Name: the tail suspension test
Uploaded: 2012-01-28T21:00:00
Description: Watch this Scientific Journal Video about The Tail Suspension Test at JoVE.com

इस अध्ययन को अनुदान NIHM MH091816 R01 और R21 TDG MH084043 द्वारा समर्थित किया गया.

1. सामग्री 1. सस्पेंशन बॉक्स पूंछ निलंबन परीक्षण (TST) अपनी दुम द्वारा जमीन के ऊपर निलंबित चूहों शामिल है. सबसे बुनियादी स्तर पर, प्रक्रिया केवल निलंबन पट्टी या शेल्फ कगार, और टेप की आवश्?...

<ol>
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Pharmacol. 47, 379-391 (1978).</li><li>Crowley, J. J., Blendy, J. A., Lucki, I. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Strain-dependent+antidepressant-like+effects+of+citalopram+in+the+mouse+tail+suspension+test.">Strain-dependent antidepressant-like effects of citalopram in the mouse tail suspension test.</a> Psychopharmacology (Berl). 183, 257-264 (2005).</li><li>Karolewicz, B., Paul, I. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Group+housing+of+mice+increases+immobility+and+antidepressant+sensitivity+in+the+forced+swim+and+tail+suspension+tests.">Group housing of mice increases immobility and antidepressant sensitivity in the forced swim and tail suspension tests.</a> European Journal of Pharmacology. 415, 197-201 (2001).</li><li>Lad, H. V., Liu, L., Paya-Cano, J. L., Fernandes, C., Schalkwyk, L. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Quantitative+traits+for+the+tail+suspension+test:+automation,+optimization,+and+BXD+RI+mapping.">Quantitative traits for the tail suspension test: automation, optimization, and BXD RI mapping.</a> Mammalian. Genome. 18, 482-491 (2007).</li><li>Liu, X., Gershenfeld, H. K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Genetic+differences+in+the+tail-suspension+test+and+its+relationship+to+imipramine+response+among+11+inbred+strains+of+mice.">Genetic differences in the tail-suspension test and its relationship to imipramine response among 11 inbred strains of mice.</a> Biol. Psychiatry. 49, 575-581 (2001).</li><li>Ripoll, N., David, D. J., Dailly, E., Hascoet, M., Bourin, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Antidepressant-like+effects+in+various+mice+strains+in+the+tail+suspension+test..">Antidepressant-like effects in various mice strains in the tail suspension test..</a> Behav. Brain. Res.. 143, 193-200 (2003).</li><li>Mineur, Y. S., Belzung, C., Crusio, W. E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Effects+of+unpredictable+chronic+mild+stress+on+anxiety+and+depression-like+behavior+in+mice.">Effects of unpredictable chronic mild stress on anxiety and depression-like behavior in mice.</a> Behav. Brain. Res. 175, 43-50 (2006).</li><li>Thierry, B., Stéru, L., Simon, P., Porsolt, R. D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+tail+suspension+test:+Ethical+considerations.">The tail suspension test: Ethical considerations.</a> Psychopharmacology. 90, 284-285 (1986).</li><li>Cryan, J. F., Mombereau, C., Vassout, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+tail+suspension+test+as+a+model+for+assessing+antidepressant+activity:+review+of+pharmacological+and+genetic+studies+in+mice.">The tail suspension test as a model for assessing antidepressant activity: review of pharmacological and genetic studies in mice.</a> Neurosci. Biobehav. Rev. 29, 571-625 (2005).</li><li>O'Leary, O. F., Cryan, J. F., Gould, T. D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=.">.</a> Mood and Anxiety Related Phenotypes in Mice. 42, 119-137 (2009).</li><li>Detke, M. J., Johnson, J., Lucki, I. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Acute+and+Chronic+Antidepressant+Drug+Treatment+in+the+Rat+Forced+Swimming+Test+Model+of+Depression.">Acute and Chronic Antidepressant Drug Treatment in the Rat Forced Swimming Test Model of Depression.</a> Experimental and Clinical Psychopharmacology. 5, 107-112 (1997).</li><li>Rupniak, N. M. 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A., Hen, R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=.">.</a> Mood and Anxiety related phenotypes in mice: Characterization using behavioral tests. 63, 107-121 (2011).</li><li>Juszczak, G. R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+usage+of+video+analysis+system+for+detection+of+immobility+in+the+tail+suspension+test+in+mice.">The usage of video analysis system for detection of immobility in the tail suspension test in mice.</a> Pharmacology Biochemistry and Behavior. 85, 332-338 (2006).</li><li>Crowley, J. J., Jones, O. '. L. e. a. r. y., F, O., Lucki, I. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Automated+tests+for+measuring+the+effects+of+antidepressants+in+mice.">Automated tests for measuring the effects of antidepressants in mice.</a> Pharmacology Biochemistry and Behavior. 78, 269-274 (2004).</li><li>Gould, T. D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Involvement+of+AMPA+receptors+in+the+antidepressant-like+effects+of+lithium+in+the+mouse+tail+suspension+test+and+forced+swim+test.">Involvement of AMPA receptors in the antidepressant-like effects of lithium in the mouse tail suspension test and forced swim test.</a> Neuropharmacology. 54, 577-587 (2008).</li><li>Gould, T. D., Dao, D. T., Kovacsics, C. E., Gould, T. D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=.">.</a> Mood and Anxiety related phenotypes in mice: Characterization using behavioral tests. 42, (2009).</li></ol>

Steru एट अल द्वारा TST विकास 9 पहले से विकसित मजबूर तैरना 10-12 परीक्षण से प्रभावित किया गया था. मजबूर TST चूहों में तैरना परीक्षण, इसी प्रकार एक अपरिहार्य लेकिन मामूली तनावपूर्ण स्थिति में रखा जाता है....

<ul><li> टेप </li><li> घड़ी </li><li> वीडियो कैमरा </li><li> सफेद शोर जनरेटर (वैकल्पिक) </li><li> Climbstoppers (वैकल्पिक आधार पर तनाव इस्तेमाल किया) </li></ul>

the tail suspension test

परीक्षण पूंछ - निलंबन एक माउस व्यवहार संभावित एंटी दवाओं की जांच में उपयोगी परीक्षण है, और है कि अवसाद संबंधी व्यवहार को प्रभावित करने की उम्मीद कर रहे हैं अन्य जोड़तोड़ के आकलन. चूहे अपनी दुम द्वारा टेप के साथ निलंबित कर रहे हैं एक ऐसी स्थिति है कि यह बच या नहीं पास के सतहों पर पकड़ कर सकते हैं,. इस परीक्षण के दौरान, आम तौर पर अवधि में छह मिनट, जिसके परिणामस्वरूप बच उन्मुख व्यवहार मात्रा निर्धारित कर रहे हैं. पूंछ निलंबन परीक्षण भावी एंटी यौगिकों के उच्च throughput स्क्रीनिंग के लिए दवाओं की खोज में एक महत्वपूर्ण उपकरण है. यहाँ, हम कि होते हैं और उन्हें कैसे से बचने के लिए हो सकता है संभावित समस्याओं पर अतिरिक्त जोर देने के साथ इस परीक्षण के कार्यान्वयन के लिए आवश्यक जानकारी का वर्णन करता है. हम भी पूंछ चढ़ाई व्यवहार करने के लिए एक समाधान है, व्यापक रूप से इस्तेमाल किया C57BL / 6 के रूप में एक आम समस्या यह है कि इस परीक्षण के कुछ माउस उपभेदों में बेकार renders, प्रदान करते हैं. विशेष रूप से, हम एक छोटे से प्लास्टिक के माध्यम से माउस पूंछ से गुजर रहा पूंछ चढ़ाई के व्यवहार को रोकने केनिलंबन से पहले सिलेंडर. अंत में, हम विस्तार से मैन्युअल रूप से व्यवहार है कि इस परीक्षा में प्रकट होते हैं कैसे स्कोर करने के लिए.

Watch this Scientific Journal Video about The Tail Suspension Test at JoVE.com

The Tail Suspension Test

पूंछ निलंबन परीक्षण करने के लिए एक प्रयोगात्मक चूहों में दवा उपचार के एंटी प्रभावकारिता का मूल्यांकन करने की प्रक्रिया के रूप में मान्य है. चूहे अपनी दुम द्वारा छह मिनट के लिए निलंबित कर दिया है और भागने से संबंधित व्यवहार का मूल्यांकन कर रहे हैं. हम पूंछ निलंबन परीक्षण में इस्तेमाल किया प्रक्रियाओं का वर्णन.

टेल सस्पेंशन टेस्ट

The tail-suspension test is a mouse behavioral test useful in the screening of potential antidepressant drugs, and assessing of other manipulations that ...

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Neuroscience

One Dimensional Turing-Like Handshake Test for Motor Intelligence

एक आयामी ट्यूरिंग तरह मोटर खुफिया के लिए हाथ मिलाना टेस्ट

In the Turing test, a computer model is deemed to "think intelligently" if it can generate answers that are not distinguishable from those of a human.  ...

Morris Water Maze Test for Learning and Memory Deficits in Alzheimer&#39;s Disease Model Mice

Morris Water Maze Test for Learning and Memory Deficits in Alzheimer's Disease Model Mice

मॉरिस जल सीखने और स्मृति घाटे के लिए भूलभुलैया टेस्ट अल्जाइमर रोग मॉडल चूहे में

The Morris Water Maze (MWM) was first established by neuroscientist Richard G. Morris in 1981 in order to test hippocampal-dependent learning, including ...

The Mouse Forced Swim Test

माउस जबरिया तैरो टेस्ट

The forced swim test is a rodent behavioral test used for evaluation of antidepressant drugs, antidepressant efficacy of new compounds, and experimental ...

Closed-loop Neuro-robotic Experiments to Test Computational Properties of Neuronal Networks

बंद लूप न्यूरो रोबोट प्रयोगों neuronal नेटवर्क के कम्प्यूटेशनल गुणों का परीक्षण करने के लिए

Information coding in the Central Nervous System (CNS) remains unexplored. There is mounting evidence that, even at a very low level, the representation ...

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अनुजंघास्थिक तंत्रिका चोट विधि: एक विश्वसनीय परख की पहचान करने और टेस्ट कि मरम्मत neuromuscular जंक्शनों कारक

The neuromuscular junction (NMJ) undergoes deleterious structural and functional changes as a result of aging, injury and disease. Thus, it is imperative ...

A Computerized Test Battery to Study Pharmacodynamic Effects on the Central Nervous System of Cholinergic Drugs in Early Phase Drug Development

प्रारंभिक चरण दवा विकास में कोलीनर्जिक दवाओं के केंद्रीय तंत्रिका तंत्र पर Pharmacodynamic प्रभाव का अध्ययन करने के लिए एक कंप्यूटरीकृत परीक्षण बैटरी

Investigating potential pharmacodynamic effects in an early phase of central nervous system (CNS) drug research can provide valuable information for ...

Diffuse Reflectance Spectroscopy: Getting the Capillary Refill Test Under One's Thumb

फैलाना चिंतनशील स्पेक्ट्रोस्कोपी: एक अंगूठे के तहत केशिका रिफिल परीक्षण हो रही है

The capillary refill test was introduced in 1947 to help estimate circulatory status in critically ill patients. Guidelines commonly state that refill ...

An Objective and Reproducible Test of Olfactory Learning and Discrimination in Mice

घ्राण सीखने और चूहों में भेदभाव का एक उद्देश्य और Reproducible परीक्षण

Olfaction is the predominant sensory modality in mice and influences many important behaviors, including foraging, predator detection, mating, and ...

Frequent Tail-tip Blood Sampling in Mice for the Assessment of Pulsatile Luteinizing Hormone Secretion

गुणवाला Luteinizing हार्मोन स्राव के आकलन के लिए चूहों में लगातार पूंछ-टिप रक्त का नमूना

In many endocrine systems, circulating factors or hormones are not released continuously, but are secreted as a discrete pulse in response to a releasing ...

A Metric Test for Assessing Spatial Working Memory in Adult Rats Following Traumatic Brain Injury

दर्दनाक मस्तिष्क की चोट के बाद वयस्क चूहों में स्थानिक कार्य स्मृति का आकलन करने के लिए एक मीट्रिक परीक्षण

Impairments to sensory, short-term, and long-term memory are common side effects after traumatic brain injury (TBI). Due to the ethical limitations of ...