Name: measuring left ventricular pressure in late embryonic and neonatal mice
Uploaded: 2012-02-23T13:00:00
Description: Watch this Scientific Journal Video about Measuring Left Ventricular Pressure in Late Embryonic and Neonatal Mice at JoVE.com

इस काम, भाग में एनआईएच HL087653 और HL105314 अनुदान द्वारा वित्त पोषित किया गया है. तरीकों में से कुछ चिकित्सा के वाशिंगटन विश्वविद्यालय के स्कूल में डॉ. रॉबर्ट Mecham की प्रयोगशाला में विकसित किए गए.

1. अल्ट्रासाउंड और दबाव प्रणाली <ol><li> निर्माताओं (Vevo 770, Visualsonics) के निर्देशों के अनुसार अल्ट्रासाउंड प्रणाली शुरू करो. आसुत जल के साथ अल्ट्रासाउंड प्रणाली से कनेक्ट करने के लिए उचित जांच (E18 उम्र P7 = 708 मॉडल, उम्र&...

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	<li>Ji, R. P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Onset+of+cardiac+function+during+early+mouse+embryogenesis+coincides+with+entry+of+primitive+erythroblasts+into+the+embryo+proper.">Onset of cardiac function during early mouse embryogenesis coincides with entry of primitive erythroblasts into the embryo proper.</a> Circ. Res. 92 (2), 133-135 (2003).</li><li>Ishiwata, T., Nakazawa, M., Pu, W. T., Tevosian, S. G., Izumo, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Developmental+changes+in+ventricular+diastolic+function+correlate+with+changes+in+ventricular+myoarchitecture+in+normal+mouse+embryos.">Developmental changes in ventricular diastolic function correlate with changes in ventricular myoarchitecture in normal mouse embryos.</a> Circ. Res. 93 (9), 857-865 (2003).</li><li>Huang, Y., Guo, X., Kassab, G. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Axial+nonuniformity+of+geometric+and+mechanical+properties+of+mouse+aorta+is+increased+during+postnatal+growth.">Axial nonuniformity of geometric and mechanical properties of mouse aorta is increased during postnatal growth.</a> Am. J. Physiol. Heart Circ. Physiol. 290 (2), H657-H664 (2006).</li><li>Ishii, T., Kuwaki, T., Masuda, Y., Fukuda, Y. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Postnatal+development+of+blood+pressure+and+baroreflex+in+mice.">Postnatal development of blood pressure and baroreflex in mice.</a> Auton. Neurosci. 94 (1-2), 34-41 (2001).</li><li>Le, V. P., Knutsen, R. H., Mecham, R. P., Wagenseil, J. E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Decreased+aortic+diameter+and+compliance+precedes+blood+pressure+increases+in+postnatal+development+of+elastin-insufficient+mice.">Decreased aortic diameter and compliance precedes blood pressure increases in postnatal development of elastin-insufficient mice.</a> Am. J. Physiol. Heart Circ. Physiol. , (2011).</li><li>Wagenseil, J. E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Reduced+vessel+elasticity+alters+cardiovascular+structure+and+function+in+newborn+mice.">Reduced vessel elasticity alters cardiovascular structure and function in newborn mice.</a> Circ. Res. 104 (10), 1217-1224 (2009).</li><li>Wagenseil, J. E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+importance+of+elastin+to+aortic+development+in+mice.">The importance of elastin to aortic development in mice.</a> Am. J. Physiol. Heart Circ. Physiol. 299 (2), H257-H264 (2010).</li><li>Ramirez, F., Sakai, L. Y., Rifkin, D. B., Dietz, H. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Extracellular+microfibrils+in+development+and+disease.">Extracellular microfibrils in development and disease.</a> Cell. Mol. Life Sci. 64 (18), 2437-2446 (2007).</li><li>Li, D. Y. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Elastin+point+mutations+cause+an+obstructive+vascular+disease,+supravalvular+aortic+stenosis.">Elastin point mutations cause an obstructive vascular disease, supravalvular aortic stenosis.</a> Hum. Mol. Genet. 6 (7), 1021-1028 (1997).</li><li>Roth, D. M., Swaney, J. S., Dalton, N. D., Gilpin, E. A., Ross, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Impact+of+anesthesia+on+cardiac+function+during+echocardiography+in+mice.">Impact of anesthesia on cardiac function during echocardiography in mice.</a> Am. J. Physiol. Heart Circ. Physiol. 282 (6), H2134-H2140 (2002).</li><li>Phoon, C. K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Imaging+tools+for+the+developmental+biologist:+ultrasound+biomicroscopy+of+mouse+embryonic+development.">Imaging tools for the developmental biologist: ultrasound biomicroscopy of mouse embryonic development.</a> Pediatr. Res. 60 (1), 14-21 (2006).</li></ol>

यहाँ प्रस्तुत प्रोटोकॉल देर से भ्रूण और जल्दी नवजात चूहों में एल.वी. दबाव मापने के लिए एक तरीका प्रदान करता है. इस प्रोटोकॉल के मुख्य सीमा के दबाव प्रणाली के अस्थायी समाधान है. दबाव संकेत के रूप में सुई ?...

