Name: experimental endocarditis model of methicillin resistant staphylococcus aureus (mrsa) in rat
Uploaded: 2012-06-04T12:00:00
Description: Watch this Scientific Journal Video about Experimental Endocarditis Model of Methicillin Resistant Staphylococcus aureus MRSA in Rat at JoVE.com

Dette arbejde blev støttet af det amerikanske National Institutes of Health [tilskud R01AI-39.108 til ASB] og American Heart Association [tilskud SDG 0630219N og AID 09GRNT2180065 til YQX].

1. Klargøring af MRSA-stammer for Infektion <ol><li> Inokulere en løkkefuld af MRSA kultur fra en bestand rør ved -80 ° C til en fåreblod Trypticase Soy Agar (TSA) plade (se tabel specifikke reagenser og udstyr), og der inkuberes ved 37 ° C natten over. </li><li> Kontroller renheden af ​​kulturen på blodagar plade (tilsvarende koloni fænotyper) for at sikre, at der ikke er nogen forurening. </li><li> Vælge en koloni fra fåreblod TSA plade og inokulere koloni i 5 ml Trypticase Soy Broth (TSB) i et 15 ml s...

<ol>
	<li>Petti, C. A., Fowler, V. G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Staphylococcus+aureus+bacteremia+and+endocarditis.">Staphylococcus aureus bacteremia and endocarditis.</a> Cardiol. Clin. 21, 219-233 (2003).</li><li>Bashore, T. M., Cabell, C., Fowler, V. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Update+on+infective+endocarditis.">Update on infective endocarditis.</a> Curr. Probl. Cardiol. 31, 274-352 (2006).</li><li>Fowler, V. G., Justice, A., Moore, C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Risk+factors+for+hematogenous+complications+of+intravascular+catheter-associated+Staphylococcus+aureus+bacteremia.">Risk factors for hematogenous complications of intravascular catheter-associated Staphylococcus aureus bacteremia.</a> Clin. Infect. Dis. 17, 313-320 (1993).</li><li>Wisplinghoff, H., Bischoff, T., Tallent, S. M., Seifert, H., Wenzel, R. P., Edmond, M. B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Nosocomial+bloodstream+infections+in+US+hospitals:+analysis+of+24,179+cases+from+a+prospective+nationwide+surveillance+study.">Nosocomial bloodstream infections in US hospitals: analysis of 24,179 cases from a prospective nationwide surveillance study.</a> Clin. Infect. Dis. 39, 309-317 (2004).</li><li>Hoen, B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Special+issues+in+the+management+of+infective+endocarditis+caused+by+gram-positive+cocci.">Special issues in the management of infective endocarditis caused by gram-positive cocci.</a> Infect Dis. Clin. North Am. 16, 437-452 (2002).</li><li>Moise, P. A., Hershberger, E., Amodio-Groton, M. I., Lamp, K. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Safety+and+clinical+outcomes+when+utilizing+high-dose+(&gt;+or+=8+mg/kg)+daptomycin+therapy.">Safety and clinical outcomes when utilizing high-dose (&gt; or =8 mg/kg) daptomycin therapy.</a> Ann. Pharmacother. 43, 1211-1219 (2009).</li><li>Sakoulas, G., Brown, J., Lamp, K. C., Friedrich, L. V., Lindfield, K. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Clinical+outcomes+of+patients+receiving+daptomycin+for+the+treatment+of+Staphylococcus+aureus+infections+and+assessment+of+clinical+factors+for+daptomycin+failure:+a+retrospective+cohort+study+utilizing+the+Cubicin+Outcomes+Registry+and+Experience.">Clinical outcomes of patients receiving daptomycin for the treatment of Staphylococcus aureus infections and assessment of clinical factors for daptomycin failure: a retrospective cohort study utilizing the Cubicin Outcomes Registry and Experience.</a> Clin. Ther. 31, 1936-1945 (2009).</li><li>Xiong, Y. Q., Willard, J., Kadurugamuwa, J. L., Yu, J., Francis, K. P., Bayer, A. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Real-time+in+vivo+bioluminescent+imaging+for+evaluating+the+efficacy+of+antibiotics+in+a+rat+Staphylococcus+aureus+endocarditis+model.">Real-time in vivo bioluminescent imaging for evaluating the efficacy of antibiotics in a rat Staphylococcus aureus endocarditis model.</a> Antimicrob. Agents Chemother. 49, 380-387 (2005).</li><li>Peerschke, E. I., Bayer, A. S., Ghebrehiwet, B., Xiong, Y. Q. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=gC1qR/p33+blockade+reduces+Staphylococcus+aureus+colonization+of+target+tissues+in+an+animal+model+of+infective+endocarditis.">gC1qR/p33 blockade reduces Staphylococcus aureus colonization of target tissues in an animal model of infective endocarditis.</a> Infect. Immun. 74, 4418-4423 (2006).</li><li>Ganesh, V. K., Rivera, J. J., Smeds, E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+structural+model+of+the+Staphylococcus+aureus+ClfA-fibrinogen+interaction+opens+new+avenues+for+the+design+of+anti-staphylococcal+therapeutics.">A structural model of the Staphylococcus aureus ClfA-fibrinogen interaction opens new avenues for the design of anti-staphylococcal therapeutics.</a> PLoS Pathog. 4, e1000226 (2008).</li><li>Boles, B. R., Horswill, A. R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Staphylococcal+biofilm+disassembly.">Staphylococcal biofilm disassembly.</a> Trends Microbiol. , (2011).</li><li>Tseng, C. W., Sanchez-Martinez, M., Arruda, A., Liu, G. Y. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Subcutaneous+Infection+of+Methicillin+Resistant+Staphylococcus+Aureus+(MRSA).">Subcutaneous Infection of Methicillin Resistant Staphylococcus Aureus (MRSA).</a> J. Vis. Exp. (48), e2528 (2011).</li></ol>

