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DOI :
10.3791/3804-v
April 3rd, 2012
Chapters
0:05
Title
1:28
Infecting Cells with Retrovirus Expression Reprogramming Factors
3:31
Isolation and Expansion of Induced Pluripotent Stem Cells
4:42
Analysis of Pluripotent Markers by Immunofluorescence and Quantitative Real-time PCR
6:06
Representative Cell-reprogramming Results
8:03
Conclusion
的方法来产生人类诱导多能干细胞(iPS细胞)通过逆转录病毒介导的异位表达Oct4的,SOX2的,KLF4和MYC的描述。基于GFP的表达,以确定人类IPSC的殖民地一个切实可行的办法进行了讨论。
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