Name: the tail suspension test
Uploaded: 2012-01-28T21:00:00
Description: Watch this Scientific Journal Video about The Tail Suspension Test at JoVE.com

Denne studien har vært støttet av tilskuddet NihM R01 MH091816 og R21 MH084043 til TDG.

1. Materialer 1. Suspensjon Box Halen-oppheng test (TST) innebærer å utsette mus over bakken av halene. På det mest grunnleggende nivået, krever prosedyren bare en suspensjon bar eller hylle avsatsen, og tape. Imidlertid bør eksperimentator vurdere bruken av en bakgrunn som gir optimal kontrast. I tillegg er det klokt å ta skritt for å hindre mus fra å observere andre dyr som blir testet. I vårt laboratorium, bruker vi spesielt produsert halen suspensjon boks...

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Utvikling av TST ved Steru et al. 9 ble påvirket av tidligere utviklet tvunget svømme test 10-12. I likhet med tvungen svømme test, i TST mus er plassert i en uunngåelig, men moderat stressende situasjon. Mangel på flukt atferd anses immobilitet. I likhet med tvang svømme test, er TST en test beste validert for evaluering av antidepressive effekten av narkotika, men også brukt til å evaluere effekten av miljømessige, nevrobiologisk og genetisk manipulering 13-18. I mots...

<ul><li> Tape </li><li> Timer </li><li> Videokamera </li><li> White Noise Generator (valgfritt) </li><li> Climbstoppers (valgfritt, avhengig stamme) </li></ul>

the tail suspension test

Halen-oppheng test er en mus atferdsdata test nyttig i screening av potensielle antidepressiva, og vurdering av andre manipulasjoner som forventes å påvirke depresjon relatert atferd. Mus er suspendert av halene med tape i en slik posisjon at den ikke kan rømme eller holde på nærliggende overflater. Under denne testen, vanligvis seks minutter i varighet, er den resulterende unnslippe orientert atferd tallfestet. Halen-oppheng test er et verdifullt verktøy i medisiner for high-throughput screening av potensielle antidepressiva forbindelser. Her beskriver vi detaljene som kreves for gjennomføring av denne testen med ekstra vekt på potensielle problemer som kan oppstå og hvordan unngå dem. Vi tilbyr også en løsning til halen klatring oppførsel, et vanlig problem som gjør denne testen ubrukelig i noen mus stammer, som brukte C57BL / 6. Spesifikt, hindrer vi hale klatring atferd ved å passere musa haler gjennom et lite plastsylinder før suspensjon. Til slutt, vi detalj hvordan du manuelt score atferd som er manifestert i denne testen.

Watch this Scientific Journal Video about The Tail Suspension Test at JoVE.com

The Tail Suspension Test

Halen-oppheng testen er validert som en eksperimentell prosedyre å vurdere antidepressiv effekt av medikamentell behandling hos mus. Mus er suspendert av halene i seks minutter og rømme-relaterte atferd vurderes. Vi beskriver prosedyrer som brukes i å gjennomføre halen suspensjon test.

The tail-suspension test is a mouse behavioral test useful in the screening of potential antidepressant drugs, and assessing of other manipulations that ...

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