Name: epidural intracranial pressure measurement in rats using a fiber-optic pressure transducer
Uploaded: 2012-04-25T12:00:00
Description: Watch this Scientific Journal Video about Epidural Intracranial Pressure Measurement in Rats Using a Fiber-optic Pressure Transducer at JoVE.com

Этот проект финансируется Национальным фондом инсульта, Хантер Института медицинских исследований (HMRI) и Национального Здоровья и Медицинского исследовательского совета (NH и MRC), Австралия. Особая благодарность факультет Медицинский персонал семинар в Университете Ньюкасла своих технических знаний.

1. Череп Проникновение <ol><li> Анестезировать крыс с ИФ (5% индукции, 1,5-2% содержание) в 70% N 2 и 30% O 2. После вводного наркоза, поместите крысы ничком на потепление плиты, позиционирование нос крысы в ​​конус нос анестезии. </li><li> В то время как поддержание анестезии, обеспечение гол...

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Процедуры, представленные здесь дает очень чувствительный и точный учет внутричерепного давления. Это малоинвазивная методика позволяет избежать значительных травм мозга, поместив датчик давления в эпидуральном пространстве, а не ткани мозга или желудочков. Важные ш?...

<table border="1"><tbody><tr><td> Название реагента </td><td> Компания </td><td> Номер по каталогу </td><td> Комментарии </td></tr><tr><td> Стоматологическая мономера цемента </td><td> Генри Шайн </td><td> VX-SC500MLL </td><td></td></tr><tr><td> Стоматологическая Цемент полимерных </td><td> Генри Шайн </td><td> VX-SC1000GCL4 </td><td></td></tr><tr><td> Бормашины заусенцев размер 12 </td><td> Gunz стоматологические </td><td> EL104S001012/10 </td><td></td></tr><tr><td> Бормашины заусенцев размером 6 </td><td> Gunz стоматологические </td><td> EL104S001006/10 </td><td></td></tr><tr><td> Металлический винт </td><td> Магазине </td><td></td><td> 2 х 4 мм с шестигранной головкой. (Лабораторных изменение на 0,7 мм отверстие, просверленное через вал) </td></tr><tr><td> SAMBA Control Unit </td><td> Гарвардский аппарата </td><td> 50433102 </td><td></td></tr><tr><td> SAMBA датчика </td><td> Гарвардский аппарата </td><td> 50461122 </td><td> 420 LP, 15 см голого волокна, радио-непрозрачного покрытия </td></tr><tr><td> Silagum AV Моно уплотнительных материалов </td><td> Gunz стоматологические </td><td> RG 9152 </td><td> Vinylpolysiloxanes, водород полисилоксаны, наполнителей, пигментов, добавок, катализаторов plantinum </td></tr><tr><td> КГТО-A-Zyme </td><td> Алконокс, Inc </td><td> 1304 </td><td> Фермент-активные моющие средства порошкообразные </td></tr></tbody></table>

epidural intracranial pressure measurement in rats using a fiber-optic pressure transducer

