Name: the tail suspension test
Uploaded: 2012-01-28T21:00:00
Description: Watch this Scientific Journal Video about The Tail Suspension Test at JoVE.com

Denne undersøgelse er blevet støttet af tilskuddet NiHM R01 MH091816 og R21 MH084043 til TDG.

1. Materialer 1. Suspension Box Halen-suspension test (TST) medfører suspension mus over jorden ved deres haler. På det mest grundlæggende niveau, kun den procedure kræver en suspension bar eller hylde afsats, og tape. Dog bør forsøgslederen overveje brugen af ​​en baggrund, der giver optimal kontrast. Desuden er det fornuftigt at tage skridt til at forhindre mus i at observere andre dyr, der bliver testet. I vores laboratorium, bruger vi specielt fremstille...

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Udvikling af TST ved Steru et al. 9 blev påvirket af den tidligere udviklede tvunget svømme test 10-12. Svarende til det tvungne svømme test, i TST mus er placeret i en uundgåelig, men moderat stressende situation. Mangel på flugt adfærd betragtes som immobilitet. Ligesom tvunget svømme test, er TST en test bedste valideret til vurdering af antidepressive virkning af narkotika, men også bruges til at vurdere virkningerne af miljø-, neurobiologiske og genetiske manipulationer ...

<ul><li> Tape </li><li> Timer </li><li> Video Kamera </li><li> White Noise Generator (valgfrit) </li><li> Climbstoppers (frivillig; afhængig af virusstamme, der anvendes) </li></ul>

the tail suspension test

Halen-suspensionsprøvning er en mus adfærdsmæssige test nyttige i screening af potentielle antidepressive lægemidler, og vurdering af andre manipulationer, som forventes at påvirke depression adfærd. Mus er suspenderet fra deres haler med tape, i en sådan position, at det ikke kan undslippe eller holde på nærliggende overflader. Under denne test, seks minutters varighed typisk er den resulterende undslippe orienterede adfærd kvantificeret. Halen-suspensionsprøvning er et værdifuldt redskab inden for lægemiddelforskning for high-throughput screening af potentielle antidepressive stoffer. Her beskriver vi de oplysninger, der kræves for gennemførelsen af ​​denne test med ekstra fokus på potentielle problemer, der måtte opstå, og hvordan man kan undgå dem. Vi tilbyder også en løsning på halen klatring adfærd, et fælles problem, der gør denne test ubrugelig i nogle mus stammer, såsom den meget brugte C57BL / 6. Konkret vi forhindre hale klatring adfærd ved at føre musen halen gennem et lille plasticcylinder før suspensionen. Endelig vil vi beskrive, hvordan du manuelt score den adfærd, der er manifesteret i denne test.

Watch this Scientific Journal Video about The Tail Suspension Test at JoVE.com

The Tail Suspension Test

Halen-suspension test er valideret som en eksperimentel procedure for at vurdere antidepressiv effekt af medicinsk behandling hos mus. Mus er suspenderet af deres haler i seks minutter og flygte-adfærd vurderes. Vi beskriver procedurer, der anvendes på at føre den halen suspension test.

Halen Opslæmningsprøve

The tail-suspension test is a mouse behavioral test useful in the screening of potential antidepressant drugs, and assessing of other manipulations that ...

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