Name: quantification of fungal colonization, sporogenesis, and production of mycotoxins using kernel bioassays
Uploaded: 2012-04-23T13:00:00
Description: Watch this Scientific Journal Video about Quantification of Fungal Colonization, Sporogenesis, and Production of Mycotoxins Using Kernel Bioassays at JoVE.com

我们要感谢他们的技术援助布兰登哈塞特和卡洛斯·奥尔蒂斯。这项工作是由国家科学基金会资助的IOB-0544428，内部监督办公室-0951272，的IOS-0925561博士迈克尔Kolomiets的支持，由美国农业部国家食品和农业研究所（粮农），AFRI植物育种和教育补助金＃2010-85117 -20539到博士。赛斯美利，托马斯Isakeit，和迈克尔Kolomiets的。

1。玉米内核生物活性的<ol><li>两个星期前，文化马铃薯葡萄糖琼脂（PDA）的病原真菌在28°C。 </li><li>选择具有类似的形状和大小的内核，最好是扁平的，所以他们打下水平的生物活性瓶的底部，并在50毫升猎鹰管。内核选择必须已同时在相同的环境，以确保类似种子的年龄和代谢产物的组成。 </li><li>晃动管在室温下用70％乙醇，用无菌水1分钟，10分钟，5分钟与6％次氯酸钠表面消毒内核。冲洗，...

<ol>
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<p class="jove_content"这里描述的方法已被广泛的测试和产生真菌孢子形成和霉菌毒素生产的量化结果被证明是强大的。此外，这些方法应该适用于从其他mycotoxigenic真菌（如花生，小麦，棉花，开心果等），很容易受到污染的植物物种的种子。主管植物病原菌互作分析，这是必须活着保存的种子。内核的胚胎方面的一个小伤口有利于感染，同时保持种子的活力。</p&gt;<p class="jove_content"有效运行的内核检测已被证明是来?...

<table border="1"><tbody><tr><td> 试剂名称 </td><td> 公司 </td><td> 产品编号 </td></tr><tr><td>马铃薯葡萄糖琼脂</td><td>费舍尔Scientifc </td><td> S71659A </td></tr><tr><td>吐温20 </td><td>费舍尔Scientifc </td><td> BP337-100 </td></tr><tr><td>塑料孵化容器</td><td> sterilite </td><td> 1713LAB06 </td></tr><tr><td>混合器</td><td> VICAM </td><td> 20200 </td></tr><tr><td> 24厘米凹槽滤纸</td><td> VICAM </td><td> 31240 </td></tr><tr><td> 1.5微米的玻璃微纤维</td><td> VICAM </td><td> 31955 </td></tr><tr><td>黄曲霉试验柱</td><td> VICAM </td><td> G1024 </td></tr><tr><td> afrla测试开发人员</td><td> VICAM </td><td> 32010 </td></tr><tr><td>甲醇</td><td> VICAM </td><td> 35016 </td></tr><tr><td>乙腈</td><td>费舍尔Scientifc </td><td> AC14952-0025 </td></tr><tr><td>乙醇</td><td>费舍尔王欧，吴文良TIFC </td><td> AC39769-0025 </td></tr><tr><td>的C-18固相萃取柱（PREP SEP SPE C18柱） </td><td>费舍尔Scientifc </td><td> 60108-304 </td></tr><tr><td>邻苯二甲醛（OPA）的</td><td>西格玛化工有限公司</td><td> 79760-5G </td></tr><tr><td>硼酸</td><td>费舍尔Scientifc </td><td> BP168-500 </td></tr><tr><td>四硼酸钠</td><td>费舍尔Scientifc </td><td> RDCS0330500 </td></tr><tr><td>巯基乙醇</td><td>费舍尔Scientifc </td><td> 45-000-231 </td></tr><tr><td>岛津高效液相色谱LC-20AT（泵） </td><td>岛津科学仪器公司</td><td> LC-20AT </td></tr><tr><td> ZORBAX ODS柱（4.6x150mm） </td><td>安捷伦科技公司</td><td> 443905-902 </td></tr><tr><td> shimatzu RF-10Axl荧光检测</td><td>岛津科学仪器公司</td><td> RF-10AXL </td></tr><tr><td>磷酸钠</td><td>费舍尔Scientifc </td><td> AC38987-0010 </td></tr><tr><td> FB1的标准</td><td> Sigma公司</td><td> F1147-1毫克</td></tr><tr><td>氯仿</td><td>厂商VWR </td><td> MK444410 </td></tr><tr><td>注射器过滤器13毫米与0.45微米的尼龙膜法（HPLC） </td><td>颇尔生命科学</td><td> 4426 </td></tr><tr><td>麦角甾醇</td><td> Sigma-Aldrich公司</td><td> 45480-50G - F </td></tr><tr><td>闪烁瓶</td><td>厂商VWR </td><td> 66021-602 </td></tr><tr><td>氯化钠</td><td> VICAM </td><td> G1124 </td></tr></tbody></table>

quantification of fungal colonization, sporogenesis, and production of mycotoxins using kernel bioassays

由种子腐烂粮食感染真菌对全球谷物产量最大的经济挑战之一，更不用说对人类和动物健康的严重风险。其中谷物产量中，玉米无疑是受影响最严重的作物，由于粮食的完整性和霉菌毒素的种子污染的病原体引起的损失。玉米种植者和食品和饲料加工两个最普遍的问题霉菌毒素是黄曲霉毒素和伏马毒素， 黄曲霉菌和镰刀菌产生，分别。 分子植物-病原体相互作用的最近的研究已经证明，在了解相关的具体机制与植物的真菌感染和真菌毒素污染1,2,3,4,5,6的承诺。因为许多实验室正在使用的内核分析，研究植物病原体相互作用，有需要为不同的生物参数量化标准的方法，所以来自不同实验室的结果可以交叉进行解释。一个强大的和可重复性的种子的定量分析手段，我们已经制定了在实验室内核检测和随后的方法，以量化霉菌的生长，生物量和霉菌毒素污染。四消毒玉米籽粒真菌悬液（10 6）接种在玻璃小瓶和培养一个预定的期限内。样品瓶，然后选择由血球，麦角甾醇的生物分析，高效液相色谱（HPLC），黄曲霉毒素定量使用AflaTest荧光法，高效液相色谱法和伏马菌素量化为分生孢子枚举。

Watch this Scientific Journal Video about Quantification of Fungal Colonization, Sporogenesis, and Production of Mycotoxins Using Kernel Bioassays at JoVE.com

Quantification of Fungal Colonization, Sporogenesis, and Production of Mycotoxins Using Kernel Bioassays

谷类作物种子感染真菌的破坏，促使大量的研究工作，以便更好地了解植物病原体相互作用。研究种子真菌的相互作用，在实验室环境中，我们开发了一个强大的真菌繁殖，生物质能和使用内核生物测定霉菌毒素污染的量化方法。

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The rotting of grains by seed-infecting fungi poses one of the greatest economic challenges to cereal production worldwide, not to mention serious risks ...

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