Name: saliva, salivary gland, and hemolymph collection from ixodes scapularis ticks
Uploaded: 2012-02-21T13:00:00
Description: Watch this Scientific Journal Video about Saliva, Salivary Gland, and Hemolymph Collection from Ixodes scapularis Ticks at JoVE.com

作者要感谢病媒传播疾病的动物资源科司，特别是安德烈彼得森，丽莎马苏迪，贝尔纳奥布莱恩，约翰·利德尔的老鼠和兔子的关心和维护。我们也想感谢艾米·乌尔曼，特里萨·拉塞尔，和Barbara J.约翰逊朝着这个手稿的贡献。最后，我们想承认艾丽萨埃克特通信在疾病预防控制中心副主任办公室指导拍摄这个手稿与所有相关的合法性产生的插图和朱迪·拉维尔。

1。幻灯片准备的淋巴集合 （电影1） <ol><li>动物和外用3％过氧化氢的地方，轻轻地从积极喂养蜱5分钟，然后在70％乙醇10分钟，到表面消毒。 </li><li>与PAP笔硅烷涂层显微镜幻灯片上绘制一个圆，并放置在PAP笔圈的刻度。 <ol><li>硅烷涂层幻灯片用最好的淋巴坚持显微镜幻灯片。 </li></ol></li><li>在解剖显微镜下（1X目标，10X目镜，放大倍率3.5X）蜱。 </li><li>用钳子轻轻地往下推?...

