Sunnybrook Health Sciences Centre, Toronto, Canada

2 ARTICLES PUBLISHED IN JoVE

Medicine

MicroRNA Detection in Prostate Tumors by Quantitative Real-time PCR (qPCR)

Aida Gordanpour¹, Robert K. Nam², Linda Sugar³, Stephanie Bacopulos¹, Arun Seth^1,3,4

¹Department of Laboratory Medicine & Pathobiology, University of Toronto, ²Division of Urology, Sunnybrook Health Sciences Centre, Toronto, Canada, ³Department of Anatomic Pathology, Sunnybrook Health Sciences Centre, Toronto, Canada, ⁴Biological Sciences, Sunnybrook Research Institute

Quantitative Real Time polymerase chain reaction (qPCR) is a rapid and sensitive method to investigate the expression levels of various microRNA (miRNA) molecules in tumor samples. Using this method expression of hundreds of different miRNA molecules can be amplified, quantified, and analyzed from the same cDNA template.

Biology

Reconstruction of 3-Dimensional Histology Volume and its Application to Study Mouse Mammary Glands

Rushin Shojaii¹, Stephanie Bacopulos^2,3, Wenyi Yang^2,3, Tigran Karavardanyan⁴, Demetri Spyropoulos⁵, Afshin Raouf⁶, Anne Martel^1,4, Arun Seth^2,3

¹Department of Medical Biophysics, University of Toronto, ²Platform Biological Sciences, Sunnybrook Research Institute, ³Department of Laboratory Medicine and Pathobiology, University of Toronto, ⁴Physical Sciences, Sunnybrook Research Institute, ⁵Department of Pathology and Laboratory Medicine, Medical University of South Carolina, ⁶Manitoba Institute of Cell Biology, University of Manitoba

We present an image registration approach for 3-dimensional (3D) histology volume reconstruction, which facilitates the study of the changes of an organ at the level of macrostructures made up of cells . Using this approach, we studied the 3D changes between wild-type and Igfbp7-null mammary glands.