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DOI :
10.3791/3749-v
•
9:54 min
December 26th, 2011
Chapters
0:05
Title
1:46
Enzymatic Deglycosylation
3:32
SDS-PAGE of Deglycosylated Samples
6:49
Analysis of hCGβ Enzymatic Deglycosylation
9:02
Conclusion
4:55
Pro-Q Emerald 300 for Detection of Glyclosylated Proteins in SDS-PAGE Gels
使用特定的糖苷酶,以消除通过SDS - PAGE的糖蛋白的糖是一种有价值的的方法来检测蛋白质样品聚糖修改初始糖生物学研究的是一个不错的选择。以下deglycosylation变化可以检测凝胶的流动性的变化或与多糖敏感的试剂染色。
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