Name: human internal mammary artery (ima) transplantation and stenting: a human model to study the development of in-stent restenosis
Uploaded: 2012-05-09T12:00:00
Description: Watch this Scientific Journal Video about Human Internal Mammary Artery IMA Transplantation and Stenting: A Human Model to Study the Development of In-Stent Restenosis at JoVE.com

作者感谢她的贡献：克里斯蒂安Pahrmann。特别感谢爱惜康，Norderstedt的，汉堡（德国）提供的缝合材料。 资金宋佳Schrepfer已收到来自德意志研究联合会（DFG）（SCHR992 / 3 1 SCHR992/4-1）研究补助金。这项工作是由ISHLT沙姆韦事业发展基金2010年和福尔克研究经费（斯坦福大学）的支持。

1。乳内动脉（IMA）准备<ol><li>动脉内皮剥脱2法国福格蒂动脉取栓导管（百特医疗用品，迪尔菲尔德，IL，USA）的通过。拉导管贯穿整个船舶长度的两倍，以确保内皮损伤。 </li><li>使用的长度为8mm和2.5毫米，直径3毫米（如Translumina，育空支架）的任何人的支架。注意：支架的直径应不超过10％以上的血管直径，以避免前，后支架狭窄。注意：请不要在同一研究中切换支架的长度。 </li><li>使用合适...

<ol>
	<li>Deuse, T., Ikeno, F., Robbins, R. C., Schrepfer, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Imaging+In-Stent+Restenosis:+An+Inexpensive,+Reliable,+and+Rapid+Preclinical+Model.">Imaging In-Stent Restenosis: An Inexpensive, Reliable, and Rapid Preclinical Model.</a> J. Vis. Exp. (31), e1346 (2009).</li><li>Oyamada, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Trans-iliac+rat+aorta+stenting:+a+novel+high+throughput+preclinical+stent+model+for+restenosis+and+thrombosis.">Trans-iliac rat aorta stenting: a novel high throughput preclinical stent model for restenosis and thrombosis.</a> J. Surg. Res. 166, e91-e95 (2011).</li><li>Chamberlain, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+novel+mouse+model+of+in+situ+stenting.">A novel mouse model of in situ stenting.</a> Cardiovascular research. 85, 38-44 (2010).</li><li>Deuse, T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Introducing+the+first+polymer-free+leflunomide+eluting+stent.">Introducing the first polymer-free leflunomide eluting stent.</a> Atherosclerosis. 200, 126-134 (2008).</li><li>Finn, A. V. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Differential+healing+after+sirolimus,+paclitaxel,+and+bare+metal+stent+placement+in+combination+with+peroxisome+proliferator-activator+receptor+gamma+agonists:+requirement+for+mTOR/Akt2+in+PPARgamma+activation.">Differential healing after sirolimus, paclitaxel, and bare metal stent placement in combination with peroxisome proliferator-activator receptor gamma agonists: requirement for mTOR/Akt2 in PPARgamma activation.</a> Circulation research. 105, 1003-1012 (2009).</li><li>Tellez, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Coronary+bare+metal+stent+implantation+in+homozygous+LDL+receptor+deficient+swine+induces+a+neointimal+formation+pattern+similar+to+humans.">Coronary bare metal stent implantation in homozygous LDL receptor deficient swine induces a neointimal formation pattern similar to humans.</a> Atherosclerosis. 213, 518-524 (2010).</li><li>Suzuki, Y., Yeung, A. C., Ikeno, F. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+pre-clinical+animal+model+in+the+translational+research+of+interventional+cardiology.">The pre-clinical animal model in the translational research of interventional cardiology.</a> JACC Cardiovasc. Interv. 2, 373-383 (2009).</li><li>Holt, C. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Intimal+proliferation+in+an+organ+culture+of+human+internal+mammary+artery.">Intimal proliferation in an organ culture of human internal mammary artery.</a> Cardiovascular research. 26, 1189-1194 (1992).</li><li>Swanson, N., Javed, Q., Hogrefe, K., Gershlick, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Human+internal+mammary+artery+organ+culture+model+of+coronary+stenting:+a+novel+investigation+of+smooth+muscle+cell+response+to+drug-eluting+stents.">Human internal mammary artery organ culture model of coronary stenting: a novel investigation of smooth muscle cell response to drug-eluting stents.</a> Clin. Sci. (Lond). 103, 347-353 (2002).</li></ol>