<ol>
	<li>Quach, K. A., Boctor, F. N., Elston, D. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=What's+eating+you?+Hyalomma+ticks.">What's eating you? Hyalomma ticks.</a> Cutis. 87, 165-167 (2011).</li><li>Graham, J., Stockley, K., Goldman, R. D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Tick-borne+illnesses:+a+CME+update.">Tick-borne illnesses: a CME update.</a> Pediatr. Emerg. Care. 27, 141-147 (2011).</li><li>Nuttall, P. A., Paesen, G. C., Lawrie, C. H., Wang, H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Vector-host+interactions+in+disease+transmission.">Vector-host interactions in disease transmission.</a> J. Mol. Microbiol. Biotechnol. 2, 381-386 (2000).</li><li>Estrada-Pena, A., Jongejan, F. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Ticks+feeding+on+humans:+a+review+of+records+on+human-biting+Ixodoidea+with+special+reference+to+pathogen+transmission.">Ticks feeding on humans: a review of records on human-biting Ixodoidea with special reference to pathogen transmission.</a> Exp. Appl. Acarol. 23, 685-715 (1999).</li><li>Nuttall, P. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Pathogen-tick-host+interactions:+Borrelia+burgdorferi+and+TBE+virus.">Pathogen-tick-host interactions: Borrelia burgdorferi and TBE virus.</a> Zentralbl Bakteriol. 289, 492-505 (1999).</li><li>Jones, L. D., Hodgson, E., Nuttall, P. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Enhancement+of+virus+transmission+by+tick+salivary+glands.">Enhancement of virus transmission by tick salivary glands.</a> J. Gen. Virol. 70, 1895-1898 (1989).</li><li>Labuda, M., Nuttall, P. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Tick-borne+viruses.">Tick-borne viruses.</a> Parasitol. 129, 221-245 (2004).</li><li>Socolovschi, C., Mediannikov, O., Raoult, D., Parola, P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Update+on+tick-borne+bacterial+diseases+in+Europe.">Update on tick-borne bacterial diseases in Europe.</a> Parasite. 16, 259-273 (2009).</li><li>Zhang, L., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Molecular+Interactions+that+Enable+Movement+of+the+Lyme+Disease+Agent+from+the+Tick+Gut+into+the+Hemolymph.">Molecular Interactions that Enable Movement of the Lyme Disease Agent from the Tick Gut into the Hemolymph.</a> PLoS Pathog. 7, e1002079 (2011).</li><li>Piesman, J., Schneider, B. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Dynamic+changes+in+Lyme+disease+spirochetes+during+transmission+by+nymphal+ticks.">Dynamic changes in Lyme disease spirochetes during transmission by nymphal ticks.</a> Exp. Appl. Acarol. 28, 141-145 (2002).</li><li>Brossard, M., Wikel, S. K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Tick+immunobiology.">Tick immunobiology.</a> Parasitol. , S161-S176 (2004).</li><li>Machackova, M., Obornik, M., Kopecky, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Effect+of+salivary+gland+extract+from+Ixodes+ricinus+ticks+on+the+proliferation+of+Borrelia+burgdorferi+sensu+stricto+in+vivo.">Effect of salivary gland extract from Ixodes ricinus ticks on the proliferation of Borrelia burgdorferi sensu stricto in vivo.</a> Folia Parasitol. 53, 153-158 (2006).</li><li>Nuttall, P. A., Labuda, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Tick-host+interactions:+saliva-activated+transmission.">Tick-host interactions: saliva-activated transmission.</a> Parasitol. 129, 177-189 (2004).</li><li>Anguita, J., Hedrick, M. N., Fikrig, E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Adaptation+of+Borrelia+burgdorferi+in+the+tick+and+the+mammalian+host.">Adaptation of Borrelia burgdorferi in the tick and the mammalian host.</a> FEMS Microbiol. Rev. 27, 493-504 (2003).</li><li>Hovius, J. W., van Dam, A. P., Fikrig, E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Tick-host-pathogen+interactions+in+Lyme+borreliosis.">Tick-host-pathogen interactions in Lyme borreliosis.</a> Trends Parasitol. 23, 434-438 (2007).</li><li>Dunham-Ems, S. M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Live+imaging+reveals+a+biphasic+mode+of+dissemination+of+Borrelia+burgdorferi+within+ticks.">Live imaging reveals a biphasic mode of dissemination of Borrelia burgdorferi within ticks.</a> Journal Clin. Invest. 119, 3652-3665 (2009).</li><li>Ribeiro, J. M., Mather, T. N., Piesman, J., Spielman, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Dissemination+and+salivary+delivery+of+Lyme+disease+spirochetes+in+vector+ticks+(Acari:+Ixodidae).">Dissemination and salivary delivery of Lyme disease spirochetes in vector ticks (Acari: Ixodidae).</a> J. Med. Entomol. 24, 201-205 (1987).</li><li>Piesman, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Transmission+of+Lyme+disease+spirochetes+(Borrelia+burgdorferi.">Transmission of Lyme disease spirochetes (Borrelia burgdorferi.</a> Exp. Appl. Acarol. 7, 71-80 (1989).</li><li>De Silva, A. M., Fikrig, E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Growth+and+migration+of+Borrelia+burgdorferi+in+Ixodes+ticks+during+blood+feeding.">Growth and migration of Borrelia burgdorferi in Ixodes ticks during blood feeding.