<table border="1"><tbody><tr><td> अभिकर्मक / उपकरण का नाम </td><td> कंपनी </td><td> सूचीपत्र संख्या </td><td> टिप्पणियाँ </td></tr><tr><td> उच्च संकल्प अल्ट्रासाउंड प्रणाली </td><td> Visualsonics </td><td> Vevo 770 </td><td> अन्य उपयुक्त अल्ट्रासाउंड प्रणाली या </td></tr><tr><td> उच्च आवृत्ति अल्ट्रासाउंड जांच </td><td> Visualsonics </td><td> 708 और 707B </td><td></td></tr><tr><td> इमेजिंग मंच और इंजेक्शन हाथ </td><td> Visualsonics </td><td> इमेजिंग स्टेशन 2 </td><td> ईसीजी और तापमान मंच की प्रतिक्रिया पर नियंत्रण के साथ </td></tr><tr><td> दबाव transducer </td><td> ई. उपकरण </td><td> 844 एम एल टी </td><td></td></tr><tr><td> पुल प्रवर्धक </td><td> ई. उपकरण </td><td> ML221 </td><td></td></tr><tr><td> डाटा अधिग्रहण प्रणाली </tघ&gt; <td> ई. उपकरण </td><td> ML866 </td><td></td></tr><tr><td> डेटा रिकॉर्डिंग सॉफ्टवेयर </td><td> ई. उपकरण </td><td> LabChart </td><td></td></tr><tr><td> खड़े अंगूठी और दबाना </td><td> विभिन्न आपूर्तिकर्ताओं </td><td></td><td> माप के दौरान दबाव transducer पकड़ </td></tr><tr><td> Luer कनेक्शन के साथ 3 तरह से stopcocks, पुरुष ताला </td><td> कोल परमार </td><td> 30600-02 </td><td></td></tr><tr><td> 1/16 &quot;आईडी Tygon टयूबिंग </td><td> कोल परमार </td><td> 06,408 </td><td></td></tr><tr><td> पुरुष और महिला w / 1/16 &quot;नली आँकड़ी luers के </td><td> कोल परमार </td><td> 45,510-50 45,510-00 </td><td></td></tr><tr><td> प्रत्येक के अंत में 24 पुरुष और महिला Luer साथ टयूबिंग </td><td> कोल परमार </td><td> 30600-60 </td><td></td></tr><tr><td> 3 और 10 एमएल सीरिंज </td><td> बी.डी. बायोसाइंसेज </td><td></td><td></td></tr><tr><td> 30 और 25G सुई </td><td> बी.डी. बायोसाइंसेज </td><td></td><td> लंबाई में 1.5 इंच </td></tr><tr><td> बड़ी बेन दबाव नापने का यंत्र </td><td> Riester </td><td> 1456-100 </td><td></td></tr><tr><td> खारा </td><td> विभिन्न आपूर्तिकर्ताओं </td><td></td><td></td></tr><tr><td> हेपरिन </td><td> विभिन्न आपूर्तिकर्ताओं </td><td></td><td></td></tr><tr><td> अल्ट्रासाउंड जेल </td><td> पार्कर </td><td> 100 aquasonic </td><td></td></tr><tr><td> बाल हटानेवाला लोशन </td><td> नायर </td><td></td><td></td></tr></tbody></table>

measuring left ventricular pressure in late embryonic and neonatal mice

Blood pressure increases significantly during embryonic and postnatal development in vertebrate animals. In the mouse, blood flow is first detectable around embryonic day (E) 8.51. Systolic left ventricular (LV) pressure is 2 mmHg at E9.5 and 11 mmHg at E14.52. At these mid-embryonic stages, the LV is clearly visible through the chest wall for invasive pressure measurements because the ribs and skin are not fully developed. Between E14.5 and birth (approximately E21) imaging methods must be used to view the LV. After birth, mean arterial pressure increases from 30 - 70 mmHg from postnatal day (P) 2 - 353. Beyond P20, arterial pressure can be measured with solid-state catheters (i.e. Millar or Scisense). Before P20, these catheters are too big for developing mouse arteries and arterial pressure must be measured with custom pulled plastic catheters attached to fluid-filled pressure transducers3 or glass micropipettes attached to servo null pressure transducers4.
Our recent work has shown that the greatest increase in blood pressure occurs during the late embryonic to early postnatal period in mice5-7. This large increase in blood pressure may influence smooth muscle cell (SMC) phenotype in developing arteries and trigger important mechanotransduction events. In human disease, where the mechanical properties of developing arteries are compromised by defects in extracellular matrix proteins (i.e. Marfan's Syndrome8 and Supravalvular Aortic Stenosis9) the rapid changes in blood pressure during this period may contribute to disease phenotype and severity through alterations in mechanotransduction signals. Therefore, it is important to be able to measure blood pressure changes during late embryonic and neonatal periods in mouse models of human disease.
We describe a method for measuring LV pressure in late embryonic (E18) and early postnatal (P1 - 20) mice. A needle attached to a fluid-filled pressure transducer is inserted into the LV under ultrasound guidance. Care is taken to maintain normal cardiac function during the experimental protocol, especially for the embryonic mice. Representative data are presented and limitations of the protocol are discussed.

Watch this Scientific Journal Video about Measuring Left Ventricular Pressure in Late Embryonic and Neonatal Mice at JoVE.com

Measuring Left Ventricular Pressure in Late Embryonic and Neonatal Mice

मापने भ्रूण और नवजात चूहों में बाएं निलय (LV) दबाव में वर्णित है. दबाव एक तरल पदार्थ से भरे एक transducer जुड़ा एल.वी. में अल्ट्रासाउंड मार्गदर्शन के तहत सुई डालने से मापा जाता है. देखभाल करने के लिए प्रयोगात्मक प्रोटोकॉल दौरान सामान्य हृदय समारोह को बनाए रखने के लिए लिया जाना चाहिए.

मापने देर भ्रूण और नवजात चूहे में छोड़ दिया ventricular दबाव

Blood pressure increases significantly during embryonic and postnatal development in vertebrate animals. In the mouse, blood flow is first detectable ...

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