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DOI :
10.3791/3807-v
February 8th, 2012
Chapters
0:05
Title
1:39
Titration of Mutagen
5:59
Enrichment of Egress Mutants
8:48
Validation of Mutant Phenotypes by Egress Assays
12:41
Representative Data
10:21
Cloning of Egress Mutants by Limiting Dilution
14:14
Conclusion
앞으로 유전학이 어떻게 분자 수준을 해명하기위한 강력한 방법입니다 Toxoplasma는 숙주 세포에서 egresses. 프로토콜은 화학적으로 기생충을 mutagenize 위해 제공, 유도 탈출구 결함있는 돌연변이에 대한 풍부하고, 복제된 돌연변이 표현형을 검증하고 있습니다.
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