Name: saliva, salivary gland, and hemolymph collection from ixodes scapularis ticks
Uploaded: 2012-02-21T13:00:00
Description: Watch this Scientific Journal Video about Saliva, Salivary Gland, and Hemolymph Collection from Ixodes scapularis Ticks at JoVE.com

De auteurs willen graag de afdeling Vector-Borne Diseases Animal Resources Branch bedanken, in het bijzonder Andrea Peterson, Lisa Massoudi, Verna O&#39;Brien, en John Liddell voor hun verzorging en het onderhoud van de muizen en konijnen. We willen ook graag naar Amy Ullmann, Theresa Russell, en Barbara J. Johnson bedanken voor hun bijdragen in de richting van dit manuscript. Tot slot willen we graag Alissa Eckert erkennen in het Bureau van de Associate Director voor Communicatie aan de CDC voor de productie van de grafische illustraties en Judy Lavelle voor het richten van alle wettigheid in verband met het filmen van dit manuscript.

1. Hemolymfe collectie voor het prepareren van objectglaasjes (Film 1) <ol><li> Verwijder actief voeden teken van een dier en plaats deze in 3% actuele waterstofperoxide gedurende 5 minuten en dan in 70% ethanol gedurende 10 minuten naar de oppervlakte te steriliseren. </li><li> Met een pap pen teken een cirkel op een silaan gecoate microscoopglaasje en plaats de teek binnen de pap pen cirkel. <ol><li> Silane gecoate glaasjes worden gebruikt voor de beste hechting van de hemolymfe de micro...

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De collectie van teken hemolymfe, speekselklieren, en speeksel is van belang in de studie van door teken overgedragen pathogeen transmissie, prevalentie, verspreiding, proliferatie en persistentie in zowel de teek en de host 6,11-13,20,23,29. Er zijn verschillende manieren om een teek 30,31 ontleden. Echter, bij het verzamelen van de speekselklieren is het van cruciaal belang voor de teek ontleden goed, zodat de speekselklieren zijn niet gescheurd of verloren in overblijfselen van de teek. Als de s...

<table border="1"><tbody><tr><td> Reagens </td><td> Vennootschap </td><td> Catalogus nummer </td></tr><tr><td> Waterstofperoxide </td><td> Visser </td><td> H312-500 </td></tr><tr><td> Ethanol </td><td> Acros </td><td> 61509-5000 </td></tr><tr><td> PBS </td><td> Boston Bioproducts </td><td> BM-2205 </td></tr><tr><td> Dumont Fine pincet (3C) </td><td> Visser </td><td> NC9906085 </td></tr><tr><td> Silaan behandelde objectglaasjes </td><td> Bioworld </td><td> 42763007-1 </td></tr><tr><td> Pap pen </td><td> Bioworld </td><td> 21750008-1 </td></tr><tr><td> Super plus vorst objectglaasjes </td><td> Visser </td><td> 12-550-18 </td></tr><tr><td> Pilocarpine </td><td> Sigma </td><td> P6503-5G </td></tr><tr><td> Proteaseremmer cocktail </td><td> Sigma </td><td> P2714 </td></tr><tr><td> # 11 wegwerp scalpel </td><td> Veer </td><td> 2975 # 11 </td></tr><tr><td> Niet giftig boetseerklei </td><td&gt; Fisher </td><td> S17307 </td></tr><tr><td> Capillaire buizen </td><td> Chase wetenschappelijke Glas, inc </td><td> 40A502 </td></tr></tbody></table>

saliva, salivary gland, and hemolymph collection from ixodes scapularis ticks

Teken komen wereldwijd voor en treffen mensen met veel tick-borne ziekten. Teken zijn vectoren voor ziekteverwekkers die de ziekte van Lyme en tick-borne relapsing fever (Borrelia spp.), Rocky Mountain spotted fever (Rickettsia rickettsii), ehrlichiosis (Ehrlichia chaffeensis en E. equi), anaplasmose (Anaplasma phagocytophilum), encefalitis (tick-veroorzaken encefalitis-virus), babesiose (Babesia spp.), Colorado tick fever (Coltivirus) en tularemie (Francisella tularensis) 1-8. Om goed te worden overgebracht in de gastheer van deze infectieuze agentia differentieel reguleren van genexpressie, interactie met tik eiwitten en migreren door de teek 3,9-13. Bijvoorbeeld, de ziekte van Lyme agent, Borrelia burgdorferi, past zich door middel van differentiële genexpressie tot het feest en hongersnood stadia van de teek enzoötische cyclus van 14,15. Bovendien, als een Ixodes teek verbruikt eenbloedmeel Borrelia vermenigvuldigen en migreren van de middendarm in de hemocoel, waar ze reizen naar de speekselklieren en worden verzonden naar de host met de verdreven speeksel 9,16-19. Als een teek voedt de gastheer reageert doorgaans met een sterke hemostatische en aangeboren immuunrespons 11,13,20-22. Ondanks deze gastheer responsen, I. scapularis kan voeden voor meerdere dagen, want teek speeksel bevat eiwitten die zijn immunomodulerende, lytische middelen, anticoagulantia, en fibrinolysins om de teek voeden 3,11,20,21,23 te helpen. De immunomodulerende activiteiten bezeten door teek speeksel of speekselklier extract (SGE) te vergemakkelijken transmissie, proliferatie, en de verspreiding van tal van tick-borne pathogenen 3,20,24-27. Om verder te begrijpen hoe door teken overgedragen ziekteverwekkers ziekte te veroorzaken is het belangrijk om actief te ontleden voeden teken en door teken speeksel op te vangen. Dit protocol video toont ontleding techniekenhet verzamelen van hemolymfe en de verwijdering van speekselklieren van actief voeden I. scapularis nimfen na 48 en 72 uur na de muis plaatsing. We tonen ook aan speeksel collectie van een volwassen vrouwtje I. scapularis teek.

Watch this Scientific Journal Video about Saliva, Salivary Gland, and Hemolymph Collection from Ixodes scapularis Ticks at JoVE.com

Saliva, Salivary Gland, and Hemolymph Collection from Ixodes scapularis Ticks

De collectie van besmette teek hemolymfe, speekselklieren, en speeksel is van belang om te onderzoeken hoe door teken overgedragen pathogenen ziekte te veroorzaken. In dit protocol laten we zien hoe hemolymfe en speekselklieren te verzamelen uit de voeding<em&gt; Ixodes scapularis</em&gt; Nimfen. We tonen ook aan speeksel collectie van vrouwelijke<em&gt; I. scapularis</em&gt; Volwassenen.

Speeksel, speekselklieren en hemolymfe Collectie van Ixodes scapularis Teken

Ticks are found worldwide and afflict humans with many tick-borne illnesses. Ticks are vectors for pathogens that cause Lyme disease and tick-borne ...

Research

JoVE Journal

Immunology and Infection

Saliva, Salivary Gland, and Hemolymph Collection from Ixodes scapularis Ticks

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