虽然在体内研究模型不同的是现有的调查支架植入后内膜增生的发展，这些车型仍然面临着转化的障碍需要克服。此外，大量的动物模型是昂贵和特殊的住房条件以及手术设备是不是所有实验室。 使用人类的IMA研究人类的内膜增生及支架内再狭窄的发展进行了研究之前， 当然在器官培养8,9 原位 。新的人性化的IMA模型构成了翻译的办法来解决支架...

<table border="1"><tbody><tr><td> 试剂名称 </td><td> 公司 </td><td> 目录编号 </td><td> 评论 </td></tr><tr><td> 2法国Fogarty导管</td><td>迪尔菲尔德，IL，美国百特医疗用品， </td><td> 120602F </td><td></td></tr><tr><td>育空支架</td><td> translumina有限公司，Hechingen，德国</td><td></td><td>根据您的研究方案，使用您所选择的支架</td></tr><tr><td>的RPMI媒体</td><td> biochrom </td><td> Nr.F1275 </td><td></td></tr><tr><td>肝素</td><td>巴克斯特</td><td> 2B0953 </td><td></td></tr><tr><td>异氟醚</td><td>住持</td><td> B506 </td><td></td></tr><tr><td>普罗沃碘</td><td>优碘Puredue医药</td><td>条形码：5995327165830 </td><td></td></tr><tr><td> 80％ethano升</td><td>赫耶尔</td><td>教育电视80/0500 </td><td></td></tr><tr><td>微型钳</td><td>哈佛仪器</td><td> PY2-61-0186 </td><td></td></tr><tr><td>缝线8-0 </td><td>强生公司</td><td> 2808G </td><td></td></tr><tr><td>缝线6-0 </td><td>强生公司</td><td> 1698Ĥ </td><td></td></tr><tr><td>卡洛芬</td><td> feizer兽医</td><td> PZN含量：0110208 </td><td></td></tr><tr><td>安乃近</td><td> Ratiopharm公司</td><td></td><td></td></tr><tr><td>目标检索解决方案，PH9 </td><td> Dako公司</td><td> S2368 </td><td></td></tr><tr><td>图像FX信号增强</td><td> Invitrogen公司</td><td> I36933 </td><td></td></tr><tr><td>鼠单克隆抗GFP抗体</td><td> BD的生活色彩</td><td> 632381 </td><td></td></tr><tr><td>初级抗体稀释剂</td><td> Dako公司</td><td> S3022 </td><td></td></tr><tr><td>的Alexa Fluor 488山羊抗鼠IgG </td><td> Invitrogen公司</td><td> A11017 </td><td></td></tr><tr><td>二次抗体稀释剂</td><td> Dako公司</td><td> S0809 </td><td></td></tr><tr><td>兔polycolonal抗平滑肌α-肌动蛋白</td><td> abcam， </td><td> ab5694 </td><td></td></tr><tr><td>的Alexa Fluor 555山羊抗兔IgG </td><td> Invitrogen公司</td><td> A21430 </td><td></td></tr><tr><td>延长黄金的抗淬灭剂</td><td> Invitrogen公司</td><td> P36930 </td><td></td></tr></tbody></table>

human internal mammary artery (ima) transplantation and stenting: a human model to study the development of in-stent restenosis

体内研究模型的临床前研究后内膜增生的血管内支架置入术的发展中的病理生物学和病理生理过程转化为1,2的方法是至关重要的。 常用的动物模型包括小鼠，大鼠，兔，猪3-5。然而，进入临床的设置，这些模型的翻译仍然困难，因为这些生物的进程已经开始研究在动物血管，但以前从未在人类研究模型6,7。在这部影片中，我们展示了一个新的人性化的模型，以克服这种转化的差距。所示的过程，重现性好，操作方便，快速执行，并适合学习内膜增生的发展和多样化支架的适用性。 该视频演示了如何执行，通过移植到免疫缺陷大鼠的血管支架技术，标识作为人类的起源细胞增殖。

