Name: epidural intracranial pressure measurement in rats using a fiber-optic pressure transducer
Uploaded: 2012-04-25T12:00:00
Description: Watch this Scientific Journal Video about Epidural Intracranial Pressure Measurement in Rats Using a Fiber-optic Pressure Transducer at JoVE.com

这个项目是由国家中风基金会，亨特医学研究所（HMRI）和国家健康与医学研究理事会（NH＆MRC的），澳大利亚。特别感谢卫生车间员工在纽卡斯尔大学的教师，他们的技术专长。

1。头骨穿透<ol><li>麻醉与异氟醚（5％的诱导，维护1.5-2％）在70％N2和30％O 2的老鼠。麻醉诱导后，将大鼠俯卧变暖板，老鼠的鼻子在麻醉鼻锥定位。 </li><li>虽然维持麻醉，固定在头部立体定向框架，插入耳酒吧，直到头部稳定。确保呼吸不受损害。 （ 图2 A）。 </li><li>头皮皮下注入持久的局部麻醉，布比卡因0.3毫升0.5％（辉瑞公司，澳大利亚），然后才作出一个1.5...

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这里介绍的过程，使颅内压非常灵敏，准确的记录。这种微创技术，避免了显着的脑外伤，定位在硬膜外腔压力传感器和脑组织或心室。 的关键步骤包括：1）通过头骨钻 - 护理必须采取不刺破硬脊膜或损害脑组织的基本; 2）确保紧的密封嵌缝材料 - 如果有任何泄漏，ICP跟踪将不会可靠。当适当的位置，ICP传感器读数将不仅ICP备准确跟踪，但呼吸和心率。与灵感，更负面的胸?...

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epidural intracranial pressure measurement in rats using a fiber-optic pressure transducer

升高颅内压（ICP）是一个重大的问题，在缺血性脑损伤的几种形式，包括中风，脑外伤和心脏骤停。这个海拔可能导致进一步的神经损伤，小脑幕1,2,3,4症，脑压，神经赤字或增加脑梗塞2,4的 ​​形式。目前的治疗方法往往不足以控制在临床上设置5,6,7 ICP升高。因此，有一个准确的ICP测量方法，在动物模型中需要进一步我们的基本机制的理解和发展新的治疗方法为ICP升高。 在临床和实验设置ICP备无法估计没有直接测量。目前存在的几种方法ICP导管插入。这些脑室导管已成为临床上的“金标准”的ICP测量人类8。这种方法INVolves颅骨部分切除和导管通过脑组织的仪器。因此，脑室导管感染发生率6-11％9。出于这个原因，硬膜下和硬膜外cannulations的缺血性损伤的动物模型已成为首选方法。 各种ICP测量技术已被改编为动物模型，其中，充满液体的的遥测导管10和固态导管是最常用的11,12,13,14,15。充液系统开发行中的气泡，很容易造成假ICP备读数。固态探头避免此问题（ 图1）。另外一个问题是没有根据头骨或装修成心室导管造成脑损伤，可能会改变实验结果。因此，我们已经开发出一种方法，连续硬膜外腔放置一个ICP导管，但避免了NE插入颅骨和大脑之间。 一个光纤压力导管（420LP，SAMBA传感器，瑞典），用来衡量在硬膜外位置ICP因为压力传感器（导管很尖）的位置被发现产生在这个模型中的信号高保真ICP 。有其他厂家类似的光纤技术，可与我们的方法使用的13。替代固态导管，导管尖端一侧有压力传感器，将不适合这种模式的信号将挫伤由监测螺丝的存在。 在这里，我们提出了一个相对简单和准确的方法来衡量ICP备。这种方法可用于广泛的跨ICP备相关的动物模型。

Watch this Scientific Journal Video about Epidural Intracranial Pressure Measurement in Rats Using a Fiber-optic Pressure Transducer at JoVE.com

Epidural Intracranial Pressure Measurement in Rats Using a Fiber-optic Pressure Transducer

介绍一种新的技术来记录颅骨内的压力。微创的方法是使用一个光纤压力传感系统，准确地测量不会造成重大的脑外伤颅内压（ICP）在麻醉大鼠。该技术可以用在广泛的实验模型。

硬膜外颅内压测量中使用的光纤压力传感器的大鼠

Elevated intracranial pressure (ICP) is a significant problem in several forms of ischemic brain injury including stroke, traumatic brain injury and ...

Research

JoVE Journal

Medicine

Pressure Controlled Ventilation to Induce Acute Lung Injury in Mice

压力控制通气诱导小鼠急性肺损伤

Murine models are extensively used to investigate acute injuries of different organs systems (1-34). Acute lung injury (ALI), which occurs with prolonged ...

科研

医学

Automated Midline Shift and Intracranial Pressure Estimation based on Brain CT Images

根据脑CT图像的自动中线移位和颅内压的估计

In this paper we present an automated system based mainly on the computed tomography (CT) images consisting of two main components: the midline shift ...

Ascending Aortic Constriction in Rats for Creation of Pressure Overload Cardiac Hypertrophy Model

大鼠建立压力超负荷心肌肥厚模型在升主动脉缩窄

Ascending aortic constriction is the most common and successful surgical model for creating pressure overload induced cardiac hypertrophy and heart ...

Assessing Myogenic Response and Vasoactivity In Resistance Mesenteric Arteries Using Pressure Myography

评估肌反应及血管活性阻力肠系膜动脉使用压力肌动描记

Small resistance arteries constrict and dilate respectively in response to increased or decreased intraluminal pressure; this phenomenon known as myogenic ...

Measurement of the Pressure-volume Curve in Mouse Lungs

In recent decades the mouse has become the primary animal model of a variety of lung diseases. In models of emphysema or fibrosis, the essential ...

Intrauterine Telemetry to Measure Mouse Contractile Pressure In Vivo

Intrauterine Telemetry to Measure Mouse Contractile Pressure In Vivo

宫内遥测来衡量鼠标收缩压力<em&gt;在体内</em

A complex integration of molecular and electrical signals is needed to transform a quiescent uterus into a contractile organ at the end of pregnancy. ...

Trabecular Meshwork Response to Pressure Elevation in the Living Human Eye

小梁响应压升高在活的人类眼睛

The mechanical characteristics of the trabecular meshwork (TM) are linked to outflow resistance and intraocular pressure (IOP) regulation. The rationale ...

Long-term Blood Pressure Measurement in Freely Moving Mice Using Telemetry

自由活动小鼠使用遥测长期血压测量

During the development of new vasoactive agents, arterial blood pressure monitoring is crucial for evaluating the efficacy of the new proposed drugs. ...

Spatial Measurements of Perfusion, Interstitial Fluid Pressure and Liposomes Accumulation in Solid Tumors

灌注的空间测量，实体肿瘤间质流体压力和脂质体积累

The heterogeneous intra-tumoral accumulation of liposomes is a critical determinant of their efficacy. Both the chaotic tumor microcirculation and ...

Cavernous Nerve Stimulation and Recording of Intracavernous Pressure in a Rat

大鼠海绵状神经刺激与海绵压力记录

The stimulation of the cavernous nerve (CN) and measurement of intracavernous pressure (ICP) have been used extensively to test and evaluate therapies for ...