Stanford University School of Medicine

9 ARTICLES PUBLISHED IN JoVE

Biology

Collecting and Measuring Nociceptive and Inflammatory Mediators in Surgical Wounds

Brendan Carvalho¹, David J. Clark¹, David Yeomans¹, Martin S. Angst¹

¹Department of Anesthesiology, Stanford University School of Medicine

A technique to collect and measure surgical wound biochemical mediators at specific time points.

Biology

Human In-Vivo Bioassay for the Tissue-Specific Measurement of Nociceptive and Inflammatory Mediators

Martin S Angst¹, Martha Tingle¹, Martin Schmelz^2,3, Brendan Carvalho¹, David C Yeomans¹

¹Department of Anesthesia, Stanford University School of Medicine, ²Department of Anaesthesiology, University of Mannheim, ³Department of Anaesthesiology, University of Heidelberg

A technique is presented for the in-vivo collection of interstitial fluid samples from pertinent tissue sites (here, experimentally inflamed skin) for the measurement of biochemicals mediating pain and inflammation.

Biology

Determining heat and mechanical pain threshold in inflamed skin of human subjects

Martin S Angst¹, Martha Tingle¹, Nicholas G Phillips¹, Brendan Carvalho¹

¹Department of Anesthesia, Stanford University School of Medicine

Algorithms assessing heat and mechanical pain thresholds in experimentally inflamed skin of human study subjects are shown. The two pain testing paradigms independently examine nociceptive processing by the two major peripheral nerve fiber populations transmitting pain, i.e., non-myelinated C fibers and small myelinated A-delta fibers.

Medicine

An Experimental Paradigm for the Prediction of Post-Operative Pain (PPOP)

Ruth Landau¹, John C. Kraft¹, Lisa Y. Flint¹, Brendan Carvalho¹, Philippe Richebé¹, Monica Cardoso¹, Patricia Lavand'homme¹, Michal Granot¹, David Yarnitsky¹, Alex Cahana¹

¹Department of Anesthesiology and Pain Medicine, University of Washington School of Medicine

Diffuse noxious inhibitory control, temporal summation and wound hyperalgesia testing are demonstrated in the obstetric patient. These tests evaluate inhibitory and excitatory mechanisms of pain processing and are here utilized to evaluate endogenous analgesia at different time-points during pregnancy and the peripartum period to help reveal individual s risk for persistent pain.

Medicine

Human Internal Mammary Artery (IMA) Transplantation and Stenting: A Human Model to Study the Development of In-Stent Restenosis

Xiaoqin Hua^1,2, Tobias Deuse^1,2, Evangelos D. Michelakis³, Alois Haromy³, Phil S. Tsao⁴, Lars Maegdefessel⁴, Reinhold G. Erben⁵, Claudia Bergow⁵, Boris B. Behnisch⁶, Hermann Reichenspurner^1,2, Robert C. Robbins⁷, Sonja Schrepfer^1,2,7

¹University Heart Center Hamburg, TSI-Lab, Germany, ²Cardiovascular Research Center, University of Hamburg, ³Department of Medicine, Cardiology Division, Pulmonary Hypertension Program, University of Alberta, ⁴Department of Medicine, Stanford University School of Medicine , ⁵Department of Biomedical Sciences, Institute of Physiology, Pathophysiology, and Biophysics, University of Veterinary Medicine, Vienna, ⁶Translumina GmbH, Hechingen, ⁷Department of Cardiothoracic Surgery, Stanford University School of Medicine

This video shows a model to study the development of intimal hyperplasia after stent deployment using a human vessel (IMA) in an immunodeficient rat model.

Biology

Rapid Genetic Analysis of Epithelial-Mesenchymal Signaling During Hair Regeneration

Wei-Meng Woo^*1, Scott X. Atwood^*1, Hanson H. Zhen¹, Anthony E. Oro¹

¹Program in Epithelial Biology, Stanford University School of Medicine

Tissue-specific analysis of a hair follicle regeneration model using lentivirus to mediate gain- or loss-of-function.

Medicine

Probe-based Confocal Laser Endomicroscopy of the Urinary Tract: The Technique

Timothy C. Chang^1,2, Jen-Jane Liu^1,2, Joseph C. Liao^1,2

¹Department of Urology, Stanford University School of Medicine , ²Veterans Affairs Palo Alto Health Care System

Probe-based confocal laser endomicroscopy enables real-time microscopy of the human urinary tract during cystoscopy, providing dynamic, intravital imaging of pathological states such as bladder cancer with cellular resolution. Endomicroscopy may augment the diagnostic accuracy of standard white light endoscopy and provide intraoperative image guidance to improve surgical resection.

Medicine

Collecting And Measuring Wound Exudate Biochemical Mediators In Surgical Wounds

Brendan Carvalho¹, David J Clark¹, David Yeomans¹, Martin S Angst¹

¹Department of Anesthesia, Stanford University School of Medicine

This article provides a detailed and visual description of a methodology for collecting and measuring biochemical inflammatory and nociceptive mediators at the surgical wound site following cesarean delivery. This human bioassay has been used to determine correlations between wound and serum cytokine concentrations and drug-mediated changes in wound cytokines, chemokines and neuropetides.

Medicine

Inducing Myointimal Hyperplasia Versus Atherosclerosis in Mice: An Introduction of Two Valid Models

Mandy Stubbendorff^*1,2, Xiaoqin Hua^*1,2, Tobias Deuse^1,2,3, Ziad Ali^4,5, Hermann Reichenspurner^2,3, Lars Maegdefessel⁶, Robert C. Robbins⁷, Sonja Schrepfer^1,2,3,4

¹Transplant and Stem Cell Immunobiology Lab, Cardiovascular Research Center, University Hospital Hamburg, ²Cardiovascular Research Center (CVRC) and DZHK University Hamburg, ³Department of Cardiovascular Surgery, University Heart Center Hamburg, ⁴Center for Interventional Vascular Therapy, Division of Cardiology, Columbia University, ⁵Cardiovascular Research Foundation, New York, ⁶Karolinska Institute, Stockholm, ⁷Department of Cardiothoracic Surgery, Stanford University School of Medicine, Falk Cardiovascular Research Center

This video shows two models of intimal plaque development in murine arteries and emphasizes the differences in myointimal hyperplasia and atherosclerosis.