Elevated intracranial pressure (ICP) is a significant problem in several forms of ischemic brain injury including stroke, traumatic brain injury and cardiac arrest. This elevation may result in further neurological injury, in the form of transtentorial herniation1,2,3,4, midbrain compression, neurological deficit or increased cerebral infarct2,4. Current therapies are often inadequate to control elevated ICP in the clinical setting5,6,7 . Thus there is a need for accurate methods of ICP measurement in animal models to further our understanding of the basic mechanisms and to develop new treatments for elevated ICP.
In both the clinical and experimental setting ICP cannot be estimated without direct measurement. Several methods of ICP catheter insertion currently exist. Of these the intraventricular catheter has become the clinical 'gold standard' of ICP measurement in humans8. This method involves the partial removal of skull and the instrumentation of the catheter through brain tissue. Consequently, intraventricular catheters have an infection rate of 6-11%9. For this reason, subdural and epidural cannulations have become the preferred methods in animal models of ischemic injury. 
Various ICP measurement techniques have been adapted for animal models, and of these, fluid-filled telemetry catheters10 and solid state catheters are the most frequently used11,12,13,14,15. The fluid-filled systems are prone to developing air bubbles in the line, resulting in false ICP readings. Solid state probes avoid this problem (Figure 1). An additional problem is fitting catheters under the skull or into the ventricles without causing any brain injury that might alter the experimental outcomes. Therefore, we have developed a method that places an ICP catheter contiguous with the epidural space, but avoids the need to insert it between skull and brain. 
An optic fibre pressure catheter (420LP, SAMBA Sensors, Sweden) was used to measure ICP at the epidural location because the location of the pressure sensor (at the very tip of the catheter) was found to produce a high fidelity ICP signal in this model. There are other manufacturers of similar optic fibre technologies13 that may be used with our methodology. Alternative solid state catheters, which have the pressure sensor located at the side of the catheter tip, would not be appropriate for this model as the signal would be dampened by the presence of the monitoring screw. 
Here, we present a relatively simple and accurate method to measure ICP. This method can be used across a wide range of ICP related animal models.

Watch this Scientific Journal Video about Epidural Intracranial Pressure Measurement in Rats Using a Fiber-optic Pressure Transducer at JoVE.com

Epidural Intracranial Pressure Measurement in Rats Using a Fiber-optic Pressure Transducer

Новый метод для записи давления внутри черепа описано. Малоинвазивных методов используются волоконно-оптические системы измерения давления для точного измерения внутричерепного давления (ВЧД) в анестезии крыс без причинения значительного травмы головного мозга. Методика может быть использована в широком диапазоне экспериментальных моделей.

Эпидуральная внутричерепного Измерение давления у крыс с помощью волоконно-оптических преобразователей давления

Elevated intracranial pressure (ICP) is a significant problem in several forms of ischemic brain injury including stroke, traumatic brain injury and ...

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Давление контролируемой вентиляции, чтобы вызвать острого повреждения легких у мышей

Murine models are extensively used to investigate acute injuries of different organs systems (1-34). Acute lung injury (ALI), which occurs with prolonged ...

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Автоматизированная Средняя линия сдвига и внутричерепных оценки давления на основе КТ головного мозга изображений

In this paper we present an automated system based mainly on the computed tomography (CT) images consisting of two main components: the midline shift ...

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Ascending aortic constriction is the most common and successful surgical model for creating pressure overload induced cardiac hypertrophy and heart ...

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Оценка Миогенный ответ и вазоактивности В Сопротивление брыжеечных артерий, используя миография давления

Small resistance arteries constrict and dilate respectively in response to increased or decreased intraluminal pressure; this phenomenon known as myogenic ...

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In recent decades the mouse has become the primary animal model of a variety of lung diseases. In models of emphysema or fibrosis, the essential ...

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Intrauterine Telemetry to Measure Mouse Contractile Pressure In Vivo

Внутриматочная телеметрии измерить мышь сократительной давления<em&gt; В Vivo</em

A complex integration of molecular and electrical signals is needed to transform a quiescent uterus into a contractile organ at the end of pregnancy. ...

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Трабекулярной сети Ответ на возвышенности давления в Живой человеческого глаза

The mechanical characteristics of the trabecular meshwork (TM) are linked to outflow resistance and intraocular pressure (IOP) regulation. The rationale ...

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Долгосрочный Измерение артериального давления у свободно передвигающихся мышей с использованием Телеметрия

During the development of new vasoactive agents, arterial blood pressure monitoring is crucial for evaluating the efficacy of the new proposed drugs. ...

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Пространственные измерения перфузии, интерстициальный давления жидкости и Липосомы Накопление в солидных опухолях

The heterogeneous intra-tumoral accumulation of liposomes is a critical determinant of their efficacy. Both the chaotic tumor microcirculation and ...

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Кавернозных нерва стимуляции и запись интракавернозные давления в крыса

The stimulation of the cavernous nerve (CN) and measurement of intracavernous pressure (ICP) have been used extensively to test and evaluate therapies for ...