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24.3K Views
•
08:30 min
May 16th, 2012
DOI :
10.3791/3874-v
Chapters
0:05
Title
1:51
Prostate Sample Collection
2:40
Isolating Total RNA from Samples
4:27
Real-time PCR for Detection of miRNA
6:10
Analyzing the Data
7:25
Representative qPCR Results
8:02
Conclusion
Summary
实时定量聚合酶链反应(QPCR)是一种快速,灵敏的方法来探讨不同的microRNA(miRNA)的分子的表达水平在肿瘤样本。使用此方法数百种不同的miRNA分子表达可以被放大,量化,并从相同的cDNA模板分析。
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