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DOI :
10.3791/3766-v
•
12:07 min
March 24th, 2012
Chapters
0:05
Title
2:07
Inoculation of Tomato
3:13
Protein Extraction
4:21
Sample Preparation and Peptide Labeling with cysTMT
6:07
Removal of Non-reacted Tag Sample Fractionation
7:44
Sample Enrichment of cysTMT-labeled Peptides
8:43
Mass Spectrometry Analysis
10:02
CysTMT Results: Profiling Thiol Redox Proteome of Pseudomonas syringae in Tomato
11:11
Conclusion
細胞がストレス状況に遭遇すると活性酸素種のレベルが上昇している。ここでは、3'-3 'ジアミノベンジジン染色などcysTMTラベリングとの酸化還元プロテオームのプロファイリングを行うための質量分析の例を示します Pseudomonas syringaeのトマトの葉を処理した。
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