Name: high-resolution functional magnetic resonance imaging methods for human midbrain
Uploaded: 2012-05-10T13:00:00
Description: Watch this Scientific Journal Video about High-resolution Functional Magnetic Resonance Imaging Methods for Human Midbrain at JoVE.com

基于这种材料后，根据批准的BCS 1063774由国家科学基金会支持的工作。

1。极角地形刺激和心理物理学为了获得一个极角在SC retinotopic地图的，我们使用一个移动的小圆点为刺激90°楔形（2-9°的可视角度，偏心率平均点速度4°/秒）（ 图1）。据了解，在SC的活动是由申请隐蔽关注5增强，因此，我们在我们的范例中使用注意的任务，以增加可用的信号。上每2秒试验，受试者被指示隐蔽地参加到整个楔形和执行速度的歧视任务，?...

<ol>
	<li>Katyal, S., Zughni, S., Greene, C., Ress, D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Topography+of+covert+visual+attention+in+human+superior+colliculus.">Topography of covert visual attention in human superior colliculus.</a> Journal of Neurophysiology. 104, 3074-3083 (2010).</li><li>Yushkevich, P. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=User-guided+3D+active+contour+segmentation+of+anatomical+structures:+significantly+improved+efficiency+and+reliability.">User-guided 3D active contour segmentation of anatomical structures: significantly improved efficiency and reliability.</a> NeuroImage. 31, 1116-1128 (2006).</li><li>Xu, G., Pan, Q., Bajaj, C. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Discrete+Surface+Modeling+Using+Partial+Differential+Equations.">Discrete Surface Modeling Using Partial Differential Equations.</a> Computer Aided Geometric Design. 23, 125-145 (2006).</li><li>Ress, D., Glover, G. H., Liu, J., Wandell, B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Laminar+profiles+of+functional+activity+in+the+human+brain.">Laminar profiles of functional activity in the human brain.</a> NeuroImage. 34, 74-84 (2007).</li><li>Schneider, K. A., Kastner, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Effects+of+sustained+spatial+attention+in+the+human+lateral+geniculate+nucleus+and+superior+colliculus.">Effects of sustained spatial attention in the human lateral geniculate nucleus and superior colliculus.</a> J. Neurosci. 29, 1784-1795 (2009).</li><li>Glover, G. H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Simple+analytic+spiral+K-space+algorithm.">Simple analytic spiral K-space algorithm.</a> Magn. Reson. Med. 42, 412-415 (1999).</li><li>Glover, G. H., Lai, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Self-navigated+spiral+fMRI:+interleaved+versus+single-shot.">Self-navigated spiral fMRI: interleaved versus single-shot.</a> Magn. Reson. Med. 39, 361-368 (1998).</li><li>Nestares, O., Heeger, D. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Robust+multiresolution+alignment+of+MRI+brain+volumes.">Robust multiresolution alignment of MRI brain volumes.</a> Magn. Reson. Med. 43, 705-715 (2000).</li><li>Engel, S. A., Glover, G. H., Wandell, B. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Retinotopic+organization+in+human+visual+cortex+and+the+spatial+precision+of+functional+MRI.">Retinotopic organization in human visual cortex and the spatial precision of functional MRI.</a> Cereb. Cortex. 7, 181-192 (1997).</li><li>Schneider, K. A., Kastner, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Visual+responses+of+the+human+superior+colliculus:+a+high-resolution+functional+magnetic+resonance+imaging+study.">Visual responses of the human superior colliculus: a high-resolution functional magnetic resonance imaging study.</a> Journal of Neurophysiology. 94, 2491-2503 (2005).</li><li>Cynader, M., Berman, N. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Receptive-field+organization+of+monkey+superior+colliculus.">Receptive-field organization of monkey superior colliculus.</a> Journal of Neurophysiology. 35, 187-201 (1972).</li><li>Robinson, D. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Eye+movements+evoked+by+collicular+stimulation+in+the+alert+monkey.">Eye movements evoked by collicular stimulation in the alert monkey.</a> Vision Research. 12, 1795-1808 (1972).</li><li>Schreiner, C. E., Langner, G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Laminar+fine+structure+of+frequency+organization+in+auditory+midbrain.">Laminar fine structure of frequency organization in auditory midbrain.</a> Nature. 388, 383-385 (1997).</li><li>Baumann, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Orthogonal+representation+of+sound+dimensions+in+the+primate+midbrain.">Orthogonal representation of sound dimensions in the primate midbrain.</a> Nature Neuroscience. 14, 423-425 (2011).</li><li>Malmierca, M. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+discontinuous+tonotopic+organization+in+the+inferior+colliculus+of+the+rat.">A discontinuous tonotopic organization in the inferior colliculus of the rat.</a> J. Neurosci. 28, 4767 (2008).</li><li>Bender, D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Retinotopic+organization+of+macaque+pulvinar.">Retinotopic organization of macaque pulvinar.</a> Journal of Neurophysiology. 46, 672 (1981).</li><li>Grieve, K. L., Acuña, C., Cudeiro, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+primate+pulvinar+nuclei:+vision+and+action.">The primate pulvinar nuclei: vision and action.</a> Trends in Neurosciences. 23, 35-39 (2000).</li><li>Rodriguez-Oroz, M. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+subthalamic+nucleus+in+Parkinson's+disease:+somatotopic+organization+and+physiological+characteristics.">The subthalamic nucleus in Parkinson's disease: somatotopic organization and physiological characteristics.</a> Brain. 124, 1777 (2001).</li><li>Romanelli, P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Microelectrode+recording+revealing+a+somatotopic+body+map+in+the+subthalamic+nucleus+in+humans+with+Parkinson+disease.">Microelectrode recording revealing a somatotopic body map in the subthalamic nucleus in humans with Parkinson disease.</a> Journal of Neurosurgery. 100, 611-618 (2004).</li><li>DeLong, M. R., Crutcher, M. D., Georgopoulos, A. P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Primate+globus+pallidus+and+subthalamic+nucleus:+functional+organization.">Primate globus pallidus and subthalamic nucleus: functional organization.</a> Journal of Neurophysiology. 53, 530 (1985).</li><li>Houeto, J. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Acute+deep-brain+stimulation+of+the+internal+and+external+globus+pallidus+in+primary+Dystonia+functional+mapping+of+the+pallidum.">Acute deep-brain stimulation of the internal and external globus pallidus in primary Dystonia functional mapping of the pallidum.</a> Archives of Neurology. 64, 1281-1286 (2007).</li></ol>

