Name: epidural intracranial pressure measurement in rats using a fiber-optic pressure transducer
Uploaded: 2012-04-25T12:00:00
Description: Watch this Scientific Journal Video about Epidural Intracranial Pressure Measurement in Rats Using a Fiber-optic Pressure Transducer at JoVE.com

Dette projekt blev finansieret af National Stroke Foundation, Hunter Medical Research Institute (HMRI) og National Sundhed og Medical Research Council (NH &amp; MRC), Australien. Særligt tak til SUND Workshop ansatte ved University of Newcastle for deres tekniske ekspertise.

1. Skull Penetration <ol><li> Bedøve rotte med isofluran (5% induktion, 1,5-2% vedligeholdelse) i 70% N2 og 30% O2. Efter induktion af anæstesi, rotte tilbøjelig på en varmeplade placere, positionere rottens næsen i en anæstetisk næsekegle. </li><li> Samtidig med at anæstesi, sikre hovedet i en stereotaktisk ramme, indsætte øre-barer, indtil hovedet er stabiliseret. Sørg for, åndedrættet bliver ikke nedsat. (Figur 2-A). </li><li> Injicér hovedbund subkutant med lang...

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Fremgangsmåden fremlagt her, muliggør en meget følsom og nøjagtig registrering af intrakranielt tryk. Denne minimalt invasiv teknik undgår væsentlig hjernetraume ved at placere trykføleren i det epidurale rum og ikke hjernevæv eller ventriklerne. De kritiske trin omfatter: 1) boring gennem kraniet - man skal passe ikke at gennembore dura eller skade underliggende hjernevæv, 2) at sikre en tæt forsegling med tætningsmateriale - hvis der er nogen lækage, vil ICP spor ikke pålideli...

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epidural intracranial pressure measurement in rats using a fiber-optic pressure transducer

Forhøjet intrakranielt tryk (ICP) er et stort problem i mange former for iskæmisk hjerneskade, herunder slagtilfælde, traumatisk hjerneskade og hjertestop. Denne stigning kan resultere i yderligere neurologisk skade, i form af transtentorial brok 1,2,3,4, midthjernen kompression, neurologisk deficit eller øget cerebral infarkt 2,4. Nuværende terapier er ofte utilstrækkelig til at kontrollere forhøjet ICP i kliniske omgivelser 5,6,7. Der er således et behov for nøjagtige fremgangsmåder til ICP måling i dyremodeller for at fremme forståelsen af ​​de grundlæggende mekanismer og udvikle nye behandlinger for forhøjet ICP. I både klinisk og eksperimentel indstilling ICP kan ikke vurderes uden direkte måling. Adskillige fremgangsmåder til ICP kateteret findes i dag. Af disse intraventrikulær kateteret er blevet klinisk &#39;gold standard &quot;for ICP måling i mennesker 8. Denne fremgangsmåde INVolves delvis fjernelse af kraniet og instrumentering af kateteret gennem hjernevæv. Følgelig intraventrikulære katetre har en infektion på 6-11% 9. Af denne grund har subduralt og epidural cannulations bliver de foretrukne fremgangsmåder i dyremodeller af iskæmisk skade. Forskellige ICP måleteknik er blevet tilpasset for dyremodeller, og af disse er væskefyldte telemetri katetre 10 og solid state katetre den hyppigst anvendte 11,12,13,14,15. De væskefyldte systemer er tilbøjelige til at udvikle luftbobler i linien, hvilket resulterer i falske ICP aflæsninger. Solid state prober undgå dette problem (figur 1). Et yderligere problem er montering af katetre under kraniet eller i ventriklerne uden at forårsage hjerneskade, der kan ændre de eksperimentelle resultater. Derfor har vi udviklet en fremgangsmåde, der placerer et ICP kateter sammenhængende med det epidurale rum, men undgår need for at indsætte den mellem kraniet og hjernen. En optisk fiber trykkateter (420LP, SAMBA sensorer, Sverige) blev anvendt til at måle ICP på det epidurale sted, fordi placeringen af ​​trykføleren (ved selve spidsen af ​​katetret), blev fundet at frembringe en high fidelity ICP signal i denne model . Der er andre producenter af lignende optiske fiber teknologi 13, som kan anvendes med vores metode. Alternative solid state katetre, der har trykføleren placeret ved siden af ​​kateterspidsen, ville ikke være passende til denne model, som det signal vil blive dæmpet ved tilstedeværelsen af ​​overvågningen skruen. Her præsenteres en relativt enkel og nøjagtig metode til måling ICP. Denne metode kan anvendes på tværs af en bred vifte af ICP-relaterede dyremodeller.

Watch this Scientific Journal Video about Epidural Intracranial Pressure Measurement in Rats Using a Fiber-optic Pressure Transducer at JoVE.com

Epidural Intracranial Pressure Measurement in Rats Using a Fiber-optic Pressure Transducer

En hidtil ukendt teknik til at registrere det tryk i kraniet er beskrevet. Den minimalt invasiv metode anvender et fiberoptisk trykfølende system til nøjagtigt at måle intrakranielle tryk (ICP) i bedøvede rotter uden at forårsage væsentlig hjernetraume. Teknikken kan anvendes i en lang række af eksperimentelle modeller.

Epidural intrakranielt tryk Måling i rotter med et fiberoptisk Pressure Transducer

Elevated intracranial pressure (ICP) is a significant problem in several forms of ischemic brain injury including stroke, traumatic brain injury and ...

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Medicine

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The stimulation of the cavernous nerve (CN) and measurement of intracavernous pressure (ICP) have been used extensively to test and evaluate therapies for ...