Name: the tail suspension test
Uploaded: 2012-01-28T21:00:00
Description: Watch this Scientific Journal Video about The Tail Suspension Test at JoVE.com

Este estudo foi apoiado pelo NIHM conceder R01 e R21 MH091816 MH084043 para TDG.

1. Materiais 1. Box suspensão O teste de tail-suspensão (TST) envolve ratos suspensão acima do solo por suas caudas. No nível mais básico, o procedimento requer apenas uma barra de suspensão ou borda de prateleira, e fita. No entanto, o pesquisador deve considerar o uso de um fundo que proporciona um contraste perfeito. Além disso, é prudente tomar medidas para evitar ratos a partir da observação de outros animais que estão sendo testados. Em nosso laborat?...

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Desenvolvimento do TST por Steru et al. 9 foi influenciado pela desenvolvidos anteriormente teste de natação forçada 10-12. Semelhante ao teste de natação forçada, no TST ratos são colocados em uma situação inevitável, mas moderadamente estressantes. Falta de comportamento de fuga é considerada relacionadas imobilidade. Como o teste de natação forçada, o TST é um teste validado para a melhor avaliação de eficácia antidepressiva de drogas, mas também usado para avaliar os...

<ul><li> Fita </li><li> Cronômetro </li><li> Filmadora </li><li> Gerador de ruído branco (opcional) </li><li> Climbstoppers (opcional; dependendo cepa utilizada) </li></ul>

the tail suspension test

O teste de tail-suspensão é um teste comportamental do rato úteis na triagem de potenciais medicamentos antidepressivos e avaliação de outras manipulações que são esperados para influenciar os comportamentos relacionados com a depressão. Os ratos são suspensos por suas caudas com fita adesiva, em uma posição tal que não possa fugir ou agarrar a superfícies próximas. Durante este teste, normalmente seis minutos de duração, os comportamentos de fuga resultando orientados são quantificados. O teste de tail-suspensão é uma valiosa ferramenta na descoberta de medicamentos de alto throughput screening de potenciais compostos do antidepressivo. Aqui, descrevemos os detalhes necessários para a implementação deste teste, com ênfase adicional sobre potenciais problemas que podem ocorrer e como evitá-los. Nós também oferecemos uma solução para o comportamento da cauda de escalada, um problema comum que torna este teste inútil em algumas linhagens de camundongos, como o C57BL amplamente utilizado / 6. Especificamente, evitar comportamentos de cauda subindo, passando rabos de rato através de um plástico pequenocilindro antes da suspensão. Finalmente, detalhes como pontuação manualmente os comportamentos que se manifestam neste teste.

Watch this Scientific Journal Video about The Tail Suspension Test at JoVE.com

The Tail Suspension Test

O teste de tail-suspensão é validado como um procedimento experimental para avaliar a eficácia antidepressiva de tratamentos com drogas em camundongos. Os ratos são suspensos por suas caudas de seis minutos e escapar comportamentos relacionados são avaliados. Descrevemos os procedimentos utilizados na condução do teste de suspensão da cauda.

O Teste de Suspensão da cauda

The tail-suspension test is a mouse behavioral test useful in the screening of potential antidepressant drugs, and assessing of other manipulations that ...

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