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DOI :
10.3791/3669-v
•
7:39 min
June 29th, 2012
Chapters
0:05
Title
1:20
Perfusion of the Liver
3:31
Preparation of the Biliary Tree and Isolation of the Portal Fibroblast Fraction
6:42
Results: Representative Primary Portal Fibroblasts Isolated from Adult Rat Liver
7:21
Conclusion
一个从大鼠肝门成纤维细胞的分离技术。肝脏被灌注和消化原位胶原酶,其次是体外消化肝细胞浆和大小选择。这种方法提供了一个门户网站的成纤维细胞的纯人口,而不需要通过文化。
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