Name: the tail suspension test
Uploaded: 2012-01-28T21:00:00
Description: Watch this Scientific Journal Video about The Tail Suspension Test at JoVE.com

Este estudio ha sido financiado por la beca R01 y R21 NiHM MH091816 MH084043 a TDG.

1. Materiales 1. Suspensión Caja La prueba de la cola de suspensión (TST) implica la suspensión de los ratones por encima del suelo por la cola. En el nivel más básico, el procedimiento sólo requiere de una barra de suspensión o repisa estante, y la cinta. Sin embargo, el investigador debe considerar el uso de un fondo que ofrece un contraste óptimo. Además, es prudente tomar medidas para evitar que los ratones de la observación de otros animales que están ...

<ol>
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Desarrollo de la TST por Steru et al. 9 fue influenciado por el desarrollado anteriormente obligado nadar la prueba 10-12. Similar a la prueba de natación forzada, en el TST ratones se colocan en una situación ineludible, pero moderadamente estresantes. La falta de conducta de evitación se considera relacionada con la inmovilidad. Al igual que la prueba de natación forzada, la TST es una prueba mejor validados para la evaluación de la eficacia antidepresiva de los medicamentos, pero t...

<ul><li> Cinta </li><li> Temporizador </li><li> Cámara de video </li><li> Generador de ruido blanco (opcional) </li><li> Climbstoppers (opcional, dependiendo de la cepa utilizada) </li></ul>

the tail suspension test

La prueba de la cola de suspensión es una prueba de comportamiento del ratón útil en la detección de potenciales fármacos antidepresivos, y la evaluación de otras manipulaciones que se espera que afecte a los comportamientos relacionados con la depresión. Los ratones son suspendidos por la cola con la cinta, en una posición tal que no puede escapar o aferrarse a las superficies cercanas. Durante esta prueba, normalmente de seis minutos de duración, las conductas de escape resultante orientado se cuantifican. La prueba de la cola de suspensión es una herramienta valiosa en el descubrimiento de fármacos para el cribado de alto rendimiento de los futuros antidepresivos. A continuación, describimos los detalles necesarios para la ejecución de esta prueba con especial hincapié en los problemas potenciales que pueden ocurrir y cómo evitarlos. También ofrecemos una solución para el comportamiento de la cola de escalada, un problema común que hace que esta prueba inútil en algunas cepas de ratón, como el ampliamente utilizado C57BL / 6. En concreto, podemos prevenir conductas cola subiendo al pasar colas de ratón a través de un pequeño de plásticocilindro antes de la suspensión. Por último, se detalla la forma de puntuación de forma manual los comportamientos que se manifiestan en esta prueba.

Watch this Scientific Journal Video about The Tail Suspension Test at JoVE.com

The Tail Suspension Test

La prueba de la cola de suspensión se valida como un procedimiento experimental para evaluar la eficacia de los tratamientos farmacológicos antidepresivos en ratones. Los ratones son suspendidos por la cola durante seis minutos y escapar de conductas relacionadas con la evaluación son. Se describen los procedimientos utilizados en la realización de la prueba de suspensión de la cola.

La prueba de la suspensión trasera

The tail-suspension test is a mouse behavioral test useful in the screening of potential antidepressant drugs, and assessing of other manipulations that ...

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