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11:01 min
January 11th, 2012
DOI :
10.3791/3830-v
Chapters
0:05
Title
1:09
Culture of hNPCs in a PuraMatrix
3:41
Immunocytochemical Staining of Entire Matrices
5:44
Release of hNPCs from the Scaffolds for Flow-cytometry Analysis
7:04
Quantification of βIII-tubulin Positive Cells by Flow Cytometry
8:09
Observation and Enumeration of PuraMatrix Encapsulated hNPCs
10:37
Conclusion
Summary
在这里,我们描述了自组装的三维支架文化人类神经祖细胞的使用。我们提出了一个协议,以流式细胞仪分析后如释放支架的细胞。该协议可能会调整到其他类型的细胞,进行详细的机械研究。
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