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12.0K Views
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11:45 min
March 8th, 2012
DOI :
10.3791/3757-v
Chapters
0:05
Title
2:18
Labeling GP120
3:52
Flow Cell Assembly and Bilayer Preparation: His6-tagged GP120 DH12
6:11
Injection of Cells and Immunofluorescence Staining
7:49
Data Analysis
8:56
Results: Imaging Lck and Fyn at HIV-1 Envelope-induced Virological Synapse
10:53
Conclusion
Summary
本文介绍了一种方法,可视化的全内反射荧光显微镜(TIRF)HIV-1诱导信封上的玻璃支持平面双层病毒学突触的形成。该方法也可以结合免疫荧光染色检测艾滋病毒1信封引起的病毒学突触的形成过程中发生的信号分子的活化和再分配。
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