Name: blood collection for biochemical analysis in adult zebrafish
Uploaded: 2012-05-26T14:00:00
Description: Watch this Scientific Journal Video about Blood Collection for Biochemical Analysis in Adult Zebrafish at JoVE.com

FIPE / HCPA - 丰杜Incentivo 1 PesquisaéEventos 短斗篷- <a href="http://www.capes.gov.br/">CoordenaçãoAperfeiçoamento的Pessoal的Nível高级</a>

1。协议文本<ol><li>之前收集斑马鱼的血液，这是必要的准备麻醉测定的水。水族箱〜200毫升倒入一个500毫升容量的容器。新增〜200克的冰碴儿。温度应约4°C。由于冰块融化，这将是必要的，添加更多的冰块，以保持近4°C的恒温</li><li>当麻醉测定的水已准备就绪，准备采血所需的材料。提出保留一个低尖上P20的移液器和离开的地方可以很容易地访问的移液器。不要让枪头接触任何污染来源。 </li...

<ol>
	<li>Schneider, A. C. R., dos Santo, J. L., Porawski, M., Schaefer, P. G., Maurer, R. L., Matte, U., da Silveira, T. R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Implementa&#231;&#227;o+de+um+novo+modelo+de+experimenta&#231;&#227;o+animal+Zebrafish.">Implementa&#231;&#227;o de um novo modelo de experimenta&#231;&#227;o animal Zebrafish.</a> Rev. HCPA. 29, 100-103 (2009).</li><li>Briggs, J. P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+zebrafish:+a+new+model+organism+for+integrative+physiology.">The zebrafish: a new model organism for integrative physiology.</a> Am. J. Physiol. Regulatory Integrative Comp. Physiol. 282, 3-9 (2002).</li><li>Menke, A. L., Sptsbergen, J. M., Wolterbeek, A. P. M., Woutersen, R. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Normal+anatomy+and+histology+of+adult+Zebrafish.">Normal anatomy and histology of adult Zebrafish.</a> Toxicologic Pathology. 000, 1-16 (2011).</li><li>Murtha, J. M., Qi, W., Keller, E. T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Hematologic+and+serum+biochemical+values+for+Zebrafish.">Hematologic and serum biochemical values for Zebrafish.</a> Comp. Med. 53, 37-41 (2003).</li><li>Jagadeeswaran, P., Sheehan, J. P., Craig, F. E., Troyer, D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Identification+and+characterization+of+zebrafish+thrombocytes.">Identification and characterization of zebrafish thrombocytes.</a> Br. J. Haematol. 107, 731-738 (1999).</li><li>Jagadeeswaran, P., Sheehan, J. P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Analysis+of+blood+coagulation+in+the+zebrafish.">Analysis of blood coagulation in the zebrafish.</a> Blood Cells Mol. Dis. 25, 239-249 (1999).</li><li>Eames, S. C., Philipson, L. H., Prince, V. E., Kinkel, M. D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Blood+sugar+measurement+in+zebrafish+reveals+dynamics+of+glucose+homeostasis.">Blood sugar measurement in zebrafish reveals dynamics of glucose homeostasis.</a> Zebrafish. 7, 205-213 (2010).</li><li>Kilpatrick, E. S., Rumley, A. G., Rumley, C. N. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+effect+of+haemolysis+on+blood+glucose+meter+measurement.">The effect of haemolysis on blood glucose meter measurement.</a> Diabet. Med. 12, 341-343 (1995).</li><li>Gupta, T., Mullins, M. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Dissection+of+Organs+from+the+Adult+Zebrafish.">Dissection of Organs from the Adult Zebrafish.</a> J. Vis. Exp. (37), e1717 (2010).</li><li>Kinkel, M. D., Eames, S. C., Philipson, L. H., Prince, V. E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Intraperitoneal+Injection+into+Adult+Zebrafish.">Intraperitoneal Injection into Adult Zebrafish.</a> J. Vis. Exp. (42), e2126 (2010).</li><li>Pugach, E. K., Li, P., White, R., Zon, L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Retro-orbital+Injection+in+Adult+Zebrafish.">Retro-orbital Injection in Adult Zebrafish.</a> J. Vis. Exp. (34), e1645 (2009).</li></ol>

本文提出了一种简单的技术，允许进一步的血液和血清分析斑马​​鱼实验。这种技术具有潜力，有助于未来的斑马鱼血液学研究血液中的参数数据。它也应该让更多的斑马鱼作为实验模型的应用。 这种技术不需要特殊技能或实施精确技术。此外，它可实现高达两倍的血液量相对其他技术收集，从而允许使用较少的鱼，以获得所需的生物材料。该技术有一个关键的一步，这?...