</a> Am. J. Trop. Med. Hyg. 53, 397-404 (1995).</li><li>Horka, H., Cerna-Kyckova, K., Skallova, A., Kopecky, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Tick+saliva+affects+both+proliferation+and+distribution+of+Borrelia+burgdorferi+spirochetes+in+mouse+organs+and+increases+transmission+of+spirochetes+to+ticks.">Tick saliva affects both proliferation and distribution of Borrelia burgdorferi spirochetes in mouse organs and increases transmission of spirochetes to ticks.</a> Int. J. Med. Microbiol. 299, 373-380 (2009).</li><li>Brossard, M., Wikel, S. K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Immunology+of+interactions+between+ticks+and+hosts.">Immunology of interactions between ticks and hosts.</a> Med. Vet. Entomol. 11, 270-276 (1997).</li><li>Wikel, S. K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Tick+modulation+of+host+immunity:+an+important+factor+in+pathogen+transmission.">Tick modulation of host immunity: an important factor in pathogen transmission.</a> Int. J. Parasitol. 29 (99), 851-859 (1999).</li><li>Binnington, K. C., Kemp, D. H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Role+of+tick+salivary+glands+in+feeding+and+disease+transmission.">Role of tick salivary glands in feeding and disease transmission.</a> Adv. Parasitol. 18, 315-339 (1980).</li><li>Guo, X., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Inhibition+of+neutrophil+function+by+two+tick+salivary+proteins.">Inhibition of neutrophil function by two tick salivary proteins.</a> Infect. Immun. 77, 2320-2329 (2009).</li><li>Montgomery, R. R., Lusitani, D., De Boisfleury Chevance, A., Malawista, S. E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Tick+saliva+reduces+adherence+and+area+of+human+neutrophils.">Tick saliva reduces adherence and area of human neutrophils.</a> Infect. Immun. 72, 2989-2994 (2004).</li><li>Lima, C. M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Differential+infectivity+of+the+Lyme+disease+spirochete+Borrelia+burgdorferi+derived+from+Ixodes+scapularis+salivary+glands+and+midgut.">Differential infectivity of the Lyme disease spirochete Borrelia burgdorferi derived from Ixodes scapularis salivary glands and midgut.</a> J. Med. Entomol. 42, 506-510 (2005).</li><li>Severinova, J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Co-inoculation+of+Borrelia+afzelii+with+tick+salivary+gland+extract+influences+distribution+of+immunocompetent+cells+in+the+skin+and+lymph+nodes+of+mice.">Co-inoculation of Borrelia afzelii with tick salivary gland extract influences distribution of immunocompetent cells in the skin and lymph nodes of mice.</a> Folia Microbiol. 50, 457-463 (2005).</li><li>Labuda, M., Jones, L. D., Williams, T., Nuttall, P. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Enhancement+of+tick-borne+encephalitis+virus+transmission+by+tick+salivary+gland+extracts.">Enhancement of tick-borne encephalitis virus transmission by tick salivary gland extracts.</a> Med. Vet. Entomol. 7, 193-196 (1993).</li><li>Kariu, T., Coleman, A. S., Anderson, J. F., Pal, U. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Methods+for+Rapid+Transfer+and+Localization+of+Lyme+Disease+Pathogens+Within+the+Tick+Gut.">Methods for Rapid Transfer and Localization of Lyme Disease Pathogens Within the Tick Gut.</a> J. Vis. Exp. (48), e2544 (2011).</li><li>Edwards, K. T., Goddard, J., Varela-Stokes, A. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Examination+of+the+internal+morphology+of+the+Ixodid+tick+Amblyomma+maculatum+koch,+(Acari:Ixodidae);+a+"How-to"+pictorial+dissection+guide.">Examination of the internal morphology of the Ixodid tick Amblyomma maculatum koch, (Acari:Ixodidae); a "How-to" pictorial dissection guide.</a> Midsouth Entomologist. 2, 28-39 (2009).</li><li>Ledin, K. E., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Borreliacidal+activity+of+saliva+of+the+tick+Amblyomma+americanum.">Borreliacidal activity of saliva of the tick Amblyomma americanum.</a> Med. Vet. Entomol. 19, 90-95 (2005).</li><li>Ribeiro, J. M., Zeidner, N. S., Ledin, K., Dolan, M. C., Mather, T. N. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=How+much+pilocarpine+contaminates+pilocarpine-induced+tick+saliva?.">How much pilocarpine contaminates pilocarpine-induced tick saliva?.</a> Med. Vet. Entomol. 18, 20-24 (2004).</li><li>Barker, R. W., Burris, E., Sauer, J. R., Hair, J. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Composition+of+tick+oral+secretions+obtained+by+three+different+collection+methods.">Composition of tick oral secretions obtained by three different collection methods.</a> J. Med. Entomol. 10, 198-201 (1973).</li><li>Burgdorfer, W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Hemolymph+test.+A+technique+for+detection+of+rickettsiae+in+ticks.">Hemolymph test. A technique for detection of rickettsiae in ticks.</a> Am. J. Trop. Med. Hyg. 19, 1010-1014 (1970).</li></ol>

