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Generation of Induced Regulatory T Cells from Primary Human Naïve and Memory T Cells

23.9K Views

14:23 min

April 16th, 2012

DOI :

10.3791/3738-v

April 16th, 2012


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Induced Regulatory T Cells

Chapters in this video

0:05

Title

2:22

Isolation of Human Peripheral Blood Mononuclear Cells (PBMCs) from Buffy Coat

3:58

Magnetic Negative Selection of T Cells from PBMCs using EasySep Enrichment Kit

6:16

Cell Culture Conditions to Induce Regulatory T Cells

7:11

Fluorescence-activated Cell Sorting of Three Populations of T Cells

8:28

Suppression Assay

10:32

Representative Sorting and Suppression Assay Results

13:14

Conclusion

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