Name: the tail suspension test
Uploaded: 2012-01-28T21:00:00
Description: Watch this Scientific Journal Video about The Tail Suspension Test at JoVE.com

Bu çalışmada, hibe NIHM, R01 MH091816 ve R21 MH084043 TDG için tarafından desteklenen olmuştur.

1. Malzemeler 1. Süspansiyon Kutusu Kuyruk süspansiyon testi (TST), fareler onların kuyrukları tarafından yerden askıya içerir. En temel düzeyde, yordam, yalnızca bir süspansiyon çubuğu veya raf çıkıntı ve bant gerektirir. Ancak, deneyci optimum kontrast sağlayan bir arka plan göz önünde bulundurmalıdır. Buna ek olarak, test edilmekte olan diğer hayvanlar gözlemleyerek fareler önlemek için adımlar atması için ihtiyatlı olduğunu. Laboratu...

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Steru ark TST Kalkınma 9 daha önce geliştirilen zorla yüzmeye testi 10-12 etkilenmiştir. TST farelerde zorla yüzmeye testi, kaçınılmaz ama orta derecede stresli bir durumda yerleştirilir. Eksikliği, hareketsizlik kaçış ilgili davranış olarak kabul edilir. Zorunlu yüzme testi gibi, TST, antidepresan ilaçların etkinliğinin değerlendirilmesi için en iyi valide test, ancak, aynı zamanda çevresel, nörobiyolojik ve genetik manipülasyonlar 13-18 etkilerini de?...

<ul><li> Bant </li><li> Timer </li><li> Video Kamera </li><li> White Noise Jeneratör (isteğe bağlı) </li><li> Climbstoppers (isteğe bağlı; suşu kullanılmaktadır üzerine bağlı) </li></ul>

the tail suspension test

Tail-süspansiyon test tarama potansiyel antidepresan ilaçlar yararlı bir fare davranış testi ve depresyon ile ilgili davranışlarını etkilemesi beklenmektedir diğer manipülasyonlar değerlendirilmesi. Fareler kaçmak ya da yakındaki yüzeylere tutmayın ki böyle bir konumda, teyp onların kuyrukları tarafından askıya alınır. Bu test sırasında, tipik olarak altı dakika süre içinde ortaya çıkan kaçış yönelik davranışları sayısal vardır. Tail-süspansiyon test ilaç keşfi prospektif antidepresan bileşiklerinin yüksek verimli tarama için değerli bir araçtır. Burada, biz, ortaya çıkabilir ve bunları önlemek için nasıl potansiyel sorunlar hakkında ek vurgu ile bu testin uygulanması için gerekli ayrıntılar anlatılmaktadır. Biz de yaygın olarak kullanılan C57BL / 6 gibi kuyruk tırmanma davranışına bir çözüm, ortak bir sorun, bazı fare suşları bu testi işe yaramaz hale getirir. Özellikle, küçük bir plastik ile fare kuyrukları geçerek kuyruk tırmanma davranışları önlemekönce süspansiyon silindir. Son olarak, ayrıntılarıyla nasıl bu test tecelli davranışları elle puan.

Watch this Scientific Journal Video about The Tail Suspension Test at JoVE.com

The Tail Suspension Test

Tail-süspansiyon test farelerde antidepresan ilaç tedavilerinin etkinliğini değerlendirmek için deneysel bir prosedür olarak onaylanmıştır. Fareler altı dakika için onların kuyrukları tarafından askıya alınır ve kaçış ilgili davranışlar değerlendirilmektedir. Biz kuyruk süspansiyon test yürütülmesinde kullanılan yöntem açıklanmaktadır.

Kuyruk Süspansiyon Testi

The tail-suspension test is a mouse behavioral test useful in the screening of potential antidepressant drugs, and assessing of other manipulations that ...

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