Watch this Scientific Journal Video about Human Internal Mammary Artery IMA Transplantation and Stenting: A Human Model to Study the Development of In-Stent Restenosis at JoVE.com

Human Internal Mammary Artery IMA Transplantation and Stenting: A Human Model to Study the Development of In-Stent Restenosis

这个视频显示了一个模型来研究支架使用在人类免疫缺陷鼠模型的船只（IMA）的部署后内膜增生的发展。

人乳内动脉（IMA）移植及内支架置入术：人体模型支架内再狭窄的研究开发

Preclinical in vivo research models to investigate pathobiological and pathophysiological processes in the development of intimal hyperplasia after vessel ...

Research

JoVE Journal

Medicine

Human Internal Mammary Artery (IMA) Transplantation and Stenting: A Human Model to Study the Development of In-Stent Restenosis

Orthotopic Aortic Transplantation: A Rat Model to Study the Development of Chronic Vasculopathy

主动脉原位移植大鼠模型，研究慢性血管病变的发展

Research models of chronic rejection are essential to investigate pathobiological and pathophysiological processes during the development of transplant ...

科研

医学

Utilizing Transcranial Magnetic Stimulation to Study the Human Neuromuscular System

利用经颅磁刺激，以研究人类的神经肌肉系统

Transcranial magnetic stimulation (TMS) has been in use for more than 20 years 1, and has grown exponentially in popularity over the past decade.  While ...

Stem Cell Transplantation in an in vitro Simulated Ischemia/Reperfusion Model

Stem Cell Transplantation in an in vitro Simulated Ischemia/Reperfusion Model

在干细胞移植在体外模拟缺血/再灌注模型

Stem cell transplantation protocols are finding their way into clinical practice1,2,3. Getting better results, making the protocols more robust, and ...

Human Neuroendocrine Tumor Cell Lines as a Three-Dimensional Model for the Study of Human Neuroendocrine Tumor Therapy

人类神经内分泌肿瘤细胞株为研究人类神经内分泌肿瘤治疗的三维模型

Neuroendocrine tumors (NETs) are rare tumors, with an incidence of two per 100,&#x200A;000 individuals per year, and they account for 0.5% of all human ...

A Murine Model of Stent Implantation in the Carotid Artery for the Study of Restenosis

颈动脉支架植入再狭窄的研究的小鼠模型

Despite  the considerable progress made in the stent development in the last decades,  cardiovascular diseases remain the main cause of death in western ...

A Human Ex Vivo Atherosclerotic Plaque Model to Study Lesion Biology

A Human Ex Vivo Atherosclerotic Plaque Model to Study Lesion Biology

一个人力<em&gt;前体内</em&gt;动脉粥样硬化斑块模型研究病变的生物学

Atherosclerosis is a chronic inflammatory disease of the vasculature. There are various methods to study the inflammatory compound in atherosclerotic ...

Excitotoxic Stimulation of Brain Microslices as an In vitro Model of Stroke

Excitotoxic Stimulation of Brain Microslices as an In vitro Model of Stroke

脑Microslices的兴奋性刺激作为<em&gt;在体外</em&gt;中风模型

Examining molecular mechanisms involved in neuropathological conditions, such as ischemic stroke, can be difficult when using whole animal systems. As ...

Murine Corneal Transplantation: A Model to Study the Most Common Form of Solid Organ Transplantation

小鼠角膜移植模型，研究实体器官移植的最常见形式

Corneal transplantation is the most common form of organ transplantation in the United States with between 45,000 and 55,000 procedures performed each ...

Establishment of a Human Multiple Myeloma Xenograft Model in the Chicken to Study Tumor Growth, Invasion and Angiogenesis

建立一个人类多发性骨髓瘤异种移植模型中的鸡，研究肿瘤的生长，侵袭和血管生成

Multiple myeloma (MM), a malignant plasma cell disease, remains incurable and novel drugs are required to improve the prognosis of patients. Due to the ...

Encapsulation Thermogenic Preadipocytes for Transplantation into Adipose Tissue Depots

封装产热前脂肪细胞移植到脂肪组织仓库

Cell encapsulation was developed to entrap viable cells within semi-permeable membranes. The engrafted encapsulated cells can exchange low molecular ...