我们的采集和数据分析技术，使测量的高分辨率（1.2毫米像素）人类大脑皮质结构的神经活动。 3杆的螺旋收购减少了生理的噪音，特别是不利于周围的脑功能磁共振成像测量。此外，我们的组织层分割，允许我们进行深入的数据，有助于提高信噪比平均。我们使用这些方法来显示精确的极角地形图的视觉刺激和视觉注意隐蔽在人类SC 1。层分割，也使实验控制下1不同的功能活动?...

high-resolution functional magnetic resonance imaging methods for human midbrain

功能磁共振成像（fMRI）技术是一种非侵入性测量人类大脑活动的相关广泛使用的工具。然而，它的使用大多集中后测量大脑皮质的表面，而不是在皮质下区域，如脑和脑干的活动。皮质功能磁共振成像必须克服两大挑战：空间分辨率和生理噪音。在这里，我们描述的制定执行，在中脑的背侧表面的结构在人类SC高分辨率功能磁共振成像技术的优化方法也可用于图像等脑干和皮层下结构。 高分辨率（1.2毫米像素）的资深大律师的功能磁共振成像需要一个非传统的方法。使用多杆（交错）的螺旋收购1，获得所需的空间采样。由于T 2 * SC的组织是长于皮层，相应的回波时间较长（T E〜40毫秒）用于为最大健康迈兹功能。到涵盖完整程度的SC，8-10片。为每个会话结构解剖也得到相同的功能磁共振成像片处方，这是用来对齐功能的数据到一个高分辨率的参考量。 在一个单独的会话，每个主题中，我们创建一个高分辨率（0.7毫米采样） 使用 T 1加权序列提供了良好的组织对比度的参考量。在参考量，中脑区域分割使用ITK的SNAP软件应用2。这种分割是用来创建一个三维表面的脑，是顺利和准确的3表示。表面顶点和法线用于创建地图从脑表面的深度范围内组织4。 功能数据转化成坐标系统分割的参考量。深入协会的像素使平均的fMRI时间序列数据，在指定的深度范围内，以提高信号质量。数据可视化三维表面呈现。 在我们的实验室中，我们使用这项技术在SC 1的视觉刺激和视觉注意隐蔽和公开的地形图测量。作为一个例子，我们表现出极角的地形表示SC视觉刺激。

Watch this Scientific Journal Video about High-resolution Functional Magnetic Resonance Imaging Methods for Human Midbrain at JoVE.com

High-resolution Functional Magnetic Resonance Imaging Methods for Human Midbrain

本文介绍了使用3T扫描仪在人脑和皮层下结构与1.2毫米采样高分辨率功能磁共振成像技术来执行。使用这些技术来解决视觉刺激在人类上丘（SC）的地形图作为一个例子。

高分辨率人类中脑功能磁共振成像方法

Functional MRI (fMRI) is a widely used tool for non-invasively measuring correlates of human brain activity. However, its use has mostly been focused upon ...

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