm to Evoke Fast Visuomotor Responses on Human Upper Limb Muscles

人間の上肢筋肉に対する高速ビスオモター応答を呼び起こす新たな標的パラダイム

To reach towards a seen object, visual information has to be transformed into motor commands. Visual information such as the object&#8217;s color, shape, ...