Name: measuring left ventricular pressure in late embryonic and neonatal mice
Uploaded: 2012-02-23T13:00:00
Description: Watch this Scientific Journal Video about Measuring Left Ventricular Pressure in Late Embryonic and Neonatal Mice at JoVE.com

资助，这项工作的一部分，由NIH资助HL087653和HL105314。在博士罗伯特Mecham的实验室在华盛顿大学医学院的一些方法。

1。超声和​​压力系统<ol><li>根据制造商的说明（VEVO 770，Visualsonics）的启动超声系统。填写适当的探针（年龄E18 - P7的= 708，年龄&gt; P7的模型707B模型），用蒸馏水和连接的超声系统。探头放置在可调底座的大致方位，要获得一个LV长轴成像平台上安装鼠标，使LV尖向注射胳膊指出。 </li><li>压力系统由一个充满液体的压力传感器，桥式放大器，数据采集系统和数据记录软件（LabChart）。三通旋?...

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	<li>Ji, R. P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Onset+of+cardiac+function+during+early+mouse+embryogenesis+coincides+with+entry+of+primitive+erythroblasts+into+the+embryo+proper.">Onset of cardiac function during early mouse embryogenesis coincides with entry of primitive erythroblasts into the embryo proper.</a> Circ. Res. 92 (2), 133-135 (2003).</li><li>Ishiwata, T., Nakazawa, M., Pu, W. T., Tevosian, S. G., Izumo, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Developmental+changes+in+ventricular+diastolic+function+correlate+with+changes+in+ventricular+myoarchitecture+in+normal+mouse+embryos.">Developmental changes in ventricular diastolic function correlate with changes in ventricular myoarchitecture in normal mouse embryos.</a> Circ. Res. 93 (9), 857-865 (2003).</li><li>Huang, Y., Guo, X., Kassab, G. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Axial+nonuniformity+of+geometric+and+mechanical+properties+of+mouse+aorta+is+increased+during+postnatal+growth.">Axial nonuniformity of geometric and mechanical properties of mouse aorta is increased during postnatal growth.</a> Am. J. Physiol. Heart Circ. Physiol. 290 (2), H657-H664 (2006).</li><li>Ishii, T., Kuwaki, T., Masuda, Y., Fukuda, Y. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Postnatal+development+of+blood+pressure+and+baroreflex+in+mice.">Postnatal development of blood pressure and baroreflex in mice.</a> Auton. Neurosci. 94 (1-2), 34-41 (2001).</li><li>Le, V. P., Knutsen, R. H., Mecham, R. P., Wagenseil, J. E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Decreased+aortic+diameter+and+compliance+precedes+blood+pressure+increases+in+postnatal+development+of+elastin-insufficient+mice.">Decreased aortic diameter and compliance precedes blood pressure increases in postnatal development of elastin-insufficient mice.</a> Am. J. Physiol. Heart Circ. Physiol. , (2011).</li><li>Wagenseil, J. E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Reduced+vessel+elasticity+alters+cardiovascular+structure+and+function+in+newborn+mice.">Reduced vessel elasticity alters cardiovascular structure and function in newborn mice.</a> Circ. Res. 104 (10), 1217-1224 (2009).</li><li>Wagenseil, J. E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+importance+of+elastin+to+aortic+development+in+mice.">The importance of elastin to aortic development in mice.</a> Am. J. Physiol. Heart Circ. Physiol. 299 (2), H257-H264 (2010).</li><li>Ramirez, F., Sakai, L. Y., Rifkin, D. B., Dietz, H. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Extracellular+microfibrils+in+development+and+disease.">Extracellular microfibrils in development and disease.</a> Cell. Mol. Life Sci. 64 (18), 2437-2446 (2007).</li><li>Li, D. Y. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Elastin+point+mutations+cause+an+obstructive+vascular+disease,+supravalvular+aortic+stenosis.">Elastin point mutations cause an obstructive vascular disease, supravalvular aortic stenosis.</a> Hum. Mol. Genet. 6 (7), 1021-1028 (1997).</li><li>Roth, D. M., Swaney, J. S., Dalton, N. D., Gilpin, E. A., Ross, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Impact+of+anesthesia+on+cardiac+function+during+echocardiography+in+mice.">Impact of anesthesia on cardiac function during echocardiography in mice.</a> Am. J. Physiol. Heart Circ. Physiol. 282 (6), H2134-H2140 (2002).</li><li>Phoon, C. K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Imaging+tools+for+the+developmental+biologist:+ultrasound+biomicroscopy+of+mouse+embryonic+development.">Imaging tools for the developmental biologist: ultrasound biomicroscopy of mouse embryonic development.</a> Pediatr. Res. 60 (1), 14-21 (2006).</li></ol>

这里介绍的协议提供了一个LV的压力，在后期的胚胎和新生儿早期小鼠的测量方法。该协议的主要限制是压力系统的时间分辨率。压力信号衰减，因为它从LV旅行通过针传感器，不仅意味着可以记录压力值。最小的阻​​尼可以通过使用尽可能最大的针，但针必须在LV流明，适合不同年龄的小鼠。因为舒张压可近似为零，LV因此动脉收缩压，可以计算平均LV压力测量。虽然额外动脉变量将是理想的（...

