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DOI :
10.3791/3731-v
September 14th, 2012
Chapters
0:05
Title
1:45
Dissection of Trachea
2:46
Cleaning and Cutting the Trachea
3:45
Enzyme Digestion and Processing into Single Cell Suspension
6:42
Staining for FACS and Sorting
7:23
Processing Sorted Cells for in vitro Culture and in vivo Model
9:40
Representative Data
11:19
Conclusion
여기 마우스 tracheas의 기초 및 submucosal 글 랜드 덕트 세포의 분리에 대한 프로토콜을 보여줍니다. 우리는 또한을 만들 등쪽 마우스 지방 패드에 줄기 세포를 주입하는 방법을 보여줍니다 생체 내 모델입니다.
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