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DOI :
10.3791/3891-v
February 20th, 2013
Chapters
0:05
Title
1:43
PALeO-TimeCourse: Protease-catalyzed Labeling of Proteolytic Cleavages
2:43
PALeO-TimeCourse: Postdigestion Labeling of Proteolytic Termini
3:48
ALeO-TimeCourse: Acid-catalyzed Labeling of Carboxyl Groups
6:35
Preparation of Spectral Time and 18O-incorporation Plots
7:06
Representative Results
8:58
Conclusion
4:25
MALDI-TOF/TOF MS/MS Data Acquisition and Analysis
稳定同位素标记的工作流程,采用 18 O-富氧水(LEO-工作流)的多用途工具进行定量和定性的蛋白质组学研究。在蛋白酶辅助(古)的工作流程, 18 O原子的引入,通过蛋白水解和羧基氧交换反应介导的蛋白酶。在酸催化(ALEO)工作流程中, 18 O原子在低pH条件下引入羧基氧交换。
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