蜱血淋巴，唾液腺和唾液的收集是非常重要的研究蜱传病原体传播，流行，传播，扩散，并在双方的滴答和主机6,11-13,20,23,29的持久性。有几种方法剖析蜱30,31的 。然而，当收集唾液腺关键是正确剖析蜱唾液腺不破裂，或在蜱的遗体丢失。一旦被删除要洗几次，清除肠污染，然后可以固定获得唾液腺提取物（SGE）染色或PBS地面幻灯片上，他们需要从蜱唾液腺。上海黄金交易所是更?...

<table border="1"><tbody><tr><td> 试剂 </td><td> 公司 </td><td> 目录编号 </td></tr><tr><td>过氧化氢</td><td>费舍尔</td><td> H312-500 </td></tr><tr><td>乙醇</td><td>阿库罗斯福冈</td><td> 61509-5000 </td></tr><tr><td> PBS </td><td>波士顿生物制品</td><td> BM-2205 </td></tr><tr><td>杜蒙精细镊子（3C） </td><td>费舍尔</td><td> NC9906085 </td></tr><tr><td>硅烷偶联剂处理的载玻片</td><td> bioworld </td><td> 42763007-1 </td></tr><tr><td>子宫颈抹片笔</td><td> bioworld </td><td> 21750008-1 </td></tr><tr><td>超级霜加显微镜载玻片</td><td>费舍尔</td><td> 12-550-18 </td></tr><tr><td>匹罗卡品</td><td>西格玛</td><td> P6503-5G </td></tr><tr><td>蛋白酶抑制剂</td><td>西格玛</td><td> P2714 </td></tr><tr><td> ＃11一次性手术刀</td><td>羽毛</td><td> 2975＃ </td></tr><tr><td>无毒橡皮泥</td><td&gt;费舍尔</td><td> S17307 </td></tr><tr><td>毛细管</td><td>大通科研玻璃，公司</td><td> 40A502 </td></tr></tbody></table>

saliva, salivary gland, and hemolymph collection from ixodes scapularis ticks

蜱遍布世界各地，并与许多蜱传疾病的折磨人类。蜱是莱姆病和蜱传回归热（ 伯氏菌），洛矶山斑疹热（ 立克次氏体 ），埃立克体病（ 查菲埃立克体和E.球菌 ），无形体病（ 嗜吞噬细胞无形体 ），脑炎（蜱的病原体引起的载体森林脑炎病毒），巴贝斯虫病（ 巴贝斯虫属），科罗拉多蜱热（Colti病毒），兔热病（ 弗朗西斯tularensis）1-8。要正确传输到主机，这些传染性病原体的差异调节基因表达，与蜱蛋白质交互，并通过蜱3,9-13迁移。例如，莱姆病代理， 莱姆病 ，蜱的地方性周期14,15盛宴和饥荒阶段适应，通过基因差异表达。此外，作为硬蜱蜱消耗吸血伯从肠到的hemocoel，他们前往唾液腺，并传送到主机与被驱逐的唾液9,16-19复制和迁移。 由于蜱送入主机通常响应与一个强大的止血和先天免疫反应11,13,20-22。尽管这些宿主反应， 一肩胛可以养活了好几天，因为蜱唾液中含有的免疫调节的蛋白质，溶解剂，抗凝血剂，并fibrinolysins帮助蜱喂养3,11,20,21,23。由蜱唾液或唾液腺提取物（SGE）具有免疫调节活动方便，众多的蜱传病原体3,20,24-27的传输，扩散和传播。为了进一步了解如何蜱传播的传染性病原体引起的疾病，它是必不可少的积极剖析饲养蜱和收集蜱唾液。这个视频协议演示的夹层技术从积极喂养一血淋巴的收集和唾液腺的去除肩胛若虫后，48和72小时后鼠标的位置。我们还表明，从一成年女性唾液收集肩胛蜱。

Watch this Scientific Journal Video about Saliva, Salivary Gland, and Hemolymph Collection from Ixodes scapularis Ticks at JoVE.com

Saliva, Salivary Gland, and Hemolymph Collection from Ixodes scapularis Ticks

蜱血淋巴感染，唾液腺和唾液的收集是非常重要的研究蜱传病原体引起疾病。在这个协议中，我们演示了如何收集喂血淋巴和唾液腺<em&gt;肩突硬蜱</em&gt;若虫。我们还表明，从女性的唾液收集<em&gt;一。肩胛</em&gt;成年人。

唾液，唾液腺，淋巴收集肩突硬蜱蜱

Ticks are found worldwide and afflict humans with many tick-borne illnesses. Ticks are vectors for pathogens that cause Lyme disease and tick-borne ...

