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DOI :
10.3791/3810-v
March 6th, 2012
Chapters
0:05
Title
1:32
Preparation of 3H-cholesterol
1:53
Label Cellular Cholesterol
2:37
Equilibrium Incubation
3:19
Cholesterol Efflux Incubation
4:10
Processing Samples
5:45
Data Analysis
6:11
Representative Results: Assaying Modulators of Cholesterol Efflux in Macrophages
7:32
Conclusion
胆固醇检测到定量,从培养细胞的胆固醇流出率和血浆受体的能力,接受从细胞释放的胆固醇。该法由标记细胞内池和胆固醇释放到细胞外受体之间的胆固醇,胆固醇平衡。
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