New York University Langone School of Medicine

2 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Imaging of HIV-1 Envelope-induced Virological Synapse and Signaling on Synthetic Lipid Bilayers

Kathleen C. Prins^*1,2, Gaia Vasiliver-Shamis^*2,3, Michael Cammer^1,2, David Depoil^1,2, Michael L. Dustin², Catarina E. Hioe^1,4

¹Department of Pathology, New York University Langone School of Medicine, ²Program in Molecular Pathogenesis, Marty and Helen Kimmel Center for Biology and Medicine and Skirball Institute for Biomolecular Medicine, ³Laboratory of Molecular Immunogenetics, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, ⁴Veteran Affairs New York Harbor Healthcare System

This article describes a method to visualize formation of an HIV-1 envelope-induced virological synapse on glass supported planar bilayers by total internal reflection fluorescence (TIRF) microscopy. The method can also be combined with immunofluorescence staining to detect activation and redistribution of signaling molecules that occur during HIV-1 envelope-induced virological synapse formation.

Immunology and Infection

Zika Virus Specific Diagnostic Epitope Discovery

Maite Sabalza¹, Cheryl A. Barber¹, William R. Abrams¹, Richard Montagna², Daniel Malamud^1,3

¹Department of Basic Sciences and Craniofacial Development, New York University College of Dentistry, ²Rheonix, Inc., ³Department of Medicine, New York University Langone School of Medicine

In this protocol, we describe a technique to discover Zika virus specific diagnostic peptides using a high-density peptide microarray. This protocol can readly be adapted for other emerging infectious diseases.