Research

JoVE Journal

Immunology and Infection

Saliva, Salivary Gland, and Hemolymph Collection from Ixodes scapularis Ticks

RNA Interference in Ticks

RNA干扰在蜱

Ticks are obligate hematophagous ectoparasites of wild and domestic animals and humans, and are considered to be second worldwide to mosquitoes as vectors ...

科研

免疫与感染

Hybridization in situ of Salivary Glands, Ovaries, and Embryos of Vector Mosquitoes

Hybridization in situ of Salivary Glands, Ovaries, and Embryos of Vector Mosquitoes

杂交<em&gt;原位</em&gt;和蚊虫唾液腺，卵巢，胚胎

Mosquitoes are vectors for a diverse set of pathogens including arboviruses, protozoan parasites and nematodes.  Investigation of transcripts and gene ...

Feeding of Ticks on Animals for Transmission and Xenodiagnosis in Lyme Disease Research

蜱对动物饲养的传输和Xenodiagnosis在莱姆病研究

Transmission of the etiologic agent of Lyme disease, Borrelia burgdorferi, occurs by the  attachment and blood feeding of Ixodes species ticks on ...

Developing a Salivary Antibody Multiplex Immunoassay to Measure Human Exposure to Environmental Pathogens

制定唾液抗体多重免疫测定来测量人体暴露于环境中的病原体

The etiology and impacts of human exposure to environmental pathogens are of major concern worldwide and, thus, the ability to assess exposure and ...

Rearing Ixodes scapularis, the Black-legged Tick: Feeding Immature Stages on Mice

Rearing Ixodes scapularis, the Black-legged Tick: Feeding Immature Stages on Mice

饲养<em&gt;肩突硬蜱，</em&gt;黑腿蜱：饲养对小鼠未成熟阶段

Ixodes scapularis, the vector of Lyme disease, is one of the most important disease vectors in the eastern and Midwestern United States. This species is a ...

A Method for the Measurement of Salivary Gland Function in Mice

小鼠涎腺功能的测定方法

Patients with Sj&#246;gren&#39;s syndrome, an autoimmune disease affecting the exocrine glands, develop salivary gland inflammation and have reduced ...

Preparation of Murine Submandibular Salivary Gland for Upright Intravital Microscopy

直立活体显微术制备小鼠颌下腺涎腺

The submandibular salivary gland (SMG) is one of the three major salivary glands, and is of interest for many different fields of biological research, ...

Isolation of Salivary Epithelial Cells from Human Salivary Glands for In Vitro Growth as Salispheres or Monolayers

从人类唾液腺中分离唾液表皮细胞,作为脂质层或单层体进行体外生长

The salivary glands are a site of significant interest for researchers interested in multiple aspects of human disease. One goal of researchers is to ...

Purification of High Yield Extracellular Vesicle Preparations Away from Virus

高产细胞外囊泡制剂的纯化远离病毒

One of the major hurdles in the field of extracellular vesicle (EV) research today is the ability to achieve purified EV preparations in a viral infection ...

Disclosing Hemolymph Collection and Inoculation of Metarhizium Blastospores into Rhipicephalus Microplus Ticks Towards Invertebrate Pathology Studies

Disclosing Hemolymph Collection and Inoculation of Metarhizium Blastospores into Rhipicephalus Microplus Ticks Towards Invertebrate Pathology Studies

在无脊椎动物病理学研究中发现血淋巴收集和接种转移到大黄微加分中

Ticks are obligate hematophagous ectoparasites and Rhipicephalus microplus has great importance in veterinary medicine because it causes anemia, weight ...