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DOI :
10.3791/3698-v
March 16th, 2012
Chapters
0:05
Title
1:46
Production of Culture
2:53
Culture Preparation and Mixing (Mating)
4:27
Isolation of Trans-integrants
5:55
Results of Integrase Mediated Cassette Exchange (IMCE)
8:01
Conclusion
描述一个快速和有效的方法融入预制受体菌株,称为停机坪株,外源DNA的利益。该方法允许进入工程着陆垫一个给定的应变轨迹的站点特定的DNA盒的整合,通过共轭ΦC31整合表达。
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