Name: the tail suspension test
Uploaded: 2012-01-28T21:00:00
Description: Watch this Scientific Journal Video about The Tail Suspension Test at JoVE.com

Cette étude a été soutenue par la bourse R01 et R21 Nihm MH091816 MH084043 au TMD.

1. Matériaux 1. Boîte Suspension Le test de suspension arrière (TST) implique la suspension de souris au-dessus du sol par leurs queues. Au niveau le plus élémentaire, la procédure exige seulement une barre de suspension ou un rebord étagère, et du ruban adhésif. Toutefois, l&#39;expérimentateur devrait envisager l&#39;utilisation d&#39;un fond qui fournit un contraste optimal. De plus, il est prudent de prendre des mesures pour empêcher les souris d&#39;ob...

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Développement de la TCT par Steru et al. 9 a été influencée par la force précédemment développé le test de natation 10-12. Similaire au test de la nage forcée, dans le TCT souris sont placées dans une situation inévitable, mais modérément stressante. Manque de comportement de fuite est considéré comme lié immobilité. Comme le test de natation forcée, le TST est un test validé pour la meilleure évaluation de l&#39;efficacité antidépressive de la drogue, mais aussi util...

<ul><li> Bande </li><li> Minuterie </li><li> Caméra Vidéo </li><li> Générateur de bruit blanc (facultatif) </li><li> Climbstoppers (facultatif; selon souche utilisée) </li></ul>

the tail suspension test

Le test de la queue-suspension est un test de la souris comportementales utiles dans le dépistage des potentiels des médicaments antidépresseurs, et d&#39;évaluer d&#39;autres manipulations que l&#39;on s&#39;attend à affecter les comportements de dépression liées. Les souris sont suspendues par la queue avec du ruban adhésif, dans une telle position qu&#39;il ne peut pas s&#39;échapper ou s&#39;accrocher à des surfaces à proximité. Pendant cet essai, généralement de six minutes dans la durée, le comportements de fuite résultant orientés sont quantifiées. Le test de la queue-suspension est un outil précieux dans la découverte de médicaments pour le criblage à haut débit des futurs composés antidépresseurs. Ici, nous décrivons les détails nécessaires pour la mise en œuvre de ce test avec un accent supplémentaire sur les problèmes potentiels qui peuvent survenir et comment les éviter. Nous offrons également une solution à la queue du comportement d&#39;escalade, un problème commun qui rend ce test inutile dans certaines souches de souris, comme le très utilisé C57BL / 6. Plus précisément, nous empêchons la queue des comportements d&#39;escalade en passant la souris queues à travers un petit plastiquecylindre avant la suspension. Enfin, nous détaillons comment manuellement score de comportements qui se manifestent dans ce test.

Watch this Scientific Journal Video about The Tail Suspension Test at JoVE.com

The Tail Suspension Test

Le test de suspension arrière est validé comme une procédure expérimentale pour évaluer l&#39;efficacité antidépressive des traitements médicamenteux chez la souris. Les souris sont suspendues par la queue pendant six minutes et échapper à des comportements liés sont évalués. Nous décrivons les procédures utilisées pour effectuer le test de suspension arrière.

Le test de suspension caudale

The tail-suspension test is a mouse behavioral test useful in the screening of potential antidepressant drugs, and assessing of other manipulations that ...

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