Rotten endocarditis er en vigtig og velkarakteriserede dyremodeller for in vivo-undersøgelser i patogenesen og antimikrobielle midler i behandlingen af bakterieinfektioner 9-11. Desuden repræsenterer rotte endocarditis model sammensat af akutte og sub-akutte infektioner, og nøje efterligner menneskelige modstykke og human indfødte ventil endocarditis. Desuden, ledsaget af en bolig kateter, det repræsenterer en klassisk biofilm-associeret infektion, en fælles og vanskeligt spørgsmål i kliniske...

<table border="1"><tbody><tr><td> Navn på reagenset </td><td> Firma </td><td> Katalog nummer </td><td> Kommentarer </td></tr><tr><td> Blodagarplade, 5% fåreblod i Trypticase Soy Agar (TSA) </td><td> Hardy Diagnostics </td><td> A10BX </td><td></td></tr><tr><td> Trypticase Soy Broth (TSB) </td><td> Becton Dickinson </td><td> 211825 </td><td></td></tr><tr><td> Rysteinkubator </td><td> LABNET </td><td> I5311-DS </td><td></td></tr><tr><td> Polyethylenrør </td><td> Becton Dickinson </td><td> 427401 </td><td></td></tr><tr><td> Kateterindføringsorganet </td><td> Becton Dickinson </td><td> 6999 </td><td></td></tr><tr><td></td><td></td><td></td><td></td></tr><tr><td> Isofluorance </td><td> Vestlige Medical Suppy, Inc </td><td> 2147 </td><td> Følg sikkerhed og håndtering af information </td></tr><tr><td> Kirurgiske instrumenter </td><td> Fin Science Tools og biomedicinsk forskning Instruments, Inc. </td><td></td><td> Find de instrumenter fra de to selskaber til dine behov </td></tr></tbody></table>

experimental endocarditis model of methicillin resistant staphylococcus aureus (mrsa) in rat

Endovaskulære infektioner, herunder endocarditis, er livstruende infektionssygdomme syndromer 1-3. Staphylococcus aureus er den mest almindelige verdensplan årsagen til sådanne syndromer med uacceptabelt høj morbiditet og mortalitet selv med passende antimikrobielle middel behandlinger 4-6. Stigningen i infektioner på grund af methicillin-resistente S. aureus (MRSA), de høje vancomycin kliniske behandling fejl og voksende problemer med linezolid og daptomycin modstand har alle yderligere kompliceret behandling af patienter med sådanne infektioner, og førte til høje udgifter til sundhedsvæsenet 7, 8. Desuden bør det understreges, at de seneste undersøgelser med antibiotika behandlingsresultater er baseret på kliniske indstillinger, og dermed kan meget vel blive påvirket af værtfaktorer varierende fra patient til patient. Derfor er en relevant dyremodel for endovaskulær infektion, hvor værtsfaktorer svarer fra dyr til dyr, er mereafgørende at undersøge mikrobiel patogenese, såvel som virkningen af ​​hidtil ukendte antimikrobielle midler. Endocarditis hos rotter er en veletableret eksperimentel dyremodel, der nøje tilnærmer human nativ ventil endocarditis. Denne model er blevet anvendt til at undersøge rollen af ​​visse stafylokok virulensfaktorer og effektiviteten af ​​antibiotika behandlingsregimer for stafylokok endocarditis. I denne rapport beskriver vi den eksperimentelle endocarditis modellen på grund af MRSA, der kunne bruges til at undersøge bakteriel patogenese og respons på antibiotisk behandling.

Watch this Scientific Journal Video about Experimental Endocarditis Model of Methicillin Resistant Staphylococcus aureus MRSA in Rat at JoVE.com

Experimental Endocarditis Model of Methicillin Resistant Staphylococcus aureus MRSA in Rat

Eksperimentel rotte endocarditis model på grund af methicillin-resistente<em&gt; S. aureus</em&gt;.

