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DOI :
10.3791/3892-v
•
16:10 min
March 22nd, 2012
Chapters
0:05
Title
2:59
Transfection Using Amaxa Mouse T Cell Nucleofector Kit
6:07
TIRF Setup
10:24
Imaging
13:19
Representative Results
14:54
Conclusion
条块分割,无论是在细胞膜或蛋白质进入细胞内的位置是一个监管机制,可以极大地影响信号的成果;因此,了解信号,重要的是要学习的时间和空间的行为,所涉及的蛋白质。我们描述1 TIRF显微镜为基础的系统研究在T细胞信号转导在这里的,而且是广泛适用的。
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