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DOI :
10.3791/3669-v
•
7:39 min
June 29th, 2012
Chapters
0:05
Title
1:20
Perfusion of the Liver
3:31
Preparation of the Biliary Tree and Isolation of the Portal Fibroblast Fraction
6:42
Results: Representative Primary Portal Fibroblasts Isolated from Adult Rat Liver
7:21
Conclusion
쥐의 간장에서 포털 섬유아 세포를 분리를위한 기법을 설명합니다. 간은는 perfused과 소화하고 현장에서 collagenase으로 이어 예 생체내 소화. 이 방법은 문화의 통과를 위해 필요없이 포털 섬유아 세포의 순수 인구를 제공합니다.
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