Name: rapid determination of the thermal nociceptive threshold in diabetic rats
Uploaded: 2012-05-17T13:00:00
Description: Watch this Scientific Journal Video about Rapid Determination of the Thermal Nociceptive Threshold in Diabetic Rats at JoVE.com

Questo lavoro è stato finanziato dalla American Diabetes Association (ADA), Grant JF1-10-14 (MDiF). Vorremmo ringraziare il personale del Laboratorio delle Risorse Animali presso WSU. Gli autori ringraziano l&#39;assistenza con l&#39;analisi statistica dei dati da Neil Paton, Ph.D.

TNTs di topi e ratti sono determinati utilizzando il incrementale piastra riscaldante analgesia metro [iHPAM, IITC Inc. Life Science (Woodland Hills, CA)]. L&#39;apparecchiatura consiste di diversi componenti: una piastra di alluminio (10 x 20 cm) con il sistema di riscaldamento sotto osservazione e una camera di plexiglass sopra, una unità di controllo termico, software di acquisizione dati (Serie # IITC parte morbida 8), un PC (personal computer) e un pedale per l&#39;avviamento a distanza, fermare o ripristinare il ...

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Analogamente al test classico piatto caldo per quantificare iperalgesia termica 4,13, il dosaggio di nocicezione qui descritto consente in modo rapido e affidabile per quantificare il comportamento nocifensive in ratti e topi. Tuttavia, contrariamente a test classico, il incrementale hot-plate metodo è non invasivo e praticamente priva di stress. Sebbene alcuni trattenere è necessario eseguire il test (cioè, l&#39;animale deve essere nella camera di osservazione), ratti o topi sono abituati ad are...

<table border="1"><tbody><tr><td> Nome </td><td> Azienda </td><td> Numero di catalogo </td></tr><tr><td> Incrementale Hot-Plate Analgesia Meter </td><td> IITC Inc. Life Science </td><td> Part # PE34 </td></tr><tr><td> Serie soft 8 </td><td> IITC Inc. Life Science </td><td> Part # Series8 </td></tr><tr><td> Streptozotocina </td><td> Calbiochem </td><td> 572201 </td></tr></tbody></table>

rapid determination of the thermal nociceptive threshold in diabetic rats

Neuropatia diabetica dolorosa (PDN) è caratterizzata da iperalgesia cioè, aumento della sensibilità agli stimoli nocivi, e cioè allodinia, ipersensibilità a stimoli normalmente innocui 1. Iperalgesia e allodinia sono stati studiati in molti modelli di roditori diversi di diabete mellito 2. Tuttavia, come affermato da Bölcskei et al, la determinazione del &quot;dolore&quot; in modelli animali è difficile a causa della sua natura soggettiva 3. Inoltre, i metodi tradizionali utilizzati per determinare le risposte comportamentali a stimoli termici nocivi di solito mancano di riproducibilità e 3 sensibilità farmacologica. Per esempio, utilizzando il metodo della piastra calda di Ankier 4, sussulto, ritiro e / o leccare di una posteriore e / o le zampe anteriori è quantificato come latenze reflex a costanti stimoli termici elevati (52-55 ° C). Tuttavia, gli animali che sono iperalgesico di stimolo termico non riproducibile mostrato differenze di relatenze Flex utilizzando le sovra-temperature soglia 3,5. Come il metodo descritto di recente Bölcskei et al. 6, le procedure descritte qui consente la determinazione rapida, sensibile e riproducibile di soglie nocicettivi termici (TNTs) in topi e ratti. Il metodo utilizza aumentando lentamente stimolo termico applicato soprattutto alla pelle di topo / ratto superficie plantare. Il metodo è particolarmente sensibile per studiare anti-nocicezione durante stati iperalgesiche come PDN. Le procedure descritte sotto sono basate su quelle pubblicate in dettaglio da Almasi et al 5 e Bölcskei et al 3. Le procedure descritte qui sono stati approvati la cura degli animali da laboratorio e Comitato uso (LACUC), Wright State University.

Watch this Scientific Journal Video about Rapid Determination of the Thermal Nociceptive Threshold in Diabetic Rats at JoVE.com

Rapid Determination of the Thermal Nociceptive Threshold in Diabetic Rats

Qui, descriviamo una procedura rapida, semplice e affidabile per determinare la temperatura minima alla quale i ratti o topi mostrare un comportamento nocifensive,<em&gt; Ie</em&gt; Il<em&gt; Soglia termica nocicettiva</em&gt; (TNT). Questo metodo si applica uno stimolo lento aumento termico permettendo stima preciso e riproducibile di TNTs con minimo eventuali sollecitazioni agli animali.

Rapida determinazione della soglia termica nocicettivo in ratti diabetici

Painful diabetic neuropathy (PDN) is characterized by hyperalgesia i.e., increased sensitivity to noxious stimulus, and allodynia i.e., hypersensitivity ...

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