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DOI :
10.3791/3823-v
April 2nd, 2012
Chapters
0:05
Title
1:52
Isolation and Culture of SCG Neurons from Rat Embryos
5:56
Infection of SCG Cultures with HSV-1 Us11-EGFP and Establishment of Latency
8:35
Reactivation and Assessment
9:45
Results: HSV-1 Reactivation
11:19
Conclusion
协议描述了一种高效和可重复性的模型系统研究单纯疱疹病毒1型(HSV-1)延迟和重新启动。该法采用同质交感神经神经元文化,并允许使用各种工具,包括RNA干扰和重组蛋白表达的病毒神经元相互作用的分子解剖。
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