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DOI :
10.3791/3778-v
April 9th, 2012
Chapters
0:05
Title
1:28
Preparing CFA-emulsified Antigen
3:08
Immunization of Donor Mice
3:51
Isolation of Lymph Node Cells after Immunization and Re-stimulation with Antigen
6:07
Harvest of Re-stimulated Lymph Node Cells and Adoptive Transfer
6:47
Antigenic Challenge of Recipient Mice and Development of Disease
7:31
Results: Kinetics of Disease Progression Following Immunization, Adoptive Transfer and Challenge
7:58
Conclusion
特定のマウス系統では、ミエリン塩基性タンパク質で実験的自己免疫性脳脊髄炎(EAE)の誘導に抵抗することができます。ここで説明するには、無反応を逆にし、いくつかの典型的なEAE耐性マウスの汚れに麻痺性疾患を引き起こす単純な予防接種プロトコルです。
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