Name: the tail suspension test
Uploaded: 2012-01-28T21:00:00
Description: Watch this Scientific Journal Video about The Tail Suspension Test at JoVE.com

Diese Studie wurde von der Gewährung Nihm R01 und R21 MH091816 MH084043 TDG unterstützt.

1. Materialien 1. Suspension Box Der Schwanz-Suspension-Test (TST) beinhaltet das Suspendieren Mäuse über dem Boden an ihren Schwänzen. Auf der untersten Ebene, das Verfahren erfordert nur eine Suspension bar oder in einem Regal Sims, und Band. Allerdings sollte der Experimentator betrachten den Einsatz von einem Hintergrund, der optimalen Kontrast bietet. Darüber hinaus ist es ratsam, Schritte, um Mäuse aus der Beobachtung anderer Tiere, die getestet werden zu v...

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Die Entwicklung des TST durch Steru et al. 9 wurde durch die bisher entwickelten Forced Swim Test 10-12 beeinflusst. Ähnlich wie bei den Forced Swim Test, in der TST-Mäuse sind in eine unausweichliche, aber mäßig belastenden Situation. Mangelnde entkommen Verhaltensweisen gilt Unbeweglichkeit. Wie die Forced Swim Test ist das TST ein Test am besten für die Bewertung der antidepressiven Wirksamkeit von Medikamenten überprüft, sondern auch verwendet, um die Auswirkungen der Umwelt-, n...

<ul><li> Band </li><li> Timer </li><li> Videokamera </li><li> White Noise Generator (optional) </li><li> Climbstoppers (optional; je nach Stamm) </li></ul>

the tail suspension test

Der Schwanz-Suspension-Test ist eine Maus Verhaltenstest sinnvoll in das Screening von potenziellen Antidepressiva, und die Beurteilung der andere Manipulationen, die voraussichtlich Depression Verhaltensweisen beeinflussen. Mäuse sind durch ihre Schwänze mit Klebeband ausgesetzt, in einer solchen Position, dass es nicht entweichen kann oder halten in der Nähe Oberflächen. Während dieses Tests in der Regel sechs Minuten Dauer werden die daraus resultierenden Flucht orientierte Verhaltensweisen quantifiziert. Der Schwanz-Suspension-Test ist ein wertvolles Werkzeug in der Wirkstoffforschung für das Hochdurchsatz-Screening von potenziellen antidepressiven Verbindungen. Hier beschreiben wir die Einzelheiten der Durchführung dieses Tests mit zusätzlichen Schwerpunkt auf potenzielle Probleme, die auftreten und wie sie vermieden werden müssen. Wir bieten auch eine Lösung für das Heck Klettern Verhalten, ein häufiges Problem, dass dieser Test nutzlos in einigen Mausstämmen macht, wie das weit verbreitete C57BL / 6. Genauer gesagt, verhindern wir tail Klettern Verhalten, indem Mäuseschwänzen durch einen kleinen Kunststoff-Zylinder vor der Aussetzung. Schließlich haben wir ausführlich, wie Sie manuell Gäste die Verhaltensweisen, die in diesem Test manifestiert sind.

Watch this Scientific Journal Video about The Tail Suspension Test at JoVE.com

The Tail Suspension Test

Der Schwanz-Suspension-Test ist als ein experimentelles Verfahren zur antidepressiven Wirksamkeit von medikamentösen Behandlungen bei Mäusen zu bewerten validiert. Mäuse sind an ihren Schwänzen für sechs Minuten unterbrochen und die Flucht Verhaltensweisen bewertet werden. Wir beschreiben die Verfahren bei der Durchführung der Schwanz Suspensionsversuch verwendet.

The Tail Suspension Test-

The tail-suspension test is a mouse behavioral test useful in the screening of potential antidepressant drugs, and assessing of other manipulations that ...

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