Name: the tail suspension test
Uploaded: 2012-01-28T21:00:00
Description: Watch this Scientific Journal Video about The Tail Suspension Test at JoVE.com

Denna studie har fått stöd av bidraget NIHM R01 MH091816 och R21 MH084043 till TDG.

1. Material 1. Fjädring Box Svansen-suspensionsprov (TST) innebär upphävande möss ovanför marken av deras svansar. På den mest grundläggande nivån, krävs att det förfarande bara en suspension bar eller hylla avsats, och tejp. Dock bör försöksledaren överväga att använda en bakgrund som ger optimal kontrast. Dessutom är det klokt att vidta åtgärder för att hindra möss från att observera andra djur som testas. I vårt laboratorium använder vi spec...

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Utveckling av TST genom Steru et al. Var 9 påverkades av tvingade tidigare utvecklat simma testa 10-12. I likhet med tvingades simma testet i TST möss är placerade i en ofrånkomlig men måttligt stressande situation. Brist på flykt beteenden anses orörlighet. Liksom de tvingades simma testet är TST ett test bäst validerade för utvärdering av den antidepressiva effekten av läkemedel, men också för att utvärdera effekterna av miljö-, neurobiologiska och genetiska manipulatione...

<ul><li> Tape </li><li> Timer </li><li> Videokamera </li><li> White Noise Generator (tillval) </li><li> Climbstoppers (frivilligt, beroende på stam som använts) </li></ul>

the tail suspension test

Svansen-fjädring test är en mus beteende testa användbara vid screening av potentiella antidepressiva läkemedel, och bedömning av andra manipulationer som förväntas påverka depression beteenden. Möss är upphängd i sina svansar med tejp, i ett sådant läge att den inte kan fly eller hålla på till närliggande ytor. Under detta test, vanligtvis sex minuter i längd, är den resulterande fly orienterade beteenden kvantifieras. Svansen-fjädring test är ett värdefullt verktyg för läkemedelsutveckling för high-throughput screening av blivande antidepressiva föreningar. Här beskriver vi de uppgifter som krävs för att genomföra detta test med ytterligare tonvikt på potentiella problem som kan uppstå och hur man undviker dem. Vi erbjuder också en lösning på svansen klättring beteende, ett vanligt problem som gör detta test värdelös i vissa mus stammar, som används allmänt C57BL / 6. Specifikt förhindrar vi svans klättring beteenden genom att passera musen svansar genom ett litet plastcylindern innan avstängning. Slutligen har vi i detalj hur du manuellt poäng de beteenden som manifesteras i detta test.

Watch this Scientific Journal Video about The Tail Suspension Test at JoVE.com

The Tail Suspension Test

Svansen-fjädring test valideras som en experimentell procedur för att bedöma den antidepressiva effekten av läkemedelsbehandling hos möss. Möss är upphängd i sina svansar i sex minuter och fly-relaterade beteenden bedöms. Vi beskriver metoder som används i utför testet svansen suspension.

Svansen suspensionsprov

The tail-suspension test is a mouse behavioral test useful in the screening of potential antidepressant drugs, and assessing of other manipulations that ...

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Neuroscience

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