<table border="1"><tbody><tr><td> 试剂/设备名称 </td><td> 公司 </td><td> 目录编号 </td><td> 评论 </td></tr><tr><td>高分辨率超声系统</td><td> visualsonics </td><td> VEVO 770 </td><td>或其他适当的超声系统</td></tr><tr><td>高频超声探头</td><td> visualsonics </td><td> 708和707B </td><td></td></tr><tr><td>成像平台和注射手臂</td><td> visualsonics </td><td>成像站2 </td><td>心电图和温度反馈控制平台</td></tr><tr><td>压力传感器</td><td>公元仪器</td><td> MLT 844 </td><td></td></tr><tr><td>桥式放大器</td><td>公元仪器</td><td> ML221 </td><td></td></tr><tr><td>数据采集​​系统</tD&gt; <td>公元仪器</td><td> ML866 </td><td></td></tr><tr><td>数据记录软件</td><td>公元仪器</td><td> LabChart </td><td></td></tr><tr><td>环站和钳</td><td>各供应商</td><td></td><td>在测量过程中要保持压力传感器</td></tr><tr><td>三路活塞，带Luer连接，男性锁</td><td>科尔帕默</td><td> 30600-02 </td><td></td></tr><tr><td> 1/16“编号聚乙烯管材</td><td>科尔帕默</td><td> 06408 </td><td></td></tr><tr><td>男性和女性luers W / 1/16“软管倒刺</td><td>科尔帕默</td><td> 45510-50 45510-00 </td><td></td></tr><tr><td> 24“在每年年底与男性和女性的鲁尔油管</td><td>科尔帕默</td><td> 30600-60 </td><td></td></tr><tr><td> 3日和10毫升的注射器</td><td> BD公司</td><td></td><td></td></tr><tr><td> 30和25G的针头</td><td> BD公司</td><td></td><td> 1.5英寸长</td></tr><tr><td>大本钟压力计</td><td>里斯特</td><td> 1456-100 </td><td></td></tr><tr><td>盐水</td><td>各供应商</td><td></td><td></td></tr><tr><td>肝素</td><td>各供应商</td><td></td><td></td></tr><tr><td>超声凝胶</td><td>帕克</td><td> aquasonic 100 </td><td></td></tr><tr><td>头发卸妆乳液</td><td>奈尔</td><td></td><td></td></tr></tbody></table>

measuring left ventricular pressure in late embryonic and neonatal mice

血压显着增加脊椎动物​​在胚胎和出生后的发展。在小鼠，血流量首先是围绕胚胎一天（五）8.5 1检测。收缩期左心室（LV）压力是E9.5在2毫米汞柱和11毫米汞柱在E14.5 2。在这些中期萌芽阶段，LV是清晰可见，通过创血压测量胸壁肋骨和皮肤，因为没有得到充分的开发。在E14.5和出生（约E21号）的成像方法必须使用查看LV。出生后，平均动脉压力增大30 - 35 3 - 70毫米汞柱从产后天（P）2。超越P20的，可以测量血压与固态导管（即米勒或Scisense）。 P20的之前，这些导管有太大发展小鼠动脉和动脉血压测量必须用自定义拉塑料导管连接到充液压力传感器或玻璃micropipettes ATT痛伺服空压力传感器4。 我们最近的工作表明，血压增幅最大的中后期早在产后5-7小鼠胚胎发生。这种血压的大量增加，可能会影响发展的动脉平滑肌细胞（SMC）的表型和触发重要的机械传导事件。在人类疾病，地方发展动脉的性能是在细胞外基质蛋白（即马凡氏的综合症8和主动脉瓣上狭窄9）的缺陷损害血压，在此期间迅速变化可能有助于疾病的表型和严重程度通过在机械传导的改变信号。因此，重要的是要能够测量血压的变化，人类疾病的小鼠模型中后期胚胎和新生儿时期。 我们描述了在后期测量左室压力的方法胚胎（E18）和产后早期（小 - 20）小鼠。超声引导下，一个充满液体的压力传感器连接到一个针插入的LV。护理是采取维持正常的心脏功能，尤其是在实验协议的小鼠胚胎。有代表性的数据提出并讨论了该协议的限制。

Watch this Scientific Journal Video about Measuring Left Ventricular Pressure in Late Embryonic and Neonatal Mice at JoVE.com

Measuring Left Ventricular Pressure in Late Embryonic and Neonatal Mice

测量左室压力在胚胎和新生小鼠（LV）的描述。压力测量插入到LV连接到一个充满液体的传感器，超声引导下针。必须小心维持正常的心脏功能，在实验协议。

测量中晚期胚胎和新生小鼠左心室压力

Blood pressure increases significantly during embryonic and postnatal development in vertebrate animals. In the mouse, blood flow is first detectable ...

Research

JoVE Journal

Bioengineering

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测量与建模干燥收缩人类角质层

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科研

生物工程

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脊髓损伤后压力溃疡的小鼠模型

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Optical Imaging of Isolated Murine Ventricular Myocytes

分离的月毛心室肌细胞的光学成像

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