Name: the tail suspension test
Uploaded: 2012-01-28T21:00:00
Description: Watch this Scientific Journal Video about The Tail Suspension Test at JoVE.com

本研究では、助成金NIHM R01 MH091816とTDGのR21 MH084043によってサポートされています。

1。材料 1。サスペンションボックス尾懸垂試験（TST）は、彼らの尾で地面からマウスを中断伴います。最も基本的なレベルでは、手順はサスペンションのバーや棚の棚、およびテープが必要です。しかし、実験者は、最適なコントラストを提供するバックグラウンドの使用を考慮する必要があります。さらに、それはテストされている他の動物を観察す...

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A., Hen, R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=.">.</a> Mood and Anxiety related phenotypes in mice: Characterization using behavioral tests. 63, 107-121 (2011).</li><li>Juszczak, G. R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+usage+of+video+analysis+system+for+detection+of+immobility+in+the+tail+suspension+test+in+mice.">The usage of video analysis system for detection of immobility in the tail suspension test in mice.</a> Pharmacology Biochemistry and Behavior. 85, 332-338 (2006).</li><li>Crowley, J. J., Jones, O. '. 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Steru らによるTSTの開発9は、以前開発した強制水泳試験10月12日影響を受けていた。 TSTマウスの強制水泳試験、似ては避けられないが適度にストレスの多い状況に置かれている。エスケープ関連行動の欠如が不動とみなされます。強制水泳試験と同様に、TSTはテスト最良の薬の抗うつ効果を評価するための検証だけでなく、環境への神経生物学的、および遺伝的?...

<ul><li>テープ</li><li>タイマー</li><li>ビデオカメラ</li><li>ホワイトノイズ発生器（オプション） </li><li> Climbstoppers（オプション、使用される菌株に応じて） </li></ul>

the tail suspension test

尾懸垂試験は、潜在的な抗うつ薬のスクリーニングに有用なマウスの行動テストで、うつ病に関連する行動に影響を与えないことが予想されている他の操作の査定。マウスは、それがエスケープまたは近くの表面に保持できないような位置に、テープで彼らの尾によって停止されています。このテスト中は、通常は6分間持続時間で、結果として得られるエスケープ指向の振る舞い​​が定量化されています。尾懸垂試験は、将来の抗うつ薬化合物のハイスループットスクリーニングのための創薬に有用なツールです。ここでは、発生する可能性があると、それらを回避する方法を潜在的な問題についての追加重点を置いて、このテストの実施に必要な詳細を説明します。我々はまた、広く使用されているC57BL / 6とテールクライミング動作を解決する、いくつかのマウス系統では、このテストは役に立たないレンダリングする共通の問題を、提供しています。具体的には、小さなプラスチックを通してマウスの尾を渡すことによって、テール登山行動を防ぐ前のサスペンションのシリンダー。最後に、どのように手動でこのテストで明らかにされている行動を獲得するために我々詳細。

Watch this Scientific Journal Video about The Tail Suspension Test at JoVE.com

The Tail Suspension Test

尾懸垂試験は、マウスにおける薬物治療の抗うつ効果を評価するための実験手順として検証されます。マウスは、6分間その尾によって中断され、エスケープ関連の行動が評価されています。我々は、尾懸垂試験の実施に使用される手順を説明します。

尾懸垂テスト

The tail-suspension test is a mouse behavioral test useful in the screening of potential antidepressant drugs, and assessing of other manipulations that ...

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Neuroscience

One Dimensional Turing-Like Handshake Test for Motor Intelligence

モーター知能一次元チューリングライクハンドシェイクテスト

In the Turing test, a computer model is deemed to "think intelligently" if it can generate answers that are not distinguishable from those of a human.  ...

Morris Water Maze Test for Learning and Memory Deficits in Alzheimer&#39;s Disease Model Mice

Morris Water Maze Test for Learning and Memory Deficits in Alzheimer's Disease Model Mice

アルツハイマー病モデルマウスにおける学習記憶障害のためのモリス水迷路試験

The Morris Water Maze (MWM) was first established by neuroscientist Richard G. Morris in 1981 in order to test hippocampal-dependent learning, including ...

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マウス強制水泳試験

The forced swim test is a rodent behavioral test used for evaluation of antidepressant drugs, antidepressant efficacy of new compounds, and experimental ...

Closed-loop Neuro-robotic Experiments to Test Computational Properties of Neuronal Networks

閉ループ神経のロボット実験は、神経回路網の計算特性を試験すること

Information coding in the Central Nervous System (CNS) remains unexplored. There is mounting evidence that, even at a very low level, the representation ...

The Fibular Nerve Injury Method: A Reliable Assay to Identify and Test Factors That Repair Neuromuscular Junctions

腓骨神経損傷方法：神経筋接合部を修復信頼性の高い同定するためのアッセイおよびテスト要因

The neuromuscular junction (NMJ) undergoes deleterious structural and functional changes as a result of aging, injury and disease. Thus, it is imperative ...

A Computerized Test Battery to Study Pharmacodynamic Effects on the Central Nervous System of Cholinergic Drugs in Early Phase Drug Development

創薬初期段階におけるコリン作動性薬物の中枢神経系の薬理効果を研究するコンピュータ テスト バッテリー

Investigating potential pharmacodynamic effects in an early phase of central nervous system (CNS) drug research can provide valuable information for ...

Diffuse Reflectance Spectroscopy: Getting the Capillary Refill Test Under One's Thumb

拡散反射率分光: 取得 1 つの親指の下の毛細血管リフィル テスト

The capillary refill test was introduced in 1947 to help estimate circulatory status in critically ill patients. Guidelines commonly state that refill ...

An Objective and Reproducible Test of Olfactory Learning and Discrimination in Mice

嗅覚学習とマウスにおける差別の客観的かつ再現性のあるテスト

Olfaction is the predominant sensory modality in mice and influences many important behaviors, including foraging, predator detection, mating, and ...

Frequent Tail-tip Blood Sampling in Mice for the Assessment of Pulsatile Luteinizing Hormone Secretion

拍動性の黄体形成ホルモンの分泌の評価のためマウス尾先端採血を頻繁に

In many endocrine systems, circulating factors or hormones are not released continuously, but are secreted as a discrete pulse in response to a releasing ...

A Metric Test for Assessing Spatial Working Memory in Adult Rats Following Traumatic Brain Injury

外傷性脳損傷後の成人ラットの空間ワーキングメモリ評価のための測定基準試験

Impairments to sensory, short-term, and long-term memory are common side effects after traumatic brain injury (TBI). Due to the ethical limitations of ...