Name: the tail suspension test
Uploaded: 2012-01-28T21:00:00
Description: Watch this Scientific Journal Video about The Tail Suspension Test at JoVE.com

Deze studie werd ondersteund door de subsidie ​​NiMH R01 MH091816 en R21 MH084043 aan TDG.

1. Materialen 1. Vering Box De staart-ophanging test (TST) betreft opschorting van muizen boven de grond door hun staart. Op het meest basale niveau de procedure vereist slechts een schorsing bar of plank richel, en tape. Toch moet de onderzoeker overwegen het gebruik van een achtergrond die een optimaal contrast biedt. Bovendien, is het verstandig om maatregelen te nemen om muizen te voorkomen dat het observeren van andere dieren die worden getest. In ons laboratoriu...

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Ontwikkeling van de TST door Steru et al.. 9 werd beïnvloed door de eerder ontwikkelde geforceerde zwemtest 10-12. Net als bij de gedwongen zwemmen test, in de TST muizen zijn geplaatst in een onontkoombare, maar matig stressvolle situatie. Gebrek aan ontsnappen gedrag wordt beschouwd immobiliteit. Net als de geforceerde zwemtest, de TST is een test best gevalideerd voor de evaluatie van de antidepressieve werking van drugs, maar ook gebruikt om de effecten van milieu-, neurobiologische e...

<ul><li> Band </li><li> Timer </li><li> Videocamera </li><li> White Noise Generator (optioneel) </li><li> Climbstoppers (optioneel, afhankelijk van de stam gebruikt) </li></ul>

the tail suspension test

De staart-vering test is een muis gedragstest nuttig in de screening van potentiële antidepressiva en het beoordelen van andere manipulaties waarvan wordt verwacht dat depressie gerelateerd gedrag beïnvloeden. Muizen worden geschorst door hun staarten met tape, in een dergelijke positie dat het niet kan ontsnappen of vast te houden in de buurt oppervlakken. Tijdens deze test, meestal zes minuten duurt, worden de resulterende ontsnappen georiënteerd gedrag gekwantificeerd. De staart-vering test is een waardevol instrument in drug discovery voor high-throughput screening van kandidaat antidepressivum verbindingen. We beschrijven hier de gegevens die nodig zijn voor de uitvoering van deze test met extra nadruk op de mogelijke problemen die zich kunnen voordoen en hoe ze te vermijden. Wij bieden ook een oplossing voor de staart klimmen gedrag, een gemeenschappelijk probleem dat maakt deze test nutteloos in sommige muizenstammen, zoals de veel gebruikte C57BL / 6. In het bijzonder, voorkomen we staart klimmen gedrag door het passeren van de muis staarten door een klein plasticcilinder vóór de schorsing. Ten slotte hebben we detail hoe u handmatig de score van de gedragingen die zich manifesteren in deze test.

Watch this Scientific Journal Video about The Tail Suspension Test at JoVE.com

The Tail Suspension Test

De staart-vering test is gevalideerd als een experimentele procedure om antidepressieve werking van het geneesmiddel behandelingen in muizen te beoordelen. Muizen worden geschorst door hun staart gedurende zes minuten en ontsnappen gerelateerd gedrag worden beoordeeld. We beschrijven procedures die worden gebruikt bij het uitvoeren van de staart ophanging te testen.

De Staart Suspension Test

The tail-suspension test is a mouse behavioral test useful in the screening of potential antidepressant drugs, and assessing of other manipulations that ...

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