Name: protocol for long duration whole body hyperthermia in mice
Uploaded: 2012-08-25T12:00:00
Description: Watch this Scientific Journal Video about Protocol for Long Duration Whole Body Hyperthermia in Mice at JoVE.com

1。动物伦理在执行长持续时间热疗对小鼠确保机构动物管理和使用委员会已批准的程序。本文讨论的所有程序，并显示在影随行的国家免疫学研究所实验动物伦理委员会的批准。 2。了解热疗全身热疗（WBH）被传递放置在预热的网箱孵化器被放置在保持在39.5℃下的动物在标准化的协议，一个温度传感器，在小鼠体内的外科手术植入，?...

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<html>早在5000 BC，，埃及医生治疗肿瘤热。希腊人认识到，在一些医疗护理的热值。最古老的文本，摩西提到的“温泉（创世记36:24），，治疗提高身体的温度。 早期的技术使用气相微生物提取物诱导发热或热水浴诱导热疗1。然而，这些方法可以很容易地监视或控制。在我们的协议小鼠暴露于高热的控制和可重复的方式。 在此过程中，重要的是对...

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protocol for long duration whole body hyperthermia in mice

热疗是一个通用术语，用来定义在核心体温高于正常的增加。它经常被用于描述增加芯体的温度的过程中观察到发热。热疗作为佐剂的使用已经出现作为一个有前途的癌症生物学领域中的肿瘤消退的过程。用于此目的，最重要的要求是具有可靠和均匀的加热协议。我们已经开发出了一个协议，用于热疗（全身）的小鼠。在这个协议中，动物被暴露于高温的周期为90分钟，然后通过15分钟的休息时间。在此期间，小鼠很容易获得食物和水。在最初的几个的热疗曝光周期的小鼠体温高尖峰，防止固定的动物。此外，施用生理盐水前几个周期中的脱水的影响减到最小。这个协议可以模拟条件下的小鼠12-24小时出现发烧一样。我们有使用8-12周龄BALB / Cj的雌性小鼠证明协议。

Watch this Scientific Journal Video about Protocol for Long Duration Whole Body Hyperthermia in Mice at JoVE.com

Protocol for Long Duration Whole Body Hyperthermia in Mice

本文介绍了一种协议，用于全身热疗的小鼠发烧一样，可以刺激条件下可达12-24小时。

持续时间较长的小鼠全身热疗协议

Hyperthermia is a general term used to define the increase in core body temperature above normal. It is often used to describe the increased core body ...

Research

JoVE Journal

Medicine

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科研

医学

Parabiosis in Mice: A Detailed Protocol

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Long-term Blood Pressure Measurement in Freely Moving Mice Using Telemetry

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Body Composition and Metabolic Caging Analysis in High Fat Fed Mice

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金属异物在儿童体内摄取的手持金属探测器筛选

Coins are the most common ingested metallic foreign bodies among children. The goal of this protocol is to assess the accuracy and feasibility of using a ...

Whole Body and Regional Quantification of Active Human Brown Adipose Tissue Using 18F-FDG PET/CT

Whole Body and Regional Quantification of Active Human Brown Adipose Tissue Using 18F-FDG PET/CT

18F-fdg Petct 对活性人棕色脂肪组织的全身和区域定量

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In Vitro and In Vivo Delivery of Magnetic Nanoparticle Hyperthermia Using a Custom-Built Delivery System

使用定制的输送系统进行磁性纳米颗粒热疗的体外和体内输送

Hyperthermia has long been used in the treatment of cancer. Techniques have varied from the intra-tumoral insertion of hot iron rods, to systemically ...