<table border="1"><tbody><tr><td> 试剂名称 </td><td> 公司 </td><td> 目录编号 </td><td> 评论 </td></tr><tr><td>低保留的提示</td><td>应用生物系统公司</td><td> 022493020 </td><td></td></tr><tr><td>的Eppendorf离心机5415D </td><td> Eppendorf公司</td><td>已停产</td><td></td></tr></tbody></table>

blood collection for biochemical analysis in adult zebrafish

斑马鱼已被用作研究一些人类疾病的动物模型。它可以作为一个强大的临床前平台的分子事件的研究和治疗策略，以及评估某些疾病的生理机制1。 有成年斑马鱼的生理器官和系统，这可能导致研究人员推断，让斑马鱼系统的勘探所需的基本技术缺乏相对较少的出版物。斑马鱼血液生化值均在2003年首次报道，默撒和同事4人聘用了第一由Jagadeeswaran和他的同事在1999年描述的采血技术。简单地说，血液收集通过微吸管尖端通过外侧切口，长度约0.3厘米，背主动脉的地区5。分钟尺寸的参与，这是因为是一种高精度的技术要求非常熟练的医生。在同组的另一发表在同年6使用相同的技术。在2010年，埃姆斯和他的同事评估整个血糖水平，在斑马鱼7。他们获得了血，用剪刀执行​​斩首和肝素微毛细管收集管插入到胸鳍关节1。他们提到基尔帕特里克工作等为基础的温度适当的存储解决困难与溶血8。当试图在我们的实验室，使用Jagadeeswaran的技术，我们发现这是很难做出正确的地方正是切口，不要让相当数量的血液丢失，才可以开始收集。 近日，Gupta等9描述了如何解剖成年斑马鱼器官中，Kinkle等10描述了如何执行intraperitonEAL注射，Pugach等11描述了如何执行眼窝注射。然而，更多的工作需要更充分地探讨在斑马鱼研究的基本技术。 斑马鱼体积小，使用它作为实验模型的研究人员面临的挑战。此外，由于这种规模狭小，重要的是简单的技术开发，以使研究人员能够探索的斑马鱼模型的优势。

Watch this Scientific Journal Video about Blood Collection for Biochemical Analysis in Adult Zebrafish at JoVE.com

Blood Collection for Biochemical Analysis in Adult Zebrafish

本文提出了一种从斑马鱼背主动脉采血技术。它还提供了用于生化分析，如确定胆固醇和甘油三酯水平的试验，取得血清的指示。

在成年斑马鱼的生化分析采血车

The zebrafish has been used as an animal model for studies of several human diseases. It can serve as a powerful preclinical platform for studies of ...

Research

JoVE Journal

Biology

Zebrafish Brain Ventricle Injection

斑马鱼脑室注射液

Proper brain ventricle formation during embryonic brain development is required for normal brain function.  Brain ventricles are the highly conserved ...

科研

生物学

Retro-orbital Injection in Adult Zebrafish

在成年斑马鱼的眼窝射出

Drug treatment of whole animals is an essential tool in any model system for pharmacological and chemical genetic studies. Intravenous (IV) injection is ...

In vivo Electroporation of Morpholinos into the Adult Zebrafish Retina

In vivo Electroporation of Morpholinos into the Adult Zebrafish Retina

在体内电穿孔Morpholinos到成年斑马鱼视网膜

Many devastating inherited eye diseases result in progressive and irreversible blindness because humans cannot regenerate dying or diseased retinal ...

In vivo Electroporation of Morpholinos into the Regenerating Adult Zebrafish Tail Fin

In vivo Electroporation of Morpholinos into the Regenerating Adult Zebrafish Tail Fin

再生的成年斑马鱼尾鳍在吗啉代入体内电穿孔

Certain species of urodeles and teleost fish can regenerate their tissues. Zebrafish have become a widely used model to study the spontaneous regeneration ...

Gavaging Adult Zebrafish

Gavaging成年斑马鱼

The zebrafish has become an important in vivo model in biomedical research. Effective methods must be developed and utilized to deliver compounds or ...

An Assay for Lateral Line Regeneration in Adult Zebrafish

对于侧线再生成年斑马鱼的含量

Due to the clinical importance of hearing and balance disorders in man, model organisms such as the zebrafish have been used to study lateral line ...

Analysis of Nephron Composition and Function in the Adult Zebrafish Kidney

肾单位的组成与功能的成年斑马鱼肾脏分析

The zebrafish model has emerged as a relevant system to study kidney development, regeneration and disease. Both the embryonic and adult zebrafish kidneys ...

Repeated Blood Collection for Blood Tests in Adult Zebrafish

重复采血车在成年斑马鱼验血

Repeated blood collection is one of the most common techniques performed on laboratory animals. However, a non-lethal protocol for blood collection from ...

Immunofluorescence Analysis of Endogenous and Exogenous Centromere-kinetochore Proteins

内源性的免疫荧光分析及外源着丝粒着丝粒蛋白

"Centromeres" and "kinetochores" refer to the site where chromosomes associate with the spindle during cell division. Direct visualization of ...

In Vivo Surface Electrocardiography for Adult Zebrafish

在 Vivo 表面心电图中成人斑马鱼

The electrocardiogram waveforms of adult zebrafish and those of humans are remarkably similar. These electrocardiogram similarities enhance the value of ...