Eksperimentel Endocarditis Model af Methicillin Resistent<em&gt; Staphylococcus aureus</em&gt; (MRSA) i rotte

Endovascular infections, including endocarditis, are life-threatening infectious syndromes1-3. Staphylococcus aureus is the most common world-wide cause ...

Research

JoVE Journal

Immunology and Infection

Experimental Endocarditis Model of Methicillin Resistant Staphylococcus aureus (MRSA) in Rat

The Use of Drip Flow and Rotating Disk Reactors for Staphylococcus aureus Biofilm Analysis

The Use of Drip Flow and Rotating Disk Reactors for Staphylococcus aureus Biofilm Analysis

Most microbes in nature are thought to exist as surface-associated communities in biofilms.1  Bacterial biofilms are encased within a matrix and attached ...

Subcutaneous Infection of Methicillin Resistant Staphylococcus Aureus (MRSA)

Subcutaneous Infection of Methicillin Resistant Staphylococcus Aureus MRSA

MRSA is a worldwide threat to public health, and MRSA skin and soft-tissue infections now account for more than half of all soft-tissue infections in the ...

Overcoming Unresponsiveness in Experimental Autoimmune Encephalomyelitis (EAE) Resistant Mouse Strains by Adoptive Transfer and Antigenic Challenge

Overcoming Unresponsiveness in Experimental Autoimmune Encephalomyelitis EAE Resistant Mouse Strains by Adoptive Transfer and Antigenic Challenge

Overvinde unresponsiveness i eksperimentel autoimmun encephalomyelitis (EAE) Resistente musestammer ved adoptiv overførsel og antigenudfordring

Experimental autoimmune encephalomyelitis (EAE) is an inflammatory disease of the central nervous system (CNS) and has been used as an animal model for ...

Staphylococcus aureus Growth using Human Hemoglobin as an Iron Source

Staphylococcus aureus Growth using Human Hemoglobin as an Iron Source

Staphylococcus aureus Vækst med humant hæmoglobin som en jernkilde

S. aureus is a pathogenic bacterium that requires iron to  carry out vital metabolic functions and cause disease. The most abundant  reservoir of iron ...

Multiplex PCR Assay for Typing of Staphylococcal Cassette Chromosome Mec Types I to V in Methicillin-resistant Staphylococcus aureus

Multiplex PCR Assay for Typing of Staphylococcal Cassette Chromosome Mec Types I to V in Methicillin-resistant Staphylococcus aureus

Multiplex PCR Assay til Typing af stafylokokker Cassette Kromosom Mec Typer I til V i Methicillin-resistent<em&gt; Staphylococcus aureus</em

Staphylococcal Cassette Chromosome mec (SCCmec) typing  is a very important molecular tool for understanding the epidemiology and  clonal strain ...

Studying Interactions of Staphylococcus aureus with Neutrophils by Flow Cytometry and Time Lapse Microscopy

Studying Interactions of Staphylococcus aureus with Neutrophils by Flow Cytometry and Time Lapse Microscopy

Studere Interaktioner af<em&gt; Staphylococcus aureus</em&gt; Med Neutrophils ved flowcytometri og Time Lapse Microscopy

We present methods to study the effect of phenol soluble modulins (PSMs)  and other toxins produced and secreted by Staphylococcus  aureus on neutrophils. ...

Targeting Biofilm Associated Staphylococcus aureus Using Resazurin Based Drug-susceptibility Assay

Targeting Biofilm Associated Staphylococcus aureus Using Resazurin Based Drug-susceptibility Assay

Målretning Biofilm Associated<em&gt; Staphylococcus aureus</em&gt; Brug Resazurin Baseret Drug-modtagelighed Assay

Most pathogenic bacteria are able to form biofilms during infection, but due to the difficulty of manipulating and assessing biofilms, the vast majority ...

Genotyping of Staphylococcus aureus by Ribosomal Spacer PCR (RS-PCR)

Genotyping of Staphylococcus aureus by Ribosomal Spacer PCR RS-PCR

genotypebestemmelse af<em&gt; Staphylococcus aureus</em&gt; ved Ribosomal Spacer PCR (RS-PCR)

The ribosomal spacer PCR (RS-PCR) is a highly resolving and robust genotyping method for S. aureus that allows a high throughput at moderate costs and is, ...

Methodology for the Study of Horizontal Gene Transfer in Staphylococcus aureus

Methodology for the Study of Horizontal Gene Transfer in Staphylococcus aureus

Metode for Studiet af horisontal genoverførsel i<em&gt; Staphylococcus aureus</em

One important feature of the major opportunistic human pathogen Staphylococcus aureus is its extraordinary ability to rapidly acquire resistance to ...

A Mouse Model to Assess Innate Immune Response to Staphylococcus aureus Infection

A Mouse Model to Assess Innate Immune Response to Staphylococcus aureus Infection

En musemodel at vurdere medfødte immunrespons for Staphylococcus aureus infektioner

Staphylococcus aureus (S. aureus)&#160;infections, including methicillin resistant stains, are an enormous burden on the healthcare system. With incidence ...