Name: the tail suspension test
Uploaded: 2012-01-28T21:00:00
Description: Watch this Scientific Journal Video about The Tail Suspension Test at JoVE.com

Questo studio è stato supportato dal NIHM concedere MH091816 R01 e R21 MH084043 di TDG.

1. Materiale 1. Sospensione Box La coda-sospensione di prova (TST) comporta topi sospensione dal suolo per la coda. Al livello più elementare, la procedura richiede solo una barra di sospensione o sporgenza scaffale, e nastro. Tuttavia, lo sperimentatore dovrebbe considerare l&#39;uso di un fondo che offre un contrasto ottimale. Inoltre, è prudente prendere misure per evitare che i topi da osservare altri animali che sono in fase di test. Nel nostro laboratorio, uti...

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Sviluppo del TST da Steru et al. 9 è stato influenzato dal sviluppato in precedenza costretto nuotare prova 10-12. Simile al test di nuoto forzato, nel TST topi si trovano in una situazione inevitabile, ma moderatamente stressante. La mancanza di comportamento di fuga relativo è considerato immobilità. Come per il test di nuoto forzato, il TST è un test migliore validato per la valutazione dell&#39;efficacia dei farmaci antidepressivi, ma anche utilizzato per valutare gli effetti ambie...

<ul><li> Nastro </li><li> Timer </li><li> Videocamera </li><li> Generatore di rumore bianco (opzionale) </li><li> Climbstoppers (opzionale, a seconda ceppo utilizzato) </li></ul>

the tail suspension test

La coda-sospensione di prova è un test di topo comportamentale utile per lo screening di potenziali farmaci antidepressivi, e la valutazione di altre manipolazioni che si prevede di influenzare i comportamenti depressione correlati. I topi sono sospesi per la coda con del nastro adesivo, in una posizione tale che non può sfuggire o resistere alle superfici vicine. Durante questa prova, in genere di sei minuti di durata, i comportamenti di fuga risulta orientata sono quantificati. La coda-sospensione di prova è uno strumento prezioso nella scoperta di nuovi farmaci per high-throughput screening di potenziali composti antidepressivi. Qui, descriviamo i dati necessari per l&#39;attuazione di questo test, con ulteriore enfasi sugli eventuali problemi che possono verificarsi e come evitarli. Offriamo inoltre una soluzione al comportamento coda arrampicata, un problema comune che rende questo test inutile, in alcuni ceppi di topo, come il diffuso C57BL / 6. In particolare, evitare la coda dei comportamenti salire passando la coda del mouse di plastica con una piccolacilindro prima della sospensione. Infine, dettaglio come per segnare manualmente i comportamenti che si manifestano in questo test.

Watch this Scientific Journal Video about The Tail Suspension Test at JoVE.com

The Tail Suspension Test

La coda-sospensione in esame è convalidato come una procedura sperimentale per valutare l&#39;efficacia di trattamenti farmacologici antidepressivi nei topi. I topi sono sospesi per la coda per sei minuti e la fuga comportamenti correlati sono valutati. Si descrivono le modalità utilizzate nella conduzione della prova sospensione coda.

La coda di test Sospensione

The tail-suspension test is a mouse behavioral test useful in the screening of potential antidepressant drugs, and assessing